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Information on EC 2.3.1.171 - anthocyanin 6''-O-malonyltransferase and Organism(s) Dahlia pinnata and UniProt Accession Q8GSN8

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IUBMB Comments
Acts on pelargonidin 3-O-glucoside in dahlia (Dahlia variabilis), delphinidin 3-O-glucoside, and on cyanidin 3-O-glucoside in transgenic petunia (Petunia hybrida).
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This record set is specific for:
Dahlia pinnata
UNIPROT: Q8GSN8
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The taxonomic range for the selected organisms is: Dahlia pinnata
The enzyme appears in selected viruses and cellular organisms
Synonyms
dv3mat, dm3mat1, anthocyanin malonyltransferase, protein grmzm2g387394, anthocyanidin malonyltransferase, malonyl-coenzyme a:anthocyanidin 3-o-glucoside-6"-o-malonyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
anthocyanin malonyltransferase
-
malonyl-CoA: anthocyanidin 3-O-glucoside-6''-O-malonyltransferase
-
malonyl-coenzyme A:anthocyanidin 3-O-glucoside-6"-O-malonyltransferase
-
anthocyanidin 3-glucoside malonyltransferase
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
malonyl-CoA:anthocyanidin-3-O-beta-D-glucoside 6''-O-malonyltransferase
Acts on pelargonidin 3-O-glucoside in dahlia (Dahlia variabilis), delphinidin 3-O-glucoside, and on cyanidin 3-O-glucoside in transgenic petunia (Petunia hybrida).
CAS REGISTRY NUMBER
COMMENTARY hide
111070-07-6
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
-
-
?
malonyl-CoA + cyanidin 3-O-beta-D-glucoside
CoA + cyanidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
-
-
-
?
malonyl-CoA + delphinidin 3-O-beta-D-glucoside
CoA + delphinidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
121% of the activity with cyanidin 3-O-glucoside
-
-
?
malonyl-CoA + malvidin 3-O-beta-D-glucoside
CoA + malvidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
123% of the activity with cyanidin 3-O-glucoside
-
-
?
malonyl-CoA + pelargonidin 3-O-beta-D-glucoside
CoA + pelargonidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
92% of the activity with cyanidin 3-O-glucoside
-
-
?
malonyl-CoA + peonidin 3-O-beta-D-glucoside
CoA + peonidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
96% of the activity with cyanidin 3-O-glucoside
-
-
?
malonyl-CoA + petunidin 3-O-beta-D-glucoside
CoA + petunidin 3-O-(6-O-malonyl-beta-D-glucoside)
show the reaction diagram
-
110% of the activity with cyanidin 3-O-glucoside
-
-
?
succinyl-CoA + cyanidin 3-O-beta-D-glucoside
CoA + cyanidin 3-O-(6-O-succinyl-beta-D-glucoside)
show the reaction diagram
-
7% of the activity with malonyl-CoA
-
-
?
additional information
?
-
-
no reaction with cyanidin 3,5-diglucoside and pelargonidin 3,5-diglucoside
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CoA
0.1 mM, 13% inhibition
Cu2+
0.1 mM, 20°C, 20 min, complete inhibition
Fe2+
0.1 mM, 64% inhibition
Hg2+
0.1 mM, 65% inhibition
NEM
5 mM, 20°C, 20 min, complete inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0188
malonyl-CoA
pH 7.0, 30°C
0.0467
pelargonidin 3-O-beta-D-glucoside
pH 7.0, 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.3
malonyl-CoA
pH 7.0, 30°C, value is obtained by measurement of formation of pelargonidin 3-O-(6'-O-malonyl-beta-D-glucoside)
7.3
pelargonidin 3-O-beta-D-glucoside
pH 7.0, 30°C, value is obtained by measurement of formation of pelargonidin 3-O-(6'-O-malonyl-beta-D-glucoside)
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4 - 9.7
-
50% of maximal activity at pH 6.4 and at pH 9.7
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
3MAT_DAHPI
460
0
51302
Swiss-Prot
other Location (Reliability: 2)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biotechnology
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
medicine
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
synthesis
production of anthocyanins to use their anti-oxidative, anti-inflammatory, anti-cancer, anti-obesity, anti-diabetic, and cardioprotective properties
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as recombinant protein in Escherichia coli, together with other enzymes of the anthocyanidin 3-O-glucoside biosynthetic pathway
expression in Escherichia coli. Creation of transgenic Petunia hybrida harboring Dv3MaT cDNA
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
the gene for anthocyanin malonyltransferase should be useful in the stabilization of anthocyanin pigments, which enable flower color modification in transgenic plants
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamaguchi, M.A.; Oshida, N.; Nakayama, M.; Koshioka, M.; Yamaguchi, Y.; Ino, I.
Anthocyanidin 3-glucoside malonyltransferase from Dahlia variabilis
Phytochemistry
52
15-18
1999
Dahlia pinnata
-
Manually annotated by BRENDA team
Suzuki, H.; Nakayama, T.; Yonekura-Sakakibara, K.; Fukui, Y.; Nakamura, N.; Yamaguchi, M.A.; Tanaka, Y.; Kusumi, T.; Nishino, T.
cDNA cloning, heterologous expressions, and functional characterization of malonyl-coenzyme A:anthocyanidin 3-O-glucoside-6"-O-malonyltransferase from dahlia flowers
Plant Physiol.
130
2142-2151
2002
Dahlia pinnata (Q8GSN8)
Manually annotated by BRENDA team
Yan, Y.; Li, Z.; Koffas, M.A.
High-yield anthocyanin biosynthesis in engineered Escherichia coli
Biotechnol. Bioeng.
100
126-140
2008
Dahlia pinnata (Q8GSN8)
Manually annotated by BRENDA team