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Information on EC 2.3.1.198 - glycerol-3-phosphate 2-O-acyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LMM0

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IUBMB Comments
A membrane-associated enzyme required for suberin or cutin synthesis in plants. Active with a wide range of acyl-CoA substrates (C16:0-C24:0). The enzyme from some sources has much higher activity with omega-oxidized acyl-CoAs. Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9LMM0
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
sn-2 acyltransferase, glycerol-3-phosphate acyltransferase 6, ppgpat2, ppgpat4, glycerol-3-phosphate 2-o-acyltransferases, sn-2 gpat, nbgpat6a, slgpat6, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GPAT4
sn-glycerol-3-phosphate acyltransferase
-
At2g38110
glycerol-3-phosphate 2-O-acyltransferases
-
GPAT5
no additional phosphatase activity
GPAT6
sn-glycerol-3-phosphate acyltransferase
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:sn-glycerol-3-phosphate 2-O-acyltransferase
A membrane-associated enzyme required for suberin or cutin synthesis in plants. Active with a wide range of acyl-CoA substrates (C16:0-C24:0). The enzyme from some sources has much higher activity with omega-oxidized acyl-CoAs. Some enzymes are bifunctional and have an additional phosphatase activity producing sn-2-monoacylglycerols.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-omega-hydroxy-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + (9Z)-17-carboxyheptadec-9-enoyl-CoA
CoA + 1-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate + 2-((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate
show the reaction diagram
(9Z)-17-carboxyheptadec-9-enoyl-CoA = C18:1 DCA-CoA
((9Z)-17-carboxyheptadec-9-enoyl)-sn-glycerol 3-phosphate = C18:1 DCA-LPA, ratio of sn-2:sn-1-labeled products is 5:1 or greater
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-omega-hydroxy-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
sn-glycerol 3-phosphate + 15-carboxypentadecanoyl-CoA
CoA + 1-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate + 2-(15-carboxypentadecanoyl)-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-omega-hydroxy-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-acyl-sn-glycerol 3-phosphate
show the reaction diagram
dicarboxylic acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-dicarboxylic acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
omega-hydroxy-acyl-CoA + sn-glycerol 3-phosphate
CoA + 2-omega-hydroxy-acyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
synthesis of unsaturated fatty acid and double bond index (DBI) of phosphatidylglycerol of plants are inhibited by salt stress
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
activity assay at room temperature
20
activity assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
modeling data indicate this localisation
Manually annotated by BRENDA team
modeling data indicate this localisation
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
malfunction
metabolism
isozymes GPAT4 and GPAT8 are glycerol-3-phosphate 2-O acyltransferases that catalyze the first step in leaf and stem cutin acylglycerol assembly
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GPAT4_ARATH
503
3
56262
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned in yeast microsomes and wheat germ cells
gene GPAT6, sequence comparisons and phylogenetic tree, quantitative real-time PCR enzyme expression analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, W.; Pollard, M.; Li-Beisson, Y.; Beisson, F.; Feig, M.; Ohlrogge, J.
A distinct type of glycerol-3-phosphate acyltransferase with sn-2 preference and phosphatase activity producing 2-monoacylglycerol
Proc. Natl. Acad. Sci. USA
107
12040-12045
2010
Arabidopsis thaliana (O80437), Arabidopsis thaliana (Q9CAY3), Arabidopsis thaliana (Q9LMM0)
Manually annotated by BRENDA team
Sui, N.; Tian, S.; Wang, W.; Wang, M.; Fan, H.
Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis
Front. Plant Sci.
8
1337
2017
Arabidopsis thaliana (O80437), Arabidopsis thaliana Col-0 (O80437)
Manually annotated by BRENDA team
Jayawardhane, K.N.; Singer, S.D.; Weselake, R.J.; Chen, G.
Plant sn-glycerol-3-phosphate acyltransferases biocatalysts involved in the biosynthesis of intracellular and extracellular lipids
Lipids
53
469-480
2018
Arabidopsis thaliana (O80437), Arabidopsis thaliana (Q9LMM0)
Manually annotated by BRENDA team
Yang, W.; Pollard, M.; Li-Beisson, Y.; Ohlrogge, J.
Quantitative analysis of glycerol in dicarboxylic acid-rich cutins provides insights into Arabidopsis cutin structure
Phytochemistry
130
159-169
2016
Arabidopsis thaliana, Arabidopsis thaliana (Q5XF03), Arabidopsis thaliana Col-0, Arabidopsis thaliana Col-0 (Q5XF03)
Manually annotated by BRENDA team