The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. The modification is important for translational reading-frame maintenance. In some species that produce hydroxywybutosine the enzyme uses 7-[2-hydroxy-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe as substrate. The enzyme also has the activity of EC 2.1.1.290, tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase .
The enzyme appears in viruses and cellular organisms
The enzyme is found only in eukaryotes, where it is involved in the biosynthesis of wybutosine, a hypermodified tricyclic base found at position 37 of certain tRNAs. The modification is important for translational reading-frame maintenance. In some species that produce hydroxywybutosine the enzyme uses 7-[2-hydroxy-3-amino-3-(methoxycarbonyl)propyl]wyosine37 in tRNAPhe as substrate. The enzyme also has the activity of EC 2.1.1.290, tRNAPhe [7-(3-amino-3-carboxypropyl)wyosine37-O]-methyltransferase [2].
Substrates: the enzyme attaches both the methyl and methoxycarbonyl groups to the aminocarboxyl side chain, to complete wybutosine formation Products: -
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S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
Substrates: the enzyme attaches both the methyl and methoxycarbonyl groups to the aminocarboxyl side chain, to complete wybutosine formation Products: -
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S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
Substrates: the enzyme catalyzes methylation of the carboxyl group of the methionine moiety in wybutosine-72 to convert it to wybutosine-58 Products: -
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S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-carboxypropyl]wyosine72 in tRNAPhe
S-adenosyl-L-homocysteine + wybutosine-72 in tRNAPhe
Substrates: the enzyme catalyzes methylation of the carboxyl group of the methionine moiety in wybutosine-72 to convert it to wybutosine-58 Products: -
Substrates: the enzyme attaches both the methyl and methoxycarbonyl groups to the aminocarboxyl side chain, to complete wybutosine formation Products: -
?
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
Substrates: the enzyme attaches both the methyl and methoxycarbonyl groups to the aminocarboxyl side chain, to complete wybutosine formation Products: -
?
S-adenosyl-L-methionine + 7-[(3S)-3-amino-3-(methoxycarbonyl)propyl]wyosine72 in tRNAPhe + CO2
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting drop vapor diffusion method, using 200 mM ammonium citrate (pH 7.0), 10 mM HEPES (pH 7.5), 20% (w/v) PEG3350, 20 mM sodium citrate, and 1% (v/v)2-propanol
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