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2,3-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-(2,3-dimethoxycinnamoyl)-3-hydroxyanthranilate
-
-
-
?
2,3-dimethoxycinnamoyl-CoA + anthranilate
CoA + N-(2,3-dimethoxycinnamoyl)-anthranilate
-
-
-
?
2,4-dimethoxycinnamoyl-CoA + anthranilate
CoA + N-(2,4-dimethoxycinnamoyl)-anthranilate
-
-
-
?
2,5-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-(2,5-dimethoxycinnamoyl)-3-hydroxyanthranilate
-
-
-
?
2,5-dimethoxycinnamoyl-CoA + anthranilate
CoA + N-(2,5-dimethoxycinnamoyl)-anthranilate
-
-
-
?
2-coumaroyl-CoA + 3-hydroxyanthranilate
CoA + N-(2-coumaroyl)-3-hydroxyanthranilate
-
-
-
?
2-coumaroyl-CoA + anthranilate
CoA + N-(2-coumaroyl)anthranilate
-
-
-
?
3,4,5-trimethoxycinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-(3,4,5-trimethoxycinnamoyl)-3-hydroxyanthranilate
-
-
-
?
3,4,5-trimethoxycinnamoyl-CoA + anthranilate
CoA + N-(3,4,5-trimethoxycinnamoyl)-anthranilate
-
-
-
?
3,4-dimethoxycinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-(3,4-dimethoxycinnamoyl)-3-hydroxyanthranilate
-
-
-
?
3-coumaroyl-CoA + 3-hydroxyanthranilate
CoA + N-(3-coumaroyl)-3-hydroxyanthranilate
-
-
-
?
3-coumaroyl-CoA + anthranilate
CoA + N-(3-hydroxycinnamoyl)-anthranilate
-
-
-
?
3-hydroxy-4-methoxycinnamoyl-CoA + anthranilate
CoA + N-(3-hydroxy-4-methoxycinnamoyl)-anthranilate
-
-
-
?
3-methoxycinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-(3-methoxycinnamoyl)-3-hydroxyanthranilate
-
-
-
?
3-methoxycinnamoyl-CoA + anthranilate
CoA + N-(3-methoxycinnamoyl)-anthranilate
-
-
-
?
4-coumaroyl-CoA + 3-hydroxyanthranilate
CoA + N-(4-coumaroyl)-3-hydroxyanthranilate
-
-
-
?
4-coumaroyl-CoA + anthranilate
CoA + N-(4-coumaroyl)-anthranilate
-
product detection by mass spectrometry
-
?
4-coumaroyl-CoA + anthranilate
CoA + N-4-(coumaroyl)anthranilate
4-methoxycinnamoyl-CoA + anthranilate
CoA + N-(4-methoxycinnamoyl)-anthranilate
-
-
-
?
benzoyl-CoA + 3-hydroxyanthranilate
CoA + N-benzoyl-3-hydroxyanthranilate
-
20% of activity with anthranilate
-
?
benzoyl-CoA + 4-hydroxyanthranilate
CoA + N-benzoyl-4-hydroxyanthranilate
-
10% of activity with anthranilate
-
?
benzoyl-CoA + anthranilate
CoA + N-benzoylanthranilate
caffeoyl-CoA + 3-hydroxyanthranilate
CoA + N-caffeoyl-3-hydroxyanthranilate
-
-
-
?
caffeoyl-CoA + anthranilate
CoA + N-(3,4-dihydroxycinnamoyl)-anthranilate
-
-
-
?
cinnamoyl-CoA + 3-hydroxyanthranilate
CoA + N-cinnamoyl-3-hydroxyanthranilate
-
-
-
?
cinnamoyl-CoA + anthranilate
CoA + N-cinammoylanthranilate
-
140% of activity with benzoyl-CoA
-
-
?
cinnamoyl-CoA + anthranilate
CoA + N-cinnamoylanthranilate
-
-
-
?
coumaroyl-CoA + anthranilate
CoA + N-(4-hydroxycinnamoyl)-anthranilate
-
product detection by mass spectrometry
-
?
feruloyl-CoA + 3-hydroxyanthranilate
CoA + N-feruloyl-3-hydroxyanthranilate
-
-
-
?
feruloyl-CoA + anthranilate
CoA + N-feruloylanthranilate
-
-
-
?
salicyl-CoA + anthranilate
CoA + N-salicylanthranilate
-
60% of activity with benzoyl-CoA
-
-
?
sinapoyl-CoA + 3-hydroxyanthranilate
CoA + N-sinapoyl-3-hydroxyanthranilate
-
-
-
?
sinapoyl-CoA + anthranilate
CoA + N-sinapoylanthranilate
-
-
-
?
additional information
?
-
4-coumaroyl-CoA + anthranilate
CoA + N-4-(coumaroyl)anthranilate
-
-
-
?
4-coumaroyl-CoA + anthranilate
CoA + N-4-(coumaroyl)anthranilate
-
127% of activity with benzoyl-CoA
-
-
?
benzoyl-CoA + anthranilate
CoA + N-benzoylanthranilate
-
-
-
?
benzoyl-CoA + anthranilate
CoA + N-benzoylanthranilate
-
cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors
-
-
benzoyl-CoA + anthranilate
CoA + N-benzoylanthranilate
-
cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors
-
?
benzoyl-CoA + anthranilate
CoA + N-benzoylanthranilate
-
cinnamoyl-CoA, salicyl-CoA and 4-coumaroyl-CoA can act as donors with 73%, 70% and 65% efficiency respectively, enzyme is involved in the biosynthesis of phytoalexins, 4-hydroxyanthranilate is the most probable substrate in vivo
-
?
additional information
?
-
-
hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase, HCBT, has affinity for anthranilate and 4-coumaroyl-CoA and is capable of producing N-(4-hydroxycinnamoyl)-anthranilate in vitro
-
-
-
additional information
?
-
hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase, HCBT, has affinity for anthranilate and 4-coumaroyl-CoA and is capable of producing N-(4-hydroxycinnamoyl)-anthranilate in vitro
-
-
-
additional information
?
-
-
by exploiting the substrate flexibility of both 4-coumaroyl:CoA ligase 4CL5 from Arabidopsis thaliana and HCBT, rapid biosynthesis of more than 160 cinnamoyl, dihydrocinnamoyl, and benzoyl anthranilates in yeast can be achieved. The enzyme offers flexibility for a wide range of acyl-CoA donors, its affinity towards acceptors seems limited to anthranilates
-
-
-
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Reinhard, K.; Matern, U.
The biosynthesis of phytoalexins in Dianthus caryophyllus L. cell cultures: induction of benzoyl-CoA:anthranilate N-benzoyltransferase activity
Arch. Biochem. Biophys.
275
295-301
1989
Dianthus caryophyllus
brenda
Yang, Q.; Reinhard, K.; Schiltz, E.; Matern, U.
Characterization and heterologous expression of hydroxycinnamoyl/benzoyl-CoA:anthranilate N-hydroxycinnamoyl/benzoyltransferase from elicited cell cultures of carnation, Dianthus caryophyllus L
Plant Mol. Biol.
35
777-789
1997
Dianthus caryophyllus
brenda
Yang, Q.; Grimmig, B.; Matern, U.
Anthranilate N-hydroxycinnamoyl/benzoyltransferase gene from carnation: rapid elicitation of transcription and promoter analysis
Plant Mol. Biol.
38
1201-1214
1998
Dianthus caryophyllus
brenda
Eudes, A.; Baidoo, E.E.; Yang, F.; Burd, H.; Hadi, M.Z.; Collins, F.W.; Keasling, J.D.; Loque, D.
Production of tranilast [N-(3,4-dimethoxycinnamoyl)-anthranilic acid] and its analogs in yeast Saccharomyces cerevisiae
Appl. Microbiol. Biotechnol.
89
989-1000
2011
Dianthus caryophyllus, Dianthus caryophyllus (O24645)
brenda
Eudes, A.; Juminaga, D.; Baidoo, E.E.; Collins, F.W.; Keasling, J.D.; Loque, D.
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli
Microb. Cell Fact.
12
62
2013
Dianthus caryophyllus (O24645)
brenda
Eudes, A.; Teixeira Benites, V.; Wang, G.; Baidoo, E.E.; Lee, T.S.; Keasling, J.D.; Loque, D.
Precursor-directed combinatorial biosynthesis of cinnamoyl, dihydrocinnamoyl, and benzoyl anthranilates in Saccharomyces cerevisiae
PLoS ONE
10
e0138972
2015
Dianthus caryophyllus (O24645)
brenda
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2.3.1.144 anthranilate N-benzoyltransferase
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