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Information on EC 2.3.1.234 - N6-L-threonylcarbamoyladenine synthase and Organism(s) Bacillus subtilis and UniProt Accession O05518

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IUBMB Comments
The enzyme is involved in the synthesis of N6-threonylcarbamoyladenosine37 in tRNAs, which is found in tRNAs with the anticodon NNU, i.e. tRNAIle, tRNAThr, tRNAAsn, tRNALys, tRNASer and tRNAArg .
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Bacillus subtilis
UNIPROT: O05518
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The taxonomic range for the selected organisms is: Bacillus subtilis
The enzyme appears in selected viruses and cellular organisms
Synonyms
EC 2.6.99.4, Kae1, Qri7, t(6)A synthase, t(6)A37 threonylcarbamoyladenosine biosynthesis protein KAE1, t6A synthase, tRNA N6-adenosine threonylcarbamoyltransferase, TsaD, YdiE, ygjD, more
SYNONYM
ORGANISM
UNIPROT
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LITERATURE
PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
L-threonylcarbamoyladenylate:adenine37 in tRNA N6-L-threonylcarbamoyltransferase
The enzyme is involved in the synthesis of N6-threonylcarbamoyladenosine37 in tRNAs, which is found in tRNAs with the anticodon NNU, i.e. tRNAIle, tRNAThr, tRNAAsn, tRNALys, tRNASer and tRNAArg [3].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-threonylcarbamoyladenylate + adenine37 in tRNA
AMP + N6-L-threonylcarbamoyladenine37 in tRNA
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
N6-threonylcarbamoyladenonsine biosynthesis in Bacillus subtilis requires the four proteins Ywl/TsaC, YdiB/TsaE, YdiC/TsaB and YdiE/TsaD. YwlC catalyzes the conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP and diphosphate as products. Purified L-threonylcarbamoyl-AMP is efficiently processed to N6-threonylcarbamoyladenonsine by the YdiBCE proteins in the presence of tRNA substrates. This reaction is ATP independent in vitro. Data suggest channeling of the intermediate
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lauhon, C.T.
Mechanism of N6-threonylcarbamoyladenonsine (t6A) biosynthesis: isolation and characterization of the intermediate threonylcarbamoyl-AMP
Biochemistry
51
8950-8963
2012
Bacillus subtilis (O05518), Bacillus subtilis 168 (O05518)
Manually annotated by BRENDA team