Information on EC 2.3.1.256 - N-terminal methionine Nalpha-acetyltransferase NatC

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
2.3.1.256
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RECOMMENDED NAME
GeneOntology No.
N-terminal methionine Nalpha-acetyltransferase NatC
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + an N-terminal-L-methionyl-L-isoleucyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[protein] + CoA
show the reaction diagram
(2)
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acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA
show the reaction diagram
(1)
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-
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acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA
show the reaction diagram
(3)
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acetyl-CoA + an N-terminal-L-methionyl-L-tryptophyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-tryptophyl-[protein] + CoA
show the reaction diagram
(4)
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acetyl-CoA + an N-terminal-L-methionyl-L-tyrosinyl-[protein] = an N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[protein] + CoA
show the reaction diagram
(5)
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Ac/N-end rule pathway
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:N-terminal-Met-Leu/Ile/Phe/Trp/Tyr-[protein] Met Nalpha-acetyltransferase
N-terminal-acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus and makes the N-terminal residue larger and more hydrophobic, and may also play a role in membrane targeting and gene silencing. The NatC complex is found in all eukaryotic organisms, and specifically targets N-terminal L-methionine residues attached to bulky hydrophobic residues at the second position, including Leu, Ile, Phe, Trp, and Tyr residues.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an N-terminal-L-methionyl-L-histidyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-histidyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-isoleucyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[Arl8b protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Arl8b protein] + CoA
show the reaction diagram
high activity, the full sequence is MLALISRRWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[L-A double-stranded RNA virus major coat protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[L-A double-stranded RNA virus major coat protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[mTOR protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[mTOR protein] + CoA
show the reaction diagram
highest activity. The full sequence is MLGTGPARWGRPVGRRRRPVRVYP
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-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[RNP F protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[RNP F protein] + CoA
show the reaction diagram
the full sequence is MLGTEGGRWGRPVGRRRRPVRVYP
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-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[RNP H protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[RNP H protein] + CoA
show the reaction diagram
high activity, the full sequence is MLGTEGGRWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-methionyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-methionyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-tryptophyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-tryptophyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-tyrosinyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + L-Met-Ile-Arg-Leu-Lys-Ala
?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-isoleucyl-[Lrg1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[Lrg1 protein] + CoA
show the reaction diagram
acetyl-CoA + N-terminal-L-methionyl-L-isoleucyl-[Sly41 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[Sly41 protein] + CoA
show the reaction diagram
acetyl-CoA + N-terminal-L-methionyl-L-isoleucyl-[Ymr31 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[Ymr31 protein] + CoA
show the reaction diagram
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[Bem1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Bem11 protein] + CoA
show the reaction diagram
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[Glr1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Glr1 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[Pda1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Pda1 protein] + CoA
show the reaction diagram
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-
-
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?
acetyl-CoA + N-terminal-L-methionyl-L-leucyl-[Trm1-II protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Trm1-II protein] + CoA
show the reaction diagram
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-
-
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?
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Arl3 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Arl3 protein] + CoA
show the reaction diagram
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Rfc2 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Rfc2 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Rrn11 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Rrn11 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Sec18 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Sec18 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Tma20 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Tma20 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-tyrosinyl-[Nup157 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[Nup157 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-tyrosinyl-[Pxl1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[Pxl1 protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-tyrosinyl-[Tgl1 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[Tgl1 protein] + CoA
show the reaction diagram
-
-
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?
additional information
?
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almost no activity with nuclear factor kappaB p65 (MDELFPLRWGRPVGRRRRPVRVYP), kinesin KIF4A (MKEEVKGRWGRPVGRRRRPVRVYP), and high-mobility group protein A1 (SESSSKSRWGRPVGRRRRPVRVYP)
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an N-terminal-L-methionyl-L-histidyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-histidyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-isoleucyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-isoleucyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[Arl8b protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[Arl8b protein] + CoA
show the reaction diagram
Q147X3
high activity, the full sequence is MLALISRRWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[L-A double-stranded RNA virus major coat protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[L-A double-stranded RNA virus major coat protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[mTOR protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[mTOR protein] + CoA
show the reaction diagram
Q147X3
highest activity. The full sequence is MLGTGPARWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[RNP F protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[RNP F protein] + CoA
show the reaction diagram
Q147X3
the full sequence is MLGTEGGRWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-leucyl-[RNP H protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-leucyl-[RNP H protein] + CoA
show the reaction diagram
Q147X3
high activity, the full sequence is MLGTEGGRWGRPVGRRRRPVRVYP
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-lysyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-lysyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-methionyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-methionyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + an N-terminal-L-methionyl-L-phenylalanyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-tryptophyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-tryptophyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-tyrosinyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-tyrosinyl-[protein] + CoA
show the reaction diagram
acetyl-CoA + an N-terminal-L-methionyl-L-valyl-[protein]
an N-terminal-Nalpha-acetyl-L-methionyl-L-valyl-[protein] + CoA
show the reaction diagram
-
-
-
-
?
acetyl-CoA + N-terminal-L-methionyl-L-phenylalanyl-[Arl3 protein]
N-terminal-Nalpha-acetyl-L-methionyl-L-phenylalanyl-[Arl3 protein] + CoA
show the reaction diagram
-
-
-
-
?
additional information
?
-
Q147X3
almost no activity with nuclear factor kappaB p65 (MDELFPLRWGRPVGRRRRPVRVYP), kinesin KIF4A (MKEEVKGRWGRPVGRRRRPVRVYP), and high-mobility group protein A1 (SESSSKSRWGRPVGRRRRPVRVYP)
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterotrimer
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1 * 20000 + 1 * 84000 + 1 * 10000, subunit Mak3p, Mak10p and Mak31p, calculated from amino acid sequence
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in HEK-293 cells
expressed in Saccharomyces cerevisiae strain NatCDELTA
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE