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Information on EC 2.3.1.184 - acyl-homoserine-lactone synthase and Organism(s) Pseudomonas aeruginosa

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IUBMB Comments
Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signalling which, in turn, initiates the expression of particular virulence genes . N-(3-Oxohexanoyl)-[acyl-carrier protein] and hexanoyl-[acyl-carrier protein] are the best substrates . The fatty-acyl substrate is derived from fatty-acid biosynthesis through acyl-[acyl-carrier protein] rather than from fatty-acid degradation through acyl-CoA . S-Adenosyl-L-methionine cannot be replaced by methionine, S-adenosylhomocysteine, homoserine or homoserine lactone .
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Select one or more organisms in this record: ?
This record set is specific for:
Pseudomonas aeruginosa
Word Map
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
Synonyms
3-oxo-C12-HSL synthase, AbaI, ACII, acyl homoserine lactone synthase, acyl-homoserine lactone synthase, acyl-homoserine-lactone synthase, acyl-homoserinelactone synthase, acyl-HSL synthase, acylhomoserine lactone synthase, AHL synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-oxo-C12-HSL synthase
-
acyl homoserine lactone synthase
-
-
acyl-homoserinelactone synthase
-
-
acylhomoserine lactone synthase
autoinducer synthesis protein rhlI
-
LasI synthase
-
-
additional information
-
the enzyme belongs to the LuxI family
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
show the reaction diagram
SYSTEMATIC NAME
IUBMB Comments
acyl-[acyl-carrier protein]:S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing)
Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signalling which, in turn, initiates the expression of particular virulence genes [5]. N-(3-Oxohexanoyl)-[acyl-carrier protein] and hexanoyl-[acyl-carrier protein] are the best substrates [1]. The fatty-acyl substrate is derived from fatty-acid biosynthesis through acyl-[acyl-carrier protein] rather than from fatty-acid degradation through acyl-CoA [1]. S-Adenosyl-L-methionine cannot be replaced by methionine, S-adenosylhomocysteine, homoserine or homoserine lactone [1].
CAS REGISTRY NUMBER
COMMENTARY hide
176023-66-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
show the reaction diagram
-
-
-
-
?
3-oxododecanoyl-[acyl carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
-
-
-
ir
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-L-homoserine lactone + [acyl-carrier protein] + S-methyl-5'-thioadenosine
show the reaction diagram
-
-
-
-
?
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
show the reaction diagram
-
LasI
-
-
?
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine
an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
show the reaction diagram
-
-
-
-
?
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
N-butanoyl-L-homoserine lactone + [acyl-carrier protein] + S-methyl-5'-thioadenosine
show the reaction diagram
-
-
-
-
?
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + butanoyl-L-homoserine lactone
show the reaction diagram
-
RhlI
-
-
?
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
-
production is only 1/15 of N-butanoyl homoserine lactone
-
-
ir
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
-
substrate of LasI
-
-
?
S-adenosyl-L-methionine + butyryl-CoA
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
show the reaction diagram
S-adenosyl-L-methionine + butyryl-S-adenosyl-L-methionine
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + S-adenosyl-L-methionine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1]
show the reaction diagram
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2]
show the reaction diagram
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3]
show the reaction diagram
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
show the reaction diagram
-
-
-
-
?
3-oxododecanoyl-[acyl carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
-
-
-
ir
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
show the reaction diagram
-
LasI
-
-
?
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine
an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
show the reaction diagram
-
-
-
-
?
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + butanoyl-L-homoserine lactone
show the reaction diagram
-
RhlI
-
-
?
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
show the reaction diagram
-
production is only 1/15 of N-butanoyl homoserine lactone
-
-
ir
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
-
substrate of LasI
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(4-cyclopentyl-1H-1,2,3-triazol-1-yl)-N-[(3S)-2-oxotetrahydrofuran-3-yl]acetamide
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IC50 value for growth of Pseudomonas aeruginosa 0.610 mM
2-(4-hexyl-1H-1,2,3-triazol-1-yl)-N-[(3S)-2-oxotetrahydrofuran-3-yl]acetamide
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IC50 value for growth of Pseudomonas aeruginosa 0.425 mM
3-[4-(3-formylphenyl)-1H-1,2,3-triazol-1-yl]-N-[(3S)-2-oxotetrahydrofuran-3-yl]propanamide
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IC50 value for growth of Pseudomonas aeruginosa 0.381 mM
5'-methylthioadenosine
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67% inhibition at 0.05 mM, 91% inhibition at 0.5 mM
butyryl-S-adenosyl-L-methionine
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24% inhibition at 0.05 mM, 65% inhibition at 0.5 mM
S-Adenosyl-D-homocysteine
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43% inhibition at 0.05 mM, 88% inhibition at 0.5 mM
S-adenosyl-L-cysteine
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77% inhibition at 0.05 mM, 97% inhibition at 0.5 mM
S-adenosyl-L-homocysteine
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62% inhibition at 0.05 mM, 91% inhibition at 0.5 mM
sinefungin
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58% inhibition at 0.1 mM
Tannic acid
efficiently inhibits N-acylhomoserine lactone production by isoform RhlI, specifically target short-chain N-acylhomoserine lactone synthase RhlI
trans-cinnamaldehyde
efficiently inhibits N-acylhomoserine lactone production by isoform RhlI, specifically target short-chain N-acylhomoserine lactone synthase RhlI. trans-Cinnamaldehyde reduces the quorum-sensing-regulated pyocyanin production up to 42%; molecular docking ananlysis, mainly interacts with substrate binding sites. trans-Cinnamaldehyde is locked deeply into the binding site and forms hydrophobic and Pi-Pi interactions with surrounding residues Phe27, Trp33 and Phe105 and one hydrogen bond with Arg30
[Acyl-carrier-protein]
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holo-[acyl-carrier-protein], 2% inhibition at 0.05 mM, 55% inhibition at 0.5 mM
additional information
-
no inhibition by N-butyryl-L-homoserine-1,4-lactone, CoA, NADH, L-methionine, L-homocysteine, L-homoserine-1,4-lactone, pantothenate, L-homoserine, butyric acid, cerulenin, ATP, ADP, 4-hydroxybutyrate, butyryl-CoA, and apo-ACP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.2 - 0.23
butyryl-CoA
0.038
butyryl-S-adenosyl-L-methionine
-
pH 7.8, 37°C, recombinant RhlI
0.0074
butyryl-[acyl-carrier protein 1]
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pH 8.8, 25°C, recombinant enzyme
0.0059
butyryl-[acyl-carrier protein 2]
-
pH 8.8, 25°C, recombinant enzyme
0.283
butyryl-[acyl-carrier protein 3]
-
pH 8.8, 25°C, recombinant enzyme
0.006
butyryl-[acyl-carrier protein]
-
pH 7.8, 37°C, recombinant RhlI
0.008
hexanoyl-[acyl-carrier protein]
-
pH 7.8, 37°C, recombinant RhlI
0.043
octanoyl-[acyl-carrier protein]
-
pH 7.8, 37°C, recombinant RhlI
0.014
S-adenosyl-L-methionine
-
pH 7.8, 37°C, recombinant RhlI
additional information
additional information
-
reaction kinetics of recombinant RhlI
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05
butyryl-CoA
-
pH 8.8, 25°C, recombinant enzyme
0.35
butyryl-[acyl-carrier protein 1]
-
pH 8.8, 25°C, recombinant enzyme
0.46
butyryl-[acyl-carrier protein 2]
-
pH 8.8, 25°C, recombinant enzyme
0.026
butyryl-[acyl-carrier protein 3]
-
pH 8.8, 25°C, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0021
-
purified recombinant RhlI
additional information
-
activities of mutant LasI enzymes
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pH-profile
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
additional information
-
the LasR protein of Pseudomonas aeruginosa is a central component of a regulatory web that controls the expression of hundreds of genes, some of which play direct roles in disease, molecular mechanism of action of LasR as transcription factor, it detects 3-oxododecanoyl-L-homoserine lactone, overview. The so-called orphan receptor QscR, which also detects 3-oxododecanoyl-L-homoserine lactone. The second quorum sensing receptor, RhlR, detects butanoyl-L-homoserine lactone and interacts with its cognate AHL synthase, RhlI. Unlike LuxR, LasR does not detectably release its N-acyl-homoserine lactone. It binds to six LasR-dependent promoters
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
A0A4P0V481_PSEAI
140
0
16263
TrEMBL
-
D2EDP1_PSEAI
201
0
22600
TrEMBL
-
A0A485E6P1_PSEAI
241
0
27306
TrEMBL
-
A0A0M5IIR8_PSEAI
190
0
21332
TrEMBL
-
A0A3M5DDQ0_PSEAI
206
0
23308
TrEMBL
-
A0A0M4BUF6_PSEAI
187
0
20960
TrEMBL
-
A0A0A8RBK6_PSEAI
201
0
22588
TrEMBL
-
A0A0M5IEJ4_PSEAI
189
0
21218
TrEMBL
-
A0A485G8T5_PSEAI
134
0
14927
TrEMBL
-
A0A411F6V3_PSEAI
196
0
22107
TrEMBL
-
L0GAJ0_PSEAI
201
0
22644
TrEMBL
-
A0A0M3Q187_PSEAI
190
0
21317
TrEMBL
-
A9JPX5_PSEAI
186
0
21010
TrEMBL
-
A9JPX3_PSEAI
201
0
22691
TrEMBL
-
A0A0M4C3S8_PSEAI
190
0
21233
TrEMBL
-
A0A0M4BMQ0_PSEAI
192
0
21609
TrEMBL
-
A0A4P0V7V3_PSEAI
77
0
8437
TrEMBL
-
A0A509JEB5_PSEAI
201
0
22557
TrEMBL
-
A0A0M3Q0K5_PSEAI
190
0
21333
TrEMBL
-
A0A0A8RK20_PSEAI
201
0
22675
TrEMBL
-
A0A0M4C1U7_PSEAI
187
0
21001
TrEMBL
-
D2EDP6_PSEAI
201
0
22586
TrEMBL
-
A0A0M4C318_PSEAI
190
0
21319
TrEMBL
-
A0A485F9D9_PSEAI
202
0
22705
TrEMBL
-
A0A0M4BQA3_PSEAI
188
0
21146
TrEMBL
-
A0A1Z1NE37_PSEAI
168
0
18709
TrEMBL
-
A0A411F6U8_PSEAI
201
0
22673
TrEMBL
-
D2EDN9_PSEAI
201
0
22614
TrEMBL
-
A0A485FQL6_PSEAI
117
0
13256
TrEMBL
-
A0A411HE15_PSEAI
185
0
20936
TrEMBL
-
A0A0M4BU37_PSEAI
188
0
21088
TrEMBL
-
A0A6A9K6B4_PSEAI
201
0
22604
TrEMBL
-
A0A2R3IPS8_PSEAI
201
0
22663
TrEMBL
-
A0A6H0QUM6_PSEAI
185
0
21010
TrEMBL
-
A0A411F6V2_PSEAI
201
0
22630
TrEMBL
-
A0A0M3Q188_PSEAI
189
0
21202
TrEMBL
-
B6E4Z4_PSEAI
201
0
22584
TrEMBL
-
A0A2R3J2K8_PSEAI
202
0
22731
TrEMBL
-
A0A2P1E4T7_PSEAI
190
0
21381
TrEMBL
-
A0A0M4BSD8_PSEAI
190
0
21360
TrEMBL
-
A0A411F6V0_PSEAI
200
0
22499
TrEMBL
-
A0A3S0L5B8_PSEAI
202
0
22758
TrEMBL
-
A0A0M4BSF1_PSEAI
188
0
21058
TrEMBL
-
A0A0X8ELE1_PSEAI
180
0
20123
TrEMBL
-
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C126S
site-directed mutagenesis, the mutant shows 81.5% activity compared to the wild-type enzyme
C67S
site-directed mutagenesis, the mutant shows 1.9% activity compared to the wild-type enzyme
C67S/C69S
site-directed mutagenesis, the mutant shows 0.8% activity compared to the wild-type enzyme
C69S
site-directed mutagenesis, the mutant shows 30.4% activity compared to the wild-type enzyme
C89S
site-directed mutagenesis, the mutant shows 63% activity compared to the wild-type enzyme
D48N
site-directed mutagenesis, inactive mutant
D51N
site-directed mutagenesis, nearly inactive mutant
E101K
site-directed mutagenesis, inactive mutant
E144K
site-directed mutagenesis, the mutant shows 43.5% activity compared to the wild-type enzyme
E46K
site-directed mutagenesis, inactive mutant
E7K/F147L/P159E/E182G
-
strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration
E7K/F147L/V201M
-
strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type
F28L
site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme
G159E
site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme
G68D
site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme
G68E
site-directed mutagenesis, inactive mutant
K150E/R154QE
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG
K150Q
-
site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
K150Q/R154Q
-
site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R104C
site-directed mutagenesis, nearly inactive mutant
R104H
site-directed mutagenesis, nearly inactive mutant
R154E
-
site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R154Q
-
site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
R172A
-
site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG
R24W
site-directed mutagenesis, inactive mutant
R71C
site-directed mutagenesis, the mutant shows 0.05% activity compared to the wild-type enzyme
R71H
site-directed mutagenesis, inactive mutant
S103E
site-directed mutagenesis, the mutant shows 5.4% activity compared to the wild-type enzyme
W34G
site-directed mutagenesis, the mutant shows 0.10% activity compared to the wild-type enzyme
W34Y
site-directed mutagenesis, the mutant shows 60% activity compared to the wild-type enzyme
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage
-
recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
-
expression in Escherichia coli
expression of wild-type and mutant enzymes in Escherichia coli
functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins
-
gene lasI, genetic organization and las genetic locus
-
gene rhlI, expression in Escherichia coli strain XL 1-Blue
-
gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3)
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoform LasI increases with increase in biofilm growth
-
expression of isoform RhlI decreases during log phase of biofilm growth
-
RsaLWCS does not show a repressive effect on the promoter of the QS signal synthase gene lasI in Pseudomonas aeruginosa. RsaLWCS formed stable complexes with the promoter of lasI, the gene orthologous to ppuI. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilm formation, two processes relevant for plant growth-promoting activity. RsaL binds close to LasR on the same bidirectional promoter, repressing the transcription of both the lasI and rsaL genes. Molecular mechanism, overview
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
degradation
-
a significant positive correlation is observed between isoform LasI expression and polycyclic aromatic hydrocarbon degradation. Expression of isoform LasI increases with increase in biofilm growth, while the expression of isoform RhlI decreases during log phase of biofilm growth. Degradation of phenanthrene and pyrene by Pseudomonas aeruginosa N6P6 is affected by biofilm growth and LasI expression. The respective phenanthrene degradation for 15, 24, 48, and 72 h old biofilm after 7 days is 21.5, 54.2, 85.6, and 85.7%. The corresponding pyrene degradation is 15, 18.28, 47.56, and 46.48%, respectively, after 7 days
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Raychaudhuri, A.; Jerga, A.; Tipton, P.A.
Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa
Biochemistry
44
2974-2981
2005
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Parsek, M.R.; Schaefer, A.L.; Greenberg, E.P.
Analysis of random and site-directed mutations in rhlI, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase
Mol. Microbiol.
26
301-310
1997
Pseudomonas aeruginosa (P54291), Pseudomonas aeruginosa
Manually annotated by BRENDA team
Gould, T.A.; Schweizer, H.P.; Churchill, M.E.
Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI
Mol. Microbiol.
53
1135-1146
2004
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Parsek, M.R.; Val, D.L.; Hanzelka, B.L.; Cronan, J.E.; Greenberg, E.P.
Acyl homoserine-lactone quorum-sensing signal generation
Proc. Natl. Acad. Sci. USA
96
4360-4365
1999
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-JP1
Manually annotated by BRENDA team
Steindler, L.; Bertani, I.; De Sordi, L.; Schwager, S.; Eberl, L.; Venturi, V.
LasI/R and RhlI/R quorum sensing in a strain of Pseudomonas aeruginosa beneficial to plants
Appl. Environ. Microbiol.
75
5131-5140
2009
Pseudomonas aeruginosa (A9JPX3), Pseudomonas aeruginosa (A9JPX5), Pseudomonas aeruginosa, Pseudomonas aeruginosa PUPa3 (A9JPX3), Pseudomonas aeruginosa PUPa3 (A9JPX5)
Manually annotated by BRENDA team
Kambam, P.K.; Eriksen, D.T.; Lajoie, J.; Sayut, D.J.; Sun, L.
Altering the substrate specificity of RhlI by directed evolution
ChemBioChem
10
553-558
2009
Pseudomonas aeruginosa
Manually annotated by BRENDA team
Rampioni, G.; Bertani, I.; Pillai, C.R.; Venturi, V.; Zennaro, E.; Leoni, L.
Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358
Appl. Environ. Microbiol.
78
726-734
2012
Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida WCS358
Manually annotated by BRENDA team
Tsai, C.S.; Winans, S.C.
LuxR-type quorum-sensing regulators that are detached from common scents
Mol. Microbiol.
77
1072-1082
2010
Aliivibrio fischeri, Agrobacterium tumefaciens, Escherichia coli, Pectobacterium carotovorum, Dickeya chrysanthemi, Yersinia pestis, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Pseudomonas syringae, Serratia marcescens, Serratia sp., Yersinia enterocolitica, Pantoea stewartii, Serratia sp. 39006, Serratia marcescens SS-1
Manually annotated by BRENDA team
Mangwani, N.; Kumari, S.; Das, S.
Involvement of quorum sensing genes in biofilm development and degradation of polycyclic aromatic hydrocarbons by a marine bacterium Pseudomonas aeruginosa N6P6
Appl. Microbiol. Biotechnol.
99
10283-10297
2015
Pseudomonas aeruginosa, Pseudomonas aeruginosa N6P6
Manually annotated by BRENDA team
Hansen, M.R.; Jakobsen, T.H.; Bang, C.G.; Cohrt, A.E.; Hansen, C.L.; Clausen, J.W.; Le Quement, S.T.; Tolker-Nielsen, T.; Givskov, M.; Nielsen, T.E.
Triazole-containing N-acyl homoserine lactones targeting the quorum sensing system in Pseudomonas aeruginosa
Bioorg. Med. Chem.
23
1638-1650
2015
Escherichia coli, Pseudomonas aeruginosa
Manually annotated by BRENDA team
Chang, C.; Krishnan, T.; Wang, H.; Chen, Y.; Yin, W.; Chong, Y.; Tan, L.; Chong, T.; Chan, K.
Non-antibiotic quorum sensing inhibitors acting against N-acyl homoserine lactone synthase as druggable target
Sci. Rep.
4
7245
2014
Pseudomonas aeruginosa (P33883), Pseudomonas aeruginosa (P54291), Pseudomonas aeruginosa
Manually annotated by BRENDA team
Yang, Y.; Zhou, M.; Hardwidge, P.; Cui, H.; Zhu, G.
Isolation and characterization of N-acyl homoserine lactone-producing bacteria from cattle rumen and swine intestines
Front. Cell. Infect. Microbiol.
8
155
2018
Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas aeruginosa YZ1, Aeromonas hydrophila YZ2
Manually annotated by BRENDA team
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