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Information on EC 2.3.1.250 - [Wnt protein] O-palmitoleoyl transferase and Organism(s) Drosophila melanogaster and UniProt Accession Q9VWV9

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IUBMB Comments
The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo . cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.
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This record set is specific for:
Drosophila melanogaster
UNIPROT: Q9VWV9
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The taxonomic range for the selected organisms is: Drosophila melanogaster
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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(9Z)-hexadec-9-enoyl-CoA
+
[Wnt]-L-serine
=
CoA
+
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
+
[Wnt]-L-serine
=
+
[Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
Synonyms
porcupine, porcn, mom-1, mporc, mporcd, xporc, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PORCN
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(9Z)-hexadec-9-enoyl-CoA:[Wnt]-L-serine O-hexadecenoyltransferase
The enzyme, found in animals, modifies a specific serine residue in Wnt proteins, e.g. Ser209 in human Wnt3a and Ser224 in chicken WNT1 [2,3]. The enzyme can accept C13 to C16 fatty acids in vitro, but only (9Z)-hexadecenoate modification is observed in vivo [1]. cf. EC 3.1.1.98, [Wnt protein]-O-palmitoleoyl-L-serine hydrolase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
show the reaction diagram
the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg
-
-
?
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Porc or CG6205
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
the enzyme has multiple transmembrane domains, Drosophila Porc is localized at the endoplasmic reticulum when expressed in Drosophila cells as an epitope-tagged protein
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
Drosophila Porc has an extra hydrophilic N-terminal sequence, which is not found in other Porc enzymes
physiological function
the enzyme is necessary for processing Wingless (Wg), a Drosophila Wnt (Wnt) family member. The enzyme modifies the processing of substrate Wg expressed in mammalian cells
evolution
-
the enzyme belongs to the family of membrane-bound O-acyltransferases (MBOAT), which transfer acyl groups, such as a palmitoyl group, to substrates
malfunction
-
treatment with a chemical inhibitor of acyltransferases produces defective intracellular trafficking of Wingless
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PORCN_DROME
525
9
59195
Swiss-Prot
other Location (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Porc, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in HEK-293 cells with or without co-expression of Wg
gene porc, expression of the enzyme tagged with three HA epitopes at amino acid 28. Capped porc, porc3HA28, DELTA34, DELTA66, and DELTA110 RNA are prepared by in vitro transcription and RNA is injected into Drosophila embryos
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Takada, R.; Satomi, Y.; Kurata, T.; Ueno, N.; Norioka, S.; Kondoh, H.; Takao, T.; Takada, S.
Monounsaturated fatty acid modification of Wnt p+rotein: its role in Wnt secretion
Dev. Cell
11
791-801
2006
Drosophila melanogaster, Mus musculus
Manually annotated by BRENDA team
Tanaka, K.; Okabayashi, K.; Asashima, M.; Perrimon, N.; Kadowaki, T.
The evolutionarily conserved porcupine family is involved in the processing of the Wnt family
Eur. J. Biochem.
267
4300-4311
2000
Drosophila melanogaster, Drosophila melanogaster (Q9VWV9), Caenorhabditis elegans (Q22329), Homo sapiens (Q9H237), Xenopus laevis (Q9I935), Mus musculus (Q9JJJ7), Mus musculus
Manually annotated by BRENDA team