Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 2.3.1.249 - spermidine dicoumaroyl transferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q8GYW8

for references in articles please use BRENDA:EC2.3.1.249
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
The enzyme from the plant Arabidopsis thaliana has no activity with sinapoyl-CoA (cf. EC 2.3.1.248, spermidine disinapoyl transferase).
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q8GYW8
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Arabidopsis thaliana
Synonyms
spermidine dicoumaroyl transferase, spermidine coumaroyl coa acyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BAHD acyl transferase
-
spermidine coumaroyl CoA acyltransferase
-
SYSTEMATIC NAME
IUBMB Comments
4-coumaroyl-CoA:spermidine N-(hydroxycinnamoyl)transferase
The enzyme from the plant Arabidopsis thaliana has no activity with sinapoyl-CoA (cf. EC 2.3.1.248, spermidine disinapoyl transferase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 4-coumaroyl-CoA + spermidine
2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2 4-coumaroyl-CoA + spermidine
2 CoA + N1,N8-bis(4-coumaroyl)-spermidine
show the reaction diagram
the enzyme shows spermidine coumaroyl CoA acyltransferase activity both in vitro and in vivo
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0106
4-coumaroyl-CoA
pH 7.3, 30°C, recombinant His6-tagged enzyme
0.0527
spermidine
pH 7.3, 30°C, recombinant His6-tagged enzyme
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.4
4-coumaroyl-CoA
pH 7.3, 30°C, recombinant His6-tagged enzyme
3.1
spermidine
pH 7.3, 30°C, recombinant His6-tagged enzyme
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene At2g25150
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the enzyme is expressed specifically in roots
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the enzyme is a member of the BAHD acyltransferase family
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SCT_ARATH
461
0
51751
Swiss-Prot
other Location (Reliability: 2)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His6-tagged enzyme from Escherichia coli strain BL21 Rosetta (DE3) pLysS by nickel affinity chromatography and desalting gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene At2g25150, quantitative real-time RT-PCR expression analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 Rosetta (DE3) pLysS
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Luo, J.; Fuell, C.; Parr, A.; Hill, L.; Bailey, P.; Elliott, K.; Fairhurst, S.A.; Martin, C.; Michael, A.J.
A novel polyamine acyltransferase responsible for the accumulation of spermidine conjugates in Arabidopsis seed
Plant Cell
21
318-333
2009
Arabidopsis thaliana (Q8GYW8), Arabidopsis thaliana, Arabidopsis thaliana Col-0 (Q8GYW8)
Manually annotated by BRENDA team