Information on EC 2.3.1.197 - dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase

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The expected taxonomic range for this enzyme is: Aneurinibacillus thermoaerophilus

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EC NUMBER
COMMENTARY hide
2.3.1.197
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RECOMMENDED NAME
GeneOntology No.
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose = CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
dTDP-3-acetamido-alpha-D-fucose biosynthesis
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Polyketide sugar unit biosynthesis
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Biosynthesis of antibiotics
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose 3-N-acetyltransferase
The product, dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose, is a component of the glycan chain of the crystalline bacterial cell surface layer protein (S-layer glycoprotein) of Aneurinibacillus thermoaerophilus.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
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LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
CoA + dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactopyranose
show the reaction diagram
the enzyme is involved in the biosynthesis of dTDP-3-acetamido-3,6-dideoxy-alpha-D-galactose. The reverse reaction cannot be detected
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.061
acetyl-CoA
pH 7.4, 37°C
0.0667
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
pH 7.4, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.1
acetyl-CoA
pH 7.4, 37°C
2.3
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
pH 7.4, 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50.8
acetyl-CoA
pH 7.4, 37°C
3.4
dTDP-3-amino-3,6-dideoxy-alpha-D-galactopyranose
pH 7.4, 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
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LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
to increase the stability, the enzyme is concentrated by ultrafiltration before storage at 4°C or -20°C and supplemented with 50% glycerol
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4°C, the concentrated enzyme preparations are stable at 4°C for several months with only a marginal decrease of enzyme activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Escherichia coli
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Show AA Sequence (130 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.197)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)