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Information on EC 2.3.1.228 - isovaleryl-homoserine lactone synthase and Organism(s) Bradyrhizobium diazoefficiens and UniProt Accession Q89VI2

for references in articles please use BRENDA:EC2.3.1.228
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IUBMB Comments
The enzyme, found in the bacterium Bradyrhizobium japonicum, does not accept isovaleryl-[acyl-carrier protein] as acyl donor (cf. EC 2.3.1.184, acyl-homoserine-lactone synthase).
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This record set is specific for:
Bradyrhizobium diazoefficiens
UNIPROT: Q89VI2
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The taxonomic range for the selected organisms is: Bradyrhizobium diazoefficiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
iv-hsl synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IV-HSL synthase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
isovaleryl-CoA:S-adenosyl-L-methionine isovaleryltranserase (lactone-forming, methylthioadenosine-releasing)
The enzyme, found in the bacterium Bradyrhizobium japonicum, does not accept isovaleryl-[acyl-carrier protein] as acyl donor (cf. EC 2.3.1.184, acyl-homoserine-lactone synthase).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
isopentyl-CoA + S-adenosyl-L-methionine
CoA + S-methyl-5'-thioadenosine + N-isopentyl-L-homoserine lactone
show the reaction diagram
-
-
-
?
isovaleryl-ACP + S-adenosyl-L-methionine
ACP + S-methyl-5'-thioadenosine + N-isovaleryl-L-homoserine lactone
show the reaction diagram
-
-
-
?
isovaleryl-CoA + S-adenosyl-L-methionine
CoA + S-methyl-5'-thioadenosine + N-isovaleryl-L-homoserine lactone
show the reaction diagram
isoform BjaI prefers isovaleryl-CoA as a substrate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
isopentyl-CoA
competitive
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0079
isopentyl-CoA
pH 7.3, temperature not specified in the publication
0.317
isovaleryl-ACP
pH 7.3, temperature not specified in the publication
0.007
isovaleryl-CoA
pH 7.3, temperature not specified in the publication
0.039
S-adenosyl-L-methionine
pH 7.3, temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.02
isovaleryl-ACP
pH 7.3, temperature not specified in the publication
0.021
isovaleryl-CoA
pH 7.3, temperature not specified in the publication
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0063
isovaleryl-ACP
pH 7.3, temperature not specified in the publication
3
isovaleryl-CoA
pH 7.3, temperature not specified in the publication
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.021
isopentyl-CoA
Bradyrhizobium diazoefficiens
pH 7.3, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Christensen, Q.H.; Brecht, R.M.; Dudekula, D.; Greenberg, E.P.; Nagarajan, R.
Evolution of acyl-substrate recognition by a family of acyl-homoserine lactone synthases
PLoS ONE
9
e112464
2014
Bradyrhizobium diazoefficiens (Q89VI2), Bradyrhizobium diazoefficiens JCM 1083 (Q89VI2)
Manually annotated by BRENDA team