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Information on EC 2.3.1.184 - acyl-homoserine-lactone synthase and Organism(s) Pseudomonas aeruginosa
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2.3.1.184 acyl-homoserine-lactone synthaseIUBMB Comments
Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signalling which, in turn, initiates the expression of particular virulence genes . N-(3-Oxohexanoyl)-[acyl-carrier protein] and hexanoyl-[acyl-carrier protein] are the best substrates . The fatty-acyl substrate is derived from fatty-acid biosynthesis through acyl-[acyl-carrier protein] rather than from fatty-acid degradation through acyl-CoA . S-Adenosyl-L-methionine cannot be replaced by methionine, S-adenosylhomocysteine, homoserine or homoserine lactone .
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Word Map
- 2.3.1.184
-
quorum
-
quorum-sensing
-
n-acylhomoserine
-
density-dependent
- fischeri
-
acyl-hsls
-
ahl-dependent
-
cell-density-dependent
-
qs-regulated
-
n-acyl-l-homoserine
-
luxi-type
-
acyl-acyl
- n-3-oxohexanoyl-l-homoserine
-
ahl-producing
-
ahl-mediated
-
c8-hsl
- drug development
- n-octanoyl-l-homoserine
- medicine
- degradation
The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
ahl synthase, autoinducer synthase, lasi/r, acyl-hsl synthase, rhli/r, luxi protein, acyl homoserine lactone synthase, n-acyl-homoserine lactone synthase, ahsl synthase, acylhomoserine lactone synthase, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
acylhomoserine lactone synthase
LasI
RhlI
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
substrate binding structure
-
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
RhlI contains no active site cysteine, residues R24, F28, W34, E46, D48, D51, G68, C67, R71, E101, S103, and R104 are critical for catalytic activity
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
RhlI shows a sequential, ordered ping-pong reaction mechanism with S-adenosyl-L-methionine binding first
-
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
structures of substrate, product, and transition state, overview
-
PATHWAY SOURCE
PATHWAYS
MetaCyc
SYSTEMATIC NAME
IUBMB Comments
acyl-[acyl-carrier protein]:S-adenosyl-L-methionine acyltranserase (lactone-forming, methylthioadenosine-releasing)
Acyl-homoserine lactones (AHLs) are produced by a number of bacterial species and are used by them to regulate the expression of virulence genes in a process known as quorum-sensing. Each bacterial cell has a basal level of AHL and, once the population density reaches a critical level, it triggers AHL-signalling which, in turn, initiates the expression of particular virulence genes [5]. N-(3-Oxohexanoyl)-[acyl-carrier protein] and hexanoyl-[acyl-carrier protein] are the best substrates [1]. The fatty-acyl substrate is derived from fatty-acid biosynthesis through acyl-[acyl-carrier protein] rather than from fatty-acid degradation through acyl-CoA [1]. S-Adenosyl-L-methionine cannot be replaced by methionine, S-adenosylhomocysteine, homoserine or homoserine lactone [1].
CAS REGISTRY NUMBER
COMMENTARY
176023-66-8
-
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
-
-
?
3-oxododecanoyl-[acyl carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-L-homoserine lactone + [acyl-carrier protein] + S-methyl-5'-thioadenosine
-
-
-
?
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
LasI
-
?
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine
an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
-
-
-
?
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
N-butanoyl-L-homoserine lactone + [acyl-carrier protein] + S-methyl-5'-thioadenosine
-
-
-
?
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + butanoyl-L-homoserine lactone
-
RhlI
-
?
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
production is only 1/15 of N-butanoyl homoserine lactone
-
ir
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
substrate of LasI
-
?
S-adenosyl-L-methionine + butyryl-CoA
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
S-adenosyl-L-methionine + butyryl-S-adenosyl-L-methionine
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + S-adenosyl-L-methionine
-
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 1]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 1]
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 2]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 2]
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein 3]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein 3]
-
substrate of RhlI, three different [acyl-carrier-protein] isozymes from Pseudomonas aeruginosa serve as substrates, very low activity with ACP3
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
S-adenosyl-L-methionine + hexanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-hexanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
-
-
?
S-adenosyl-L-methionine + octanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-octanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
-
-
?
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
ir
S-adenosyl-L-methionine + butyryl-CoA
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
-
-
-
?
S-adenosyl-L-methionine + butyryl-CoA
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + CoA
-
substrate of RhlI, very low activity
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity
-
?
additional information
?
-
-
LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa
-
?
additional information
?
-
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview
-
?
additional information
?
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator
-
?
additional information
?
-
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator
-
?
additional information
?
-
-
substrate specificity of RhlI, no activity with butyrate, hexanoyl-CoA, and decanoyl-[acyl-carrier-protein], no activity with S-adenosyl-L-homocysteine, S-adenosyl-L-cysteine, L-homoserine lactone, L-homocystein, L-homoserine, and L-methionine as substrates
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-oxo-dodecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
-
-
-
?
3-oxododecanoyl-[acyl carrier protein] + S-adenosyl-L-methionine
N-(3-oxododecanoyl)-homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
3-oxododecanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + N-3-oxododecanoyl-L-homoserine lactone
-
LasI
-
-
?
an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine
an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone
-
-
-
-
?
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
butanoyl-[acyl-carrier protein] + S-adenosyl-L-methionine
[acyl-carrier protein] + S-methyl-5'-thioadenosine + butanoyl-L-homoserine lactone
-
RhlI
-
-
?
hexanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-hexanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
production is only 1/15 of N-butanoyl homoserine lactone
-
-
ir
S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein]
5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
substrate of LasI
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
-
ir
butanoyl [acyl carrier protein] + S-adenosyl-L-methionine
N-butanoyl homoserine lactone + [acyl carrier protein] + 5'-methyl-thioadenosine
-
-
-
ir
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
-
-
?
S-adenosyl-L-methionine + butyryl-[acyl-carrier protein]
5'-methylthioadenosine + N-butyryl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
substrate of RhlI, synthesis of a quorum-sensing molecule involved in the regulation of many processes involving the bacterial virulence and infectivity
-
-
?
additional information
?
-
-
LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa
-
-
?
additional information
?
-
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules which regulates the virulence genes, N-acylhomoserine lactones are autoinducers, the enzyme acts as a quorum-sensing signal generator, mechanism, overview
-
-
?
additional information
?
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator
-
-
?
additional information
?
-
-
the enzyme is involved in quorum-sensing signaling by synthesizing the signaling molecules, the autoinducers N-acylhomoserine lactones, the enzyme acts as a quorum-sensing signal generator
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-(4-cyclopentyl-1H-1,2,3-triazol-1-yl)-N-[(3S)-2-oxotetrahydrofuran-3-yl]acetamide
-
IC50 value for growth of Pseudomonas aeruginosa 0.610 mM
2-(4-hexyl-1H-1,2,3-triazol-1-yl)-N-[(3S)-2-oxotetrahydrofuran-3-yl]acetamide
-
IC50 value for growth of Pseudomonas aeruginosa 0.425 mM
3-[4-(3-formylphenyl)-1H-1,2,3-triazol-1-yl]-N-[(3S)-2-oxotetrahydrofuran-3-yl]propanamide
-
IC50 value for growth of Pseudomonas aeruginosa 0.381 mM
5'-methylthioadenosine
-
67% inhibition at 0.05 mM, 91% inhibition at 0.5 mM
butyryl-S-adenosyl-L-methionine
-
24% inhibition at 0.05 mM, 65% inhibition at 0.5 mM
S-Adenosyl-D-homocysteine
-
43% inhibition at 0.05 mM, 88% inhibition at 0.5 mM
S-adenosyl-L-cysteine
-
77% inhibition at 0.05 mM, 97% inhibition at 0.5 mM
S-adenosyl-L-homocysteine
-
62% inhibition at 0.05 mM, 91% inhibition at 0.5 mM
Tannic acid
efficiently inhibits N-acylhomoserine lactone production by isoform RhlI, specifically target short-chain N-acylhomoserine lactone synthase RhlI
trans-cinnamaldehyde
efficiently inhibits N-acylhomoserine lactone production by isoform RhlI, specifically target short-chain N-acylhomoserine lactone synthase RhlI. trans-Cinnamaldehyde reduces the quorum-sensing-regulated pyocyanin production up to 42%; molecular docking ananlysis, mainly interacts with substrate binding sites. trans-Cinnamaldehyde is locked deeply into the binding site and forms hydrophobic and Pi-Pi interactions with surrounding residues Phe27, Trp33 and Phe105 and one hydrogen bond with Arg30
[Acyl-carrier-protein]
-
holo-[acyl-carrier-protein], 2% inhibition at 0.05 mM, 55% inhibition at 0.5 mM
additional information
-
no inhibition by N-butyryl-L-homoserine-1,4-lactone, CoA, NADH, L-methionine, L-homocysteine, L-homoserine-1,4-lactone, pantothenate, L-homoserine, butyric acid, cerulenin, ATP, ADP, 4-hydroxybutyrate, butyryl-CoA, and apo-ACP
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.038
butyryl-S-adenosyl-L-methionine
-
pH 7.8, 37°C, recombinant RhlI
0.0074
butyryl-[acyl-carrier protein 1]
-
pH 8.8, 25°C, recombinant enzyme
0.0059
butyryl-[acyl-carrier protein 2]
-
pH 8.8, 25°C, recombinant enzyme
0.283
butyryl-[acyl-carrier protein 3]
-
pH 8.8, 25°C, recombinant enzyme
0.008
hexanoyl-[acyl-carrier protein]
-
pH 7.8, 37°C, recombinant RhlI
0.043
octanoyl-[acyl-carrier protein]
-
pH 7.8, 37°C, recombinant RhlI
additional information
additional information
-
reaction kinetics of recombinant RhlI
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.35
butyryl-[acyl-carrier protein 1]
-
pH 8.8, 25°C, recombinant enzyme
0.46
butyryl-[acyl-carrier protein 2]
-
pH 8.8, 25°C, recombinant enzyme
0.026
butyryl-[acyl-carrier protein 3]
-
pH 8.8, 25°C, recombinant enzyme
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rice rhizosphere isolate, and knockout mutations
SwissProt
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
additional information
-
the LasR protein of Pseudomonas aeruginosa is a central component of a regulatory web that controls the expression of hundreds of genes, some of which play direct roles in disease, molecular mechanism of action of LasR as transcription factor, it detects 3-oxododecanoyl-L-homoserine lactone, overview. The so-called orphan receptor QscR, which also detects 3-oxododecanoyl-L-homoserine lactone. The second quorum sensing receptor, RhlR, detects butanoyl-L-homoserine lactone and interacts with its cognate AHL synthase, RhlI. Unlike LuxR, LasR does not detectably release its N-acyl-homoserine lactone. It binds to six LasR-dependent promoters
physiological function
quorum sensing regulates motility and lipase, and antifungal activities
physiological function
quorum sensing regulates motility and lipase, protease, and antifungal activities
physiological function
-
chemical communication within populations of bacteria enable them to estimate their population density, a process sometimes referred to as quorum sensing. Signalling among Proteobacteria often involves N-acyl-homoserine lactones, which have identical polar head groups and a variety of hydrophobic acyl groups that differ in length, oxidation, and desaturation. AHL signal molecules are often referred to as autoinducers. They are synthesized by LuxI-type AHL synthases. Most LuxR-type receptors, i.e. LuxR, LasR, and TraR, require N-acyl-homoserine lactones for function and in at least some cases, N-acyl-homoserine lactones are required for protein folding and protease resistance. The LasR/LasI system stimulates production of the RhlI/RhlR system, causing the two Pseudomonas aeruginosa quorum-sensing circuits to initiate sequentially
physiological function
-
quorum sensing systems rely on a signal receptor and a synthase producing N-acyl-homoserine lactone(s) as the signal molecule(s). The rsaL gene, located between the signal receptor and synthase genes, encodes a repressor limiting signal synthase expression and hence signal molecule production, molecular mechannism, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C126S
site-directed mutagenesis, the mutant shows 81.5% activity compared to the wild-type enzyme
C67S
site-directed mutagenesis, the mutant shows 1.9% activity compared to the wild-type enzyme
C67S/C69S
site-directed mutagenesis, the mutant shows 0.8% activity compared to the wild-type enzyme
C69S
site-directed mutagenesis, the mutant shows 30.4% activity compared to the wild-type enzyme
C89S
site-directed mutagenesis, the mutant shows 63% activity compared to the wild-type enzyme
E144K
site-directed mutagenesis, the mutant shows 43.5% activity compared to the wild-type enzyme
E7K/F147L/P159E/E182G
-
strain R2: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type and yield a hexanoyl homoserine lactone level comparable to the butanoyl homoserine lactone concentration
E7K/F147L/V201M
-
strain R1: cells produce twicefold amount of butanoyl homoserine lactone compared to wild type
F28L
site-directed mutagenesis, the mutant shows 0.18% activity compared to the wild-type enzyme
G159E
site-directed mutagenesis, the mutant shows 44.6% activity compared to the wild-type enzyme
G68D
site-directed mutagenesis, the mutant shows 0.075% activity compared to the wild-type enzyme
K150E/R154QE
-
site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG
K150Q
-
site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
K150Q/R154Q
-
site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R154E
-
site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG
R154Q
-
site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG
R172A
-
site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG
R71C
site-directed mutagenesis, the mutant shows 0.05% activity compared to the wild-type enzyme
S103E
site-directed mutagenesis, the mutant shows 5.4% activity compared to the wild-type enzyme
W34G
site-directed mutagenesis, the mutant shows 0.10% activity compared to the wild-type enzyme
W34Y
site-directed mutagenesis, the mutant shows 60% activity compared to the wild-type enzyme
additional information
additional information
screening of diverse mutants constructrd by random mutagenesis, overview
additional information
-
screening of diverse mutants constructrd by random mutagenesis, overview
additional information
strains lacking RhlI/R or LasI/R are created
additional information
strains lacking RhlI/R or LasI/R are created
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage
-
recombinant maltose binding protein-fusion enzyme RhlI from Escherichia coli strain XL 1-Blue by amylose affinity chromatography, recombinant His-tagged RhlI from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant RhlI 95fold from Escherichia coli strain XL 1-Blue by anion and cation exchange chromatography and gel filtration
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins
-
gene rhlI, overexpression in Escherichia coli strain XL 1-Blue as maltose binding protein-fusion protein, overexpression of His-tagged RhlI in Escherichia coli strain BL21(DE3)
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression of isoform LasI increases with increase in biofilm growth
-
expression of isoform RhlI decreases during log phase of biofilm growth
-
RsaLWCS does not show a repressive effect on the promoter of the QS signal synthase gene lasI in Pseudomonas aeruginosa. RsaLWCS formed stable complexes with the promoter of lasI, the gene orthologous to ppuI. RsaLWCS regulates siderophore-mediated growth limitation of plant pathogens and biofilm formation, two processes relevant for plant growth-promoting activity. RsaL binds close to LasR on the same bidirectional promoter, repressing the transcription of both the lasI and rsaL genes. Molecular mechanism, overview
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
degradation
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a significant positive correlation is observed between isoform LasI expression and polycyclic aromatic hydrocarbon degradation. Expression of isoform LasI increases with increase in biofilm growth, while the expression of isoform RhlI decreases during log phase of biofilm growth. Degradation of phenanthrene and pyrene by Pseudomonas aeruginosa N6P6 is affected by biofilm growth and LasI expression. The respective phenanthrene degradation for 15, 24, 48, and 72 h old biofilm after 7 days is 21.5, 54.2, 85.6, and 85.7%. The corresponding pyrene degradation is 15, 18.28, 47.56, and 46.48%, respectively, after 7 days
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Raychaudhuri, A.; Jerga, A.; Tipton, P.A.
Chemical mechanism and substrate specificity of RhlI, an acylhomoserine lactone synthase from Pseudomonas aeruginosa
Biochemistry
44
2974-2981
2005
Pseudomonas aeruginosa
Parsek, M.R.; Schaefer, A.L.; Greenberg, E.P.
Analysis of random and site-directed mutations in rhlI, a Pseudomonas aeruginosa gene encoding an acylhomoserine lactone synthase
Mol. Microbiol.
26
301-310
1997
Pseudomonas aeruginosa (P54291), Pseudomonas aeruginosa
Gould, T.A.; Schweizer, H.P.; Churchill, M.E.
Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI
Mol. Microbiol.
53
1135-1146
2004
Pseudomonas aeruginosa
Parsek, M.R.; Val, D.L.; Hanzelka, B.L.; Cronan, J.E.; Greenberg, E.P.
Acyl homoserine-lactone quorum-sensing signal generation
Proc. Natl. Acad. Sci. USA
96
4360-4365
1999
Pseudomonas aeruginosa, Pseudomonas aeruginosa PAO-JP1
Steindler, L.; Bertani, I.; De Sordi, L.; Schwager, S.; Eberl, L.; Venturi, V.
LasI/R and RhlI/R quorum sensing in a strain of Pseudomonas aeruginosa beneficial to plants
Appl. Environ. Microbiol.
75
5131-5140
2009
Pseudomonas aeruginosa (A9JPX3), Pseudomonas aeruginosa (A9JPX5), Pseudomonas aeruginosa, Pseudomonas aeruginosa PUPa3 (A9JPX3), Pseudomonas aeruginosa PUPa3 (A9JPX5)
Kambam, P.K.; Eriksen, D.T.; Lajoie, J.; Sayut, D.J.; Sun, L.
Altering the substrate specificity of RhlI by directed evolution
ChemBioChem
10
553-558
2009
Pseudomonas aeruginosa
Rampioni, G.; Bertani, I.; Pillai, C.R.; Venturi, V.; Zennaro, E.; Leoni, L.
Functional characterization of the quorum sensing regulator RsaL in the plant-beneficial strain Pseudomonas putida WCS358
Appl. Environ. Microbiol.
78
726-734
2012
Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida WCS358
Tsai, C.S.; Winans, S.C.
LuxR-type quorum-sensing regulators that are detached from common scents
Mol. Microbiol.
77
1072-1082
2010
Aliivibrio fischeri, Agrobacterium tumefaciens, Escherichia coli, Pectobacterium carotovorum, Dickeya chrysanthemi, Yersinia pestis, Yersinia pseudotuberculosis, Pseudomonas aeruginosa, Pseudomonas syringae, Serratia marcescens, Serratia sp., Yersinia enterocolitica, Pantoea stewartii, Serratia sp. 39006, Serratia marcescens SS-1
Mangwani, N.; Kumari, S.; Das, S.
Involvement of quorum sensing genes in biofilm development and degradation of polycyclic aromatic hydrocarbons by a marine bacterium Pseudomonas aeruginosa N6P6
Appl. Microbiol. Biotechnol.
99
10283-10297
2015
Pseudomonas aeruginosa, Pseudomonas aeruginosa N6P6
Hansen, M.R.; Jakobsen, T.H.; Bang, C.G.; Cohrt, A.E.; Hansen, C.L.; Clausen, J.W.; Le Quement, S.T.; Tolker-Nielsen, T.; Givskov, M.; Nielsen, T.E.
Triazole-containing N-acyl homoserine lactones targeting the quorum sensing system in Pseudomonas aeruginosa
Bioorg. Med. Chem.
23
1638-1650
2015
Escherichia coli, Pseudomonas aeruginosa
Chang, C.; Krishnan, T.; Wang, H.; Chen, Y.; Yin, W.; Chong, Y.; Tan, L.; Chong, T.; Chan, K.
Non-antibiotic quorum sensing inhibitors acting against N-acyl homoserine lactone synthase as druggable target
Sci. Rep.
4
7245
2014
Pseudomonas aeruginosa (P33883), Pseudomonas aeruginosa (P54291), Pseudomonas aeruginosa
Yang, Y.; Zhou, M.; Hardwidge, P.; Cui, H.; Zhu, G.
Isolation and characterization of N-acyl homoserine lactone-producing bacteria from cattle rumen and swine intestines
Front. Cell. Infect. Microbiol.
8
155
2018
Aeromonas hydrophila, Pseudomonas aeruginosa, Pseudomonas aeruginosa YZ1, Aeromonas hydrophila YZ2
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