Information on EC 2.3.1.162 - taxadien-5alpha-ol O-acetyltransferase

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The expected taxonomic range for this enzyme is: Taxus

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EC NUMBER
COMMENTARY hide
2.3.1.162
-
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RECOMMENDED NAME
GeneOntology No.
taxadien-5alpha-ol O-acetyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
acetyl-CoA + taxa-4(20),11-dien-5alpha-ol = CoA + taxa-4(20),11-dien-5alpha-yl acetate
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
taxol biosynthesis
-
-
Diterpenoid biosynthesis
-
-
Biosynthesis of secondary metabolites
-
-
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SYSTEMATIC NAME
IUBMB Comments
acetyl-CoA:taxa-4(20),11-dien-5alpha-ol O-acetyltransferase
This is the third enzyme in the biosynthesis of the diterpenoid antineoplastic drug taxol (paclitaxel), which is widely used in the treatment of carcinomas, sarcomas and melanomas.
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CAS REGISTRY NUMBER
COMMENTARY hide
229032-29-5
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
SwissProt
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + farnesol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + geranylgeraniol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + phytol
CoA + ?
show the reaction diagram
-
-
-
-
?
acetyl-CoA + taxa-4(20),11(12)-dien-5alpha-ol
CoA + taxa-4(20),11-dien-5alpha-yl acetate
show the reaction diagram
acetyl-CoA + taxadien-2alpha,5alpha,10beta-triol
CoA + taxadien-10beta-acetoxy-2alpha,5alpha-diol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-2alpha,5alpha,10beta-triol
CoA + taxadien-5alpha-acetoxy-2alpha,10beta-diol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-2alpha,5alpha-diol
CoA + taxadien-5alpha-acetoxy-2alpha-ol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha,9alpha,10beta,13alpha-tetraol
CoA + taxadien-10beta-acetoxy-5alpha,9alpha,13alpha-triol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha,9alpha,10beta,13alpha-tetraol
CoA + taxadien-13alpha-acetoxy-5alpha,9alpha,10beta-triol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol + H2O
CoA + taxadien-5alpha,13alpha-diacetoxy-9alpha,10beta-diol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol + H2O
CoA + taxadien-5alpha,9alpha,10beta-triacetoxy-13alpha-ol
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadien-5alpha-ol + H2O
CoA + taxadien-5alpha-yl acetate
show the reaction diagram
-
-
-
?
acetyl-CoA + taxadienol
CoA + ?
show the reaction diagram
-
-
-
-
?
additional information
?
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-ethylmaleimide
p-hydroxymercuribenzoate
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0055
acetyl-CoA
0.0042
taxa-4(20),11-dien-5alpha-ol
0.0129 - 0.0415
taxadien-2alpha,5alpha,10beta-triol
0.0239
taxadien-2alpha,5alpha-diol
-
0.0173 - 0.0453
taxadien-5alpha,9alpha,10beta,13alpha-tetraol
0.0835 - 0.2794
taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol
0.005 - 0.0086
taxadien-5alpha-ol
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.77 - 27.92
taxadien-2alpha,5alpha,10beta-triol
52.13
taxadien-2alpha,5alpha-diol
-
11.91 - 15.47
taxadien-5alpha,9alpha,10beta,13alpha-tetraol
145 - 297.9
taxadien-5alpha-acetoxy-9alpha,10beta,13alpha-triol
4.28 - 29.59
taxadien-5alpha-ol
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5 - 10
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
calculation from nucleotide sequence
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong expression
Manually annotated by BRENDA team
weak expression
Manually annotated by BRENDA team
additional information
no expression in fruits
Manually annotated by BRENDA team
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
x * 49000, calculation from nucleotide sequence
49080
-
recombinant enzyme, calculated from amino acid composition
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
800fold purification
-
partially
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
reconstruction of taxoid biosynthesis in Saccharomyces cerevisiae by simultaneous expression of five early pathway steps starting from isoprenoid precursors. Production of taxadiene reaches levels of 0.7 mg/l in YPG media and 1.0 mg/l in selective media
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.162)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)