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Information on EC 2.3.1.254 - N-terminal methionine Nalpha-acetyltransferase NatB and Organism(s) Mus musculus and UniProt Accession P61600

for references in articles please use BRENDA:EC2.3.1.254
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IUBMB Comments
N-terminal acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus and makes the N-terminal residue larger and more hydrophobic, and may also play a role in membrane targeting and gene silencing. The NatB complex is found in all eukaryotic organisms, and specifically targets N-terminal L-methionine residues attached to Asn, Asp, Gln, or Glu residues at the second position.
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Mus musculus
UNIPROT: P61600 not found.
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The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
hide(3 overall reactions are displayed. Show all (4)>>)
acetyl-CoA
+
an N-terminal L-methionyl-L-asparaginyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-asparaginyl-[protein]
+
CoA
+
an N-terminal L-methionyl-L-asparaginyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-asparaginyl-[protein]
+
acetyl-CoA
+
an N-terminal L-methionyl-L-glutaminyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein]
+
CoA
+
an N-terminal L-methionyl-L-glutaminyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein]
+
acetyl-CoA
+
an N-terminal L-methionyl-L-aspartyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein]
+
CoA
+
an N-terminal L-methionyl-L-aspartyl-[protein]
=
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein]
+
Synonyms
naa20, mdm20, nat3p, cra-1, naa25, mdm20p, naa50p, n-acetyltransferase 3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N-terminal acetyltransferase B complex catalytic subunit NAA20
P61600; Q8BWZ3
UniProt
NAA20
NAA25
natB
P61600; Q8BWZ3
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:N-terminal Met-Asn/Gln/Asp/Glu-[protein] Met-Nalpha-acetyltransferase
N-terminal acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free alpha-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus and makes the N-terminal residue larger and more hydrophobic, and may also play a role in membrane targeting and gene silencing. The NatB complex is found in all eukaryotic organisms, and specifically targets N-terminal L-methionine residues attached to Asn, Asp, Gln, or Glu residues at the second position.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + N-terminal L-methionyl-L-aspartyl-[Bax]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[Bax] + CoA
show the reaction diagram
P61600; Q8BWZ3
heterologous expression of human Bax in mouse embryonic fibroblasts (MEFs). Recombinant human Bax is N-terminally (Nt-)acetylated by Naa20 and that Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of MEF cells. Human Bax displays the N-terminal Met-Asp amino-acid sequence
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + an N-terminal L-methionyl-L-asparaginyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-asparginyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-aspartyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-aspartyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutaminyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutaminyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
acetyl-CoA + an N-terminal L-methionyl-L-glutamyl-[protein]
an N-terminal Nalpha-acetyl-L-methionyl-L-glutamyl-[protein] + CoA
show the reaction diagram
P61600; Q8BWZ3
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
acetyl-CoA
P61600; Q8BWZ3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
subunits Naa20 and Naa25 of enzyme complex NatB
P61600; Q8BWZ3
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
P61600; Q8BWZ3
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
P61600; Q8BWZ3
Naa25 inactivation is embryonic lethal in homozygocity. Recombinant Bax accumulates in the mitochondria of Naa25-/- MEF cells, but does not promote cytochrome c release, suggesting that an additional step is required for full activation of Bax
physiological function
P61600; Q8BWZ3
recombinant human Bax expressed in MEF cells is N-terminal (Nt-)acetylated by Naa20p. Nt-acetylation of Bax is essential to maintain Bax in an inactive conformation in the cytosol of the cells. N-terminal acetylation modulates Bax targeting to mitochondria. Contribution of Bax Nt-acetylation to its regulation
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
NAA20_MOUSE
178
0
20368
Swiss-Prot
other Location (Reliability: 2)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
P61600; Q8BWZ3
the NatB enzymatic complex is constituted of two subunits, the NAA20 catalytic subunit and the accessory subunit NAA25
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Alves, S.; Neiri, L.; Rodrigues Chaves, S.; Trindade, V.D.; Manon, S.; Dominguez, V.; Pintado, B.; Jonckheere, V.; Van Damme, P.; Silva, R.D.; Aldabe, R.; Corte-Real, M.
N-terminal acetylation modulates Bax targeting to mitochondria
Int. J. Biochem. Cell Biol.
95
35-42
2018
Mus musculus (P61600 AND Q8BWZ3), Mus musculus, Mus musculus C57BL/6 (P61600 AND Q8BWZ3), Saccharomyces cerevisiae (Q06504 AND Q12387), Saccharomyces cerevisiae, Saccharomyces cerevisiae ATCC 204508 (Q06504 AND Q12387)
Manually annotated by BRENDA team