the enzyme belongs to the BAHD family of acyltrasferases, and phylogenetic analysis shows, that it is more related to the acetyl-CoA:benzylalcohol acetyltransferases than to the anthocyanin acyltransferases. Ss5MaT2 contains the BAHD conserved motifs 1 HXXXD and 3 DFGWG, but lacks motif 2 YFGNC
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native Ss5MaT2 510fold by ammonium sulfate fractionation, a first anion exchange chromatographic step, hydrophobic interaction and ceramic hydroxyapatite chromatography, gel filtration, and a second, different anion exchange chromatograpic step to over 95% purity. Recombinant N-terminally His-tagged SsAT412 from Escherichia coli strain JM109 to homogeneity
Identification and characterization of a novel anthocyanin malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme that is phylogenetically separated from other anthocyanin acyltransferases