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Information on EC 2.3.1.15 - glycerol-3-phosphate 1-O-acyltransferase and Organism(s) Homo sapiens and UniProt Accession Q9HCL2

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EC Tree
IUBMB Comments
Acyl-[acyl-carrier protein] can also act as acyl donor. The enzyme acts only on derivatives of fatty acids of chain length larger than C10.
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q9HCL2
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
glycerol-3-phosphate acyltransferase, gpat1, glycerophosphate acyltransferase, sn-glycerol-3-phosphate acyltransferase, gpat2, gpat3, gpat4, agpat6, agpat, gat1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cancer-testicular antigen
-
glycerol-3-phosphate acyltransferase
-
mitochondrial GPAT1
-
mitochondrial GPAT2
-
3-glycerophosphate acyltransferase
-
-
-
-
ACP:sn-glycerol-3-phosphate acyltransferase
-
-
-
-
acyltransferase, glycerol phosphate
-
-
-
-
AGPAT6
alpha-glycerophosphate acyltransferase
-
-
-
-
ER-associated GPAT
-
-
glycerol 3-phosphate acyltransferase
-
-
-
-
glycerol phosphate acyltransferase
-
-
-
-
glycerol phosphate transacylase
-
-
-
-
glycerol-3-phosphate acyltransferase
glycerol-3-phosphate acyltransferase 3
four isoenzymes are identified in mammals
glycerol-3-phosphate acyltransferase 4
four isoenzymes are identified in mammals
glycerophosphate acyltransferase
-
-
-
-
glycerophosphate transacylase
-
-
-
-
GPAM
-
-
GPAT
-
-
GPAT3
GPAT3/AGPAT10
-
GPAT4
microsomal GPAT3
-
microsomal GPAT3/AGPAT10
-
microsomal GPAT4
-
mtGPAT
-
-
Sn-glycerol 3-phosphate acyltransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as acyl donor. The enzyme acts only on derivatives of fatty acids of chain length larger than C10.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-96-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
fatty acyl-CoA + glycerol-3-phosphate
CoA + lysophosphatidic acid
show the reaction diagram
-
-
-
-
?
glycerol 3-phosphate + palmitoyl-CoA
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + glycerol-3-phosphate
CoA + 1-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
lauroyl-CoA + sn-glycerol 3-phosphate
CoA + 1-lauroyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
linoleoyl-CoA + glycerol-3-phosphate
CoA + 1-linoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
oleoyl-CoA + glycerol-3-phosphate
CoA + 1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
palmitoyl-CoA + glycerol-3-phosphate
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
additional information
?
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2-(nonylsulfonamido)benzoic acid
i.e. FSG67a, GPAT inhibitor, has a broad spectrum inhibitory effect on GPAT activity
NEM
mitochondrial GPAT2 is sensitive to NEM
(+-)-2-(2-hydroxy-3-(octylsulfonamido)-cyclopentyl)acetic acid
-
8% inhibition at 40 microg/ml
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclohexanecarboxylic acid
-
36% inhibition at 40 microg/ml
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclopentane carboxylic acid
-
97% inhibition at 40 microg/ml
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclopentanecarboxylic acid
-
3% inhibition at 40 microg/ml
2-(2-phenylethylsulfonamido)benzoic acid
-
-
2-(4-chlorophenylsulfonamido)benzoic acid
-
-
2-(hexadecylsulfonamido)benzoic acid
-
-
2-(methylsulfonamido)benzoic acid
-
-
2-(nonylsulfonamido)benzoic acid
2-(nonylsulfonamido)benzylphosphonic acid
-
-
2-(octylsulfonamido)phenylphosphonic acid
-
-
2-(pentylsulfonamido)benzylphosphonic acid
-
-
2-(phenylmethylsulfonamido)benzoic acid
-
-
2-(phenylsulfonamido)benzoic acid
-
-
2-(tetradecylsulfonamido)benzoic acid
-
-
3-(2-phenylethylsulfonamido)benzoic acid
-
-
3-(4-chlorophenylsulfonamido)benzoic acid
-
-
3-(nonylsulfonamido)benzoic acid
-
-
3-(nonylsulfonamido)benzylphosphonic acid
-
-
3-(nonylsulfonamidomethyl)benzoic acid
-
-
3-(octylsulfonamido)phenylphosphonic acid
-
-
3-(pentylsulfonamido)benzylphosphonic acid
-
-
3-(pentylsulfonamidomethyl)benzoic acid
-
-
3-(phenylmethylsulfonamido)benzoic acid
-
-
3-(phenylsulfonamido)benzoic acid
-
-
4-(2-phenylethylsulfonamido)benzoic acid
-
-
4-(4-chlorophenylsulfonamido)benzoic acid
-
-
4-(nonylsulfonamido)benzoic acid
-
-
4-(nonylsulfonamido)benzylphosphonic acid
-
-
4-(nonylsulfonamidomethyl)benzoic acid
-
-
4-(octylsulfonamido)phenylphosphonic acid
-
-
4-(pentylsulfonamido)benzylphosphonic acid
-
-
4-(pentylsulfonamidomethyl)benzoic acid
-
-
4-(phenylmethylsulfonamido)benzoic acid
-
-
4-(phenylsulfonamido)benzoic acid
-
-
5-chloro-2-(nonylsulfonamido)benzoic acid
-
-
5-fluoro-2-(octylsulfonamido)benzoic acid
-
-
5-hydroxy-2-(octylsulfonamido)benzoic acid
-
-
N-ethylmaleimide
additional information
-
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.295
(+-)-2-(2-hydroxy-3-(octylsulfonamido)-cyclopentyl)acetic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.164
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclohexanecarboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.255
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclopentane carboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.324
(+-)-2-hydroxy-3-(octylsulfonamido)-cyclopentanecarboxylic acid
Homo sapiens
-
pH not specified in the publication, temperature not specified in the publication
0.152
2-(2-phenylethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.107
2-(4-chlorophenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0183
2-(hexadecylsulfonamido)benzoic acid
Homo sapiens
-
-
0.132
2-(methylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0247
2-(nonylsulfonamido)benzoic acid
0.0811
2-(nonylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.0736
2-(octylsulfonamido)phenylphosphonic acid
Homo sapiens
-
-
0.129
2-(pentylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.14
2-(phenylmethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.146
2-(phenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0174
2-(tetradecylsulfonamido)benzoic acid
Homo sapiens
-
-
0.164
3-(2-phenylethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0757
3-(4-chlorophenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0739
3-(nonylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0628
3-(nonylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.0835
3-(nonylsulfonamidomethyl)benzoic acid
Homo sapiens
-
-
0.0724
3-(octylsulfonamido)phenylphosphonic acid
Homo sapiens
-
-
0.141
3-(pentylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.31
3-(pentylsulfonamidomethyl)benzoic acid
Homo sapiens
-
-
0.178
3-(phenylmethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.138
3-(phenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.206
4-(2-phenylethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.108
4-(4-chlorophenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0889
4-(nonylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0813
4-(nonylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.0665 - 0.129
4-(nonylsulfonamidomethyl)benzoic acid
0.0953
4-(octylsulfonamido)phenylphosphonic acid
Homo sapiens
-
-
0.148
4-(pentylsulfonamido)benzylphosphonic acid
Homo sapiens
-
-
0.217 - 0.252
4-(pentylsulfonamidomethyl)benzoic acid
0.221
4-(phenylmethylsulfonamido)benzoic acid
Homo sapiens
-
-
0.151
4-(phenylsulfonamido)benzoic acid
Homo sapiens
-
-
0.0318
5-chloro-2-(nonylsulfonamido)benzoic acid
Homo sapiens
-
-
0.089
5-fluoro-2-(octylsulfonamido)benzoic acid
Homo sapiens
-
-
0.116
5-hydroxy-2-(octylsulfonamido)benzoic acid
Homo sapiens
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
-
activity assay
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
activity assay at room temperature
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
isozyme GPAT2 is highly expressed in several cancer types (such as lung, melanoma, breast, and prostate cancer) and cancer-derived human cell lines
Manually annotated by BRENDA team
GPAT2 is mainly expressed in pachytene spermatocytes of testis
Manually annotated by BRENDA team
moderate expression
Manually annotated by BRENDA team
during lactation, GPAT4 mRNA is highly expressed in mammary gland epithelium, but not in the surrounding adipocytes of breast tissue
Manually annotated by BRENDA team
low expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
metabolism
physiological function
malfunction
metabolism
physiological function
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GPAT1_HUMAN
828
0
93795
Swiss-Prot
other Location (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48000
-
determined by SDS-PAGE and Western Blot analysis
50000
52000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by anti-FLAG M2 affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
GPAT3 is localized to the endoplasmic reticulum when overexpressed in COS-7 cells
-
into the mammalian expression vector p3xFLAG-CMV-14 for transfection of HEK-293 cells
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
carbohydrate response element-binding protein (ChREBP) and sterol regulatory element-binding protein-1c (SREBP-1c) are involved in the transcriptional upregulation of GPAT1 levels under increased dietary intake of carbohydrate and increased levels of insulin in plasma
the expression of mitochondrial GPAT, both at the mRNA and protein levels, is downregulated when targeted by upregulated miRNAs, such as miR-23b, in Epstein-Barr virus-infected B-cells under several culture conditions
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
GPAT3 may be useful as a target for the treatment of obesity
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cao, J.; Li, J.L.; Li, D.; Tobin, J.F.; Gimeno, R.E.
Molecular identification of microsomal acyl-CoA:glycerol-3-phosphate acyltransferase, a key enzyme in de novo triacylglycerol synthesis
Proc. Natl. Acad. Sci. USA
103
19695-19700
2006
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Chen, Y.Q.; Kuo, M.S.; Li, S.; Bui, H.H.; Peake, D.A.; Sanders, P.E.; Thibodeaux, S.J.; Chu, S.; Qian, Y.W.; Zhao, Y.; Bredt, D.S.; Moller, D.E.; Konrad, R.J.; Beigneux, A.P.; Young, S.G.; Cao, G.
AGPAT6 is a novel microsomal glycerol-3-phosphate acyltransferase
J. Biol. Chem.
283
10048-10057
2008
Homo sapiens
Manually annotated by BRENDA team
Takeuchi, K.; Reue, K.
Biochemistry, physiology, and genetics of GPAT, AGPAT, and lipin enzymes in triglyceride synthesis
Am. J. Physiol. Endocrinol. Metab.
296
E1195-E1209
2009
Rattus norvegicus, Rattus norvegicus (P97564), Mus musculus (Q14DK4), Mus musculus (Q61586), Mus musculus (Q8C0N2), Mus musculus (Q8K2C8), Mus musculus, Homo sapiens (Q53EU6), Homo sapiens (Q86UL3), Homo sapiens
Manually annotated by BRENDA team
Wydysh, E.A.; Medghalchi, S.M.; Vadlamudi, A.; Townsend, C.A.
Design and synthesis of small molecule glycerol 3-phosphate acyltransferase inhibitors
J. Med. Chem.
52
3317-3327
2009
Homo sapiens
Manually annotated by BRENDA team
Wydysh, E.A.; Vadlamudi, A.; Medghalchi, S.M.; Townsend, C.A.
Design, synthesis, and biological evaluation of conformationally constrained glycerol 3-phosphate acyltransferase inhibitors
Bioorg. Med. Chem.
18
6470-6479
2010
Homo sapiens, Cucurbita moschata (P10349)
Manually annotated by BRENDA team
Faris, R.; Weber, M.M.; Seeger, D.R.; Cavazos, D.; de Graffenried, L.; Murphy, E.J.; Jolly, C.A.
Mitochondrial glycerol-3-phosphate acyltransferase-dependent phospholipid synthesis modulates phospholipid mass and IL-2 production in Jurkat T cells
Lipids
51
291-301
2016
Homo sapiens
Manually annotated by BRENDA team
Lee, J.; Ridgway, N.
Substrate channeling in the glycerol-3-phosphate pathway regulates the synthesis, storage and secretion of glycerolipids
Biochim. Biophys. Acta
1865
158438
2020
Mus musculus (Q14DK4), Mus musculus (Q61586), Mus musculus (Q8C0N2), Mus musculus (Q8K2C8), Homo sapiens (Q53EU6), Homo sapiens (Q6NUI2), Homo sapiens (Q86UL3), Homo sapiens (Q9HCL2)
Manually annotated by BRENDA team
Yu, J.; Loh, K.; Song, Z.Y.; Yang, H.Q.; Zhang, Y.; Lin, S.
Update on glycerol-3-phosphate acyltransferases the roles in the development of insulin resistance
Nutr. Diabetes
8
34
2018
Mus musculus (Q14DK4), Mus musculus (Q61586), Mus musculus (Q8C0N2), Mus musculus (Q8K2C8), Homo sapiens (Q53EU6), Homo sapiens (Q86UL3), Homo sapiens (Q9HCL2), Mus musculus C57BL/6J (Q8K2C8)
Manually annotated by BRENDA team