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Information on EC 2.3.1.15 - glycerol-3-phosphate 1-O-acyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession P36148

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EC Tree
IUBMB Comments
Acyl-[acyl-carrier protein] can also act as acyl donor. The enzyme acts only on derivatives of fatty acids of chain length larger than C10.
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This record set is specific for:
Saccharomyces cerevisiae
UNIPROT: P36148
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The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
glycerol-3-phosphate acyltransferase, gpat1, glycerophosphate acyltransferase, sn-glycerol-3-phosphate acyltransferase, gpat2, gpat3, gpat4, agpat6, agpat, gat1p, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-glycerophosphate acyltransferase
-
-
-
-
ACP:sn-glycerol-3-phosphate acyltransferase
-
-
-
-
acyltransferase, glycerol phosphate
-
-
-
-
alpha-glycerophosphate acyltransferase
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-
-
-
glycerol 3-phosphate acyltransferase
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-
-
-
glycerol phosphate acyltransferase
-
-
-
-
glycerol phosphate transacylase
-
-
-
-
glycerol-3-phosphate acyltransferase 1
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-
glycerophosphate acyltransferase
-
-
-
-
glycerophosphate transacylase
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-
-
-
Sn-glycerol 3-phosphate acyltransferase
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-
-
-
additional information
-
enzyme may be identical with EC 2.3.1.42
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:sn-glycerol-3-phosphate 1-O-acyltransferase
Acyl-[acyl-carrier protein] can also act as acyl donor. The enzyme acts only on derivatives of fatty acids of chain length larger than C10.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-96-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
enzyme has both 3-glycerophosphate acyltransferase and dihydroxyacetone phosphate acyltransferase activity
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
palmitoleoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
stearoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
acyl-CoA + sn-glycerol 3-phosphate
CoA + 1-acyl-sn-glycerol 3-phosphate
show the reaction diagram
myristoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-myristoyl-sn-glycerol 3-phosphate
show the reaction diagram
n-dodecanoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-n-dodecanoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
i.e. lauroyl-CoA, poor substrate
-
?
oleoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-oleoyl-sn-glycerol 3-phosphate
show the reaction diagram
palmitoleoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-palmitoleoyl-sn-glycerol 3-phosphate
show the reaction diagram
-
-
?
palmitoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-palmitoyl-sn-glycerol 3-phosphate
show the reaction diagram
stearoyl-CoA + sn-glycerol 3-phosphate
CoA + 1-stearoyl-sn-glycerol 3-phosphate
show the reaction diagram
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
deoxycholate
-
above 0.35 mg/ml
dihydroxyacetone phosphate
-
-
N-ethylmaleimide
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0.4 mM, 60% inhibition after 10 min
palmitoyl-CoA
-
0.08 mM
Triton X-100
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strong, above 0.15 mg/ml
Trypsin
-
0.06 mg/ml, 80% inhibition after 10 min
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oleate
presence of oleate induces dephosphorylation of isoform Gat1p as well as an increment in its levels. In presence of oleate, Gat1p crescent structures are formed which are connected with the endoplasmic reticulum and are intimately associated with lipid particles
Triton X-100
-
i.e. octylphenoxy polyethoxyethanol, slight stimulation at 0.1 mg/ml
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
sn-glycerol 3-phosphate
-
-
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.54
dihydroxyacetone phosphate
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
50% of maximal activity at pH 7.4
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.6 - 8.9
-
maximal activity at pH 6.6, approx. 30% of maximal activity at pH 8.9
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
yeast strains lacking isoform Gat1p are sensitive to oleate and fail to accumulate lipid particles induced by this unsaturated fatty acid
malfunction
-
loss of function of Gat1p and Gat2p is masked by the compensatory effect of their redundant partner. The complete lack of GPAT results in cell death, with multibudded cells containing divided nuclei
physiological function
-
overexpression Gat1p in the absence of endogenous GPATs results in elongated cells with a normal cortical edoplasmic reticulum positioned underneath the plasma membrane, while excess Gat2p results in larger and round cells with an irregular layout of membranes, with pronounced invaginations also affecting the plasma membrane morphology
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phosphoprotein
presence of oleate induces dephosphorylation of isoform Gat1p
phosphoprotein
-
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, 3-5 mg/ml protein, membrane preparation, at least 2 months
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
expression in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
presence of oleate induces dephosphorylation of isoform Gat1p as well as an increment in its levels
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Schlossman, D.M.; Bell, R.M.
Glycerolipid biosynthesis in Saccharomyces cerevisiae: sn-glycerol-3-phosphate and dihydroxyacetone phosphate acyltransferase activities
J. Bacteriol.
133
1368-1376
1978
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Yamada, K.; Okuyama, H.; Endo, Y.; Ikezawa, H.
Acyltransferase systems involved in phospholipid metabolism in Saccharomyces cerevisiae
Arch. Biochem. Biophys.
183
281-289
1977
Saccharomyces cerevisiae, Saccharomyces cerevisiae OC-2
Manually annotated by BRENDA team
Zheng, Z.; Zou, J.
The initial step of the glycerolipid pathway: identification of glycerol 3-phosphate/dihydroxyacetone phosphate dual substrate acyltransferases in Saccharomyces cerevisiae
J. Biol. Chem.
276
41710-41716
2001
Saccharomyces cerevisiae (P32784), Saccharomyces cerevisiae (P36148), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Bratschi, M.W.; Burrowes, D.P.; Kulaga, A.; Cheung, J.F.; Alvarez, A.L.; Kearley, J.; Zaremberg, V.
Glycerol-3-phosphate acyltransferases gat1p and gat2p are microsomal phosphoproteins with differential contributions to polarized cell growth
Eukaryot. Cell
8
1184-1196
2009
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Marr, N.; Foglia, J.; Terebiznik, M.; Athenstaedt, K.; Zaremberg, V.
Controlling lipid fluxes at glycerol-3-phosphate acyltransferase step in yeast: unique contribution of Gat1p to oleic acid-induced lipid particle formation
J. Biol. Chem.
287
10251-10264
2012
Saccharomyces cerevisiae (P36148), Saccharomyces cerevisiae
Manually annotated by BRENDA team