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Information on EC 2.3.1.20 - diacylglycerol O-acyltransferase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WKC9
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2.3.1.20 diacylglycerol O-acyltransferaseIUBMB Comments
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
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Word Map
- 2.3.1.20
- triacylglycerols
- triglyceride
- adipose
- milk
- acyl-coas
- lipoprotein
- obesity
- cholesterol
- droplet
- cattle
- adipocytes
- lipase
- steatosis
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lipogenesis
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lipogenic
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high-fat
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holstein
-
oleic
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dairy
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proliferator-activated
- phosphatidate
- glycerolipids
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lipolysis
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oleaginous
- glycerol-3-phosphate
- chylomicron
-
biodiesel
-
srebf1
-
oilseed
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lipotoxicity
- stearoyl-coa
- acaca
- camelina
- industry
-
insig1
- biofuel production
-
oleosins
-
cholinephosphotransferase
- elovl6
- drug development
-
triacylglyceride
-
holstein-friesian
- sn-1,2-diacylglycerols
- analysis
- glycerolphosphate
- oleoyl-coa
- protein-1c
-
lipin
- lpaat
-
ricinoleic
- medicine
- baylyi
- chylomicronemia
- biotechnology
-
steryl
-
kennedy
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
dgat1, dgat2, dgat, diacylglycerol acyltransferase, diacylglycerol acyltransferase 1, diacylglycerol o-acyltransferase, dgat-1, monoacylglycerol acyltransferase, acyl-coa:diacylglycerol acyltransferase, diacylglycerol acyltransferase 2, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1,2-diacylglycerol acyltransferase
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acyl-CoA:1,2-dioleoyl-sn-glycerol acyltransferase
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acyltransferase, diacylglycerol
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DGAT
DGAT1
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DGAT1A
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-
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DGAT2
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DGAT2A
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diacylglycerol acyltransferase
diglyceride acyltransferase
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diglyceride O-acyltransferase
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palmitoyl-CoA-sn-1,2-diacylglycerol acyltransferase
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DGAT
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diacylglycerol acyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Acyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:1,2-diacyl-sn-glycerol O-acyltransferase
Palmitoyl-CoA and other long-chain acyl-CoAs can act as donors.
CAS REGISTRY NUMBER
COMMENTARY
9029-98-5
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
oleoyl-CoA + diolein
CoA + triolein
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-
-
?
1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
rac-tripalmitoylglycerol + CoA
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-
-
?
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + 1,2,3-triacylglycerol
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?
oleoyl-CoA + diolein
CoA + triolein
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?
additional information
?
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enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate
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-
?
additional information
?
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enzyme mediates the transesterification of diacylglycerol using long-chain acyl-CoA as acyl donors. In addition, it functions as mycolyltransferase, a docking model suggests that palmitoleoyl-coenzyme A and 1,2-dipalmitin occupy the same active site as trehalose 6,6-dimycolate and trehalose 6-monomycolate
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
acyl-CoA + 1,2-diacyl-sn-glycerol
CoA + 1,2,3-triacylglycerol
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-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
-
tgs transcript levels are upregulated under various stress culture conditions
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
-
values of enzyme activity for several isoforms
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
additional information
high DGAT activity when expressed in recombinant Escherichia coli
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
value of pH-optimum for several enzyme isoforms
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
a Rv3371 deletion mutant of Mtb shows impaired non-replicating persistence in vitro and altered sensitivity to anti-mycobacterial drugs. In low iron medium, the Rv3371 deletion mutant shows reduced formation of TAG containing extracellular vesicles
physiological function
physiological function
upon overexpression in Mycobacterium smegmatis mc2155, cell morphology is changed and the cells become grossly enlarged. A massive formation of lipid bodies and a change in lipid pattern is observed simultaneously
physiological function
the diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations during persistence. It is involved in the biosynthesis of triacylglycerol (TAG). TAG is important to mycobacteria both as cell envelope component and energy reservoir
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
-
disruption of gene tgs1, i.e. Rv3130c, leads to drastically reduced triacylglycerol accumulation
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene Rv3371, specific to pathogenic mycobacteria, leads to modifications in colony morphotype, bacterial architecture, cell surface properties, and to elevated triacylglycerol (TAG) levels when expressed in Mycolicibacterium smegmatis (Mycobacterium smegmatis)
gene tgs1, expressionin Escherichia coli, the recombinant enzyme prefers C26 : 0-CoA for triglycerol accumulation
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the Rv3371 promoter is minimally active during exponential growth in vitro, but is upregulated under stationary phase, hypoxia, nutrient starvation, nitrosative stress, low iron, in IFN-gamma activated macrophages and infected mice. The low iron-induced expression of Rv3371 is likely due to the de-repression by Rv1404, which is probably activated by ideR
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
drug development
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the enzyme is a possible target for antilatency drugs
medicine
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use of enzyme as target for antilacency drugs that prevent cells from surviving dormancy
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Daniel, J.; Deb, C.; Dubey, V.S.; Sirakova, T.D.; Abomoelak, B.; Morbidoni, H.R.; Kolattukudy, P.E.
Induction of a novel class of diacylglycerol acyltransferases and triacylglycerol accumulation in Mycobacterium tuberculosis as it goes into a dormancy-like state in culture
J. Bacteriol.
186
5017-5030
2004
Mycobacterium tuberculosis
Waeltermann, M.; Stoeveken, T.; Steinbuechel, A.
Key enzymes for biosynthesis of neutral lipid storage compounds in prokaryotes: Properties, function and occurrence of wax ester synthases/acyl-CoA:diacylglycerol acyltransferases
Biochimie
89
230-242
2007
Mycolicibacterium smegmatis, Mycobacterium tuberculosis (P9WKC3), Mycobacterium tuberculosis (P9WKC5), Mycobacterium tuberculosis (P9WKC7), Mycobacterium tuberculosis (P9WKC9), Streptomyces avermitilis (Q826D7), Acinetobacter baylyi (Q8GGG1), Streptomyces coelicolor (Q9RIU8), Mycobacterium tuberculosis H37Rv (P9WKC3), Mycobacterium tuberculosis H37Rv (P9WKC5), Mycobacterium tuberculosis H37Rv (P9WKC7), Mycobacterium tuberculosis H37Rv (P9WKC9)
Sirakova, T.D.; Dubey, V.S.; Deb, C.; Daniel, J.; Korotkova, T.A.; Abomoelak, B.; Kolattukudy, P.E.
Identification of a diacylglycerol acyltransferase gene involved in accumulation of triacylglycerol in Mycobacterium tuberculosis under stress
Microbiology
152
2717-2725
2006
Mycobacterium tuberculosis
Elamin, A.A.; Stehr, M.; Spallek, R.; Rohde, M.; Singh, M.
The Mycobacterium tuberculosis Ag85A is a novel diacylglycerol acyltransferase involved in lipid body formation
Mol. Microbiol.
81
1577-1592
2011
Mycobacterium tuberculosis (P9WQP3), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WQP3)
Rastogi, S.; Singh, A.K.; Chandra, G.; Kushwaha, P.; Pant, G.; Singh, K.; Mitra, K.; Sashidhara, K.V.; Krishnan, M.Y.
The diacylglycerol acyltransferase Rv3371 of Mycobacterium tuberculosis is required for growth arrest and involved in stress-induced cell wall alterations
Tuberculosis
104
8-19
2017
Mycobacterium tuberculosis (P9WKA9), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WKA9), Mycobacterium tuberculosis ATCC 25618 (P9WKA9)
EXTERNAL LINKS (specific for EC-Number 2.3.1.20)
Transporter Classification Database (TCDB): 2.A.50.4.1, 2.A.50.4.10
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