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Information on EC 2.3.1.183 - phosphinothricin acetyltransferase and Organism(s) Pseudomonas aeruginosa and UniProt Accession Q9HUU7

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IUBMB Comments
The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, glutamine synthetase, a key enzyme of nitrogen metabolism in plants .
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This record set is specific for:
Pseudomonas aeruginosa
UNIPROT: Q9HUU7
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The taxonomic range for the selected organisms is: Pseudomonas aeruginosa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
phosphinothricin acetyltransferase, phosphinothricin-n-acetyltransferase, phosphinothricin n-acetyltransferase, ppt acetyltransferase, gk2920, gk0593, dr_1182, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphinothricin acetyltransferase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:phosphinothricin N-acetyltransferase
The substrate phosphinothricin is used as a nonselective herbicide and is a potent inhibitor of EC 6.3.1.2, glutamine synthetase, a key enzyme of nitrogen metabolism in plants [2].
CAS REGISTRY NUMBER
COMMENTARY hide
111069-93-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + L-methionine sulfone
?
show the reaction diagram
-
-
-
?
acetyl-CoA + L-methionine sulfoximine
?
show the reaction diagram
enzyme has no activity towards phosphinothricin
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
acetyl-CoA
apparent KM
1.3
L-methionine sulfone
apparent KM
1.3
L-methionine sulfoximine
apparent KM
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
610
L-methionine sulfone
apparent Kcat
505
L-methionine sulfoximine
apparent Kcat
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.2
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
putative uncharacterized protein
UniProt
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a high-resolution, 1.55 A, crystal structure of pita in complex with L-methionine sulfoximine is solved
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Depp4
mutant, in which pita is inactivated through an in-frame deletion
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into the vector pET24a for expression in Escherichia coli BL21DE3 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Davies, A.M.; Tata, R.; Beavil, R.L.; Sutton, B.J.; Brown, P.R.
l-Methionine sulfoximine, but not phosphinothricin, is a substrate for an acetyltransferase (gene PA4866) from Pseudomonas aeruginosa: structural and functional studies
Biochemistry
46
1829-1839
2007
Pseudomonas aeruginosa (Q9HUU7), Pseudomonas aeruginosa
Manually annotated by BRENDA team