HOME
login
history
all enzymes
BRENDA home
History
Information on EC 2.3.1.138 - putrescine N-hydroxycinnamoyltransferase
for references in articles please use BRENDA:EC2.3.1.138Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
2.3.1.138 putrescine N-hydroxycinnamoyltransferaseIUBMB Comments
Feruloyl-CoA, cinnamoyl-CoA and sinapoyl-CoA can also act as donors, but more slowly.
Specify your search results
The enzyme appears in viruses and cellular organisms
Synonyms
AT1, caffeoyl-CoA putrescine N-caffeoyl transferase, hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase, hydroxycinnamoyl-coenzyme A:putrescine acyltransferase, Os09g0544000, PHT, putrescine hydroxycinnamoyl transferase, putrescine hydroxycinnamoyltransferase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
caffeoyl-CoA + putrescine = CoA + N-caffeoylputrescine
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
caffeoyl-CoA:putrescine N-(3,4-dihydroxycinnamoyl)transferase
Feruloyl-CoA, cinnamoyl-CoA and sinapoyl-CoA can also act as donors, but more slowly.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
120598-69-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-coumaroyl-CoA + putrescine
CoA + N-coumaroylputrescine
-
-
-
?
4-fluorocinnamoyl-CoA + putrescine
CoA + N-4-fluorocinnamoylputrescine
-
-
-
-
?
caffeoyl-CoA + diaminoheptane
CoA + N-caffeoyl-1,7-diaminoheptane
-
low activity
-
-
?
caffeoyl-CoA + diaminohexane
CoA + N-caffeoyl-1,6-diaminohexane
-
low activity
-
-
?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
-
-
-
-
?
caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
-
cadaverine i.e. 1,5-pentanediamine, low activity
-
-
?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
-
-
-
-
?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
-
-
-
-
?
caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
-
-
-
-
?
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
the enzyme is highly specific for putrescine and caffeoyl-CoA
-
-
?
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
-
-
-
-
?
cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
-
-
-
-
?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
feruloyl-CoA gives 10% of the activity with caffeoyl-CoA
-
-
?
feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
-
-
-
-
?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
-
-
-
-
?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
-
low activity
-
-
?
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
-
low activity
-
-
?
additional information
?
-
substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
-
-
?
additional information
?
-
-
substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
-
-
?
additional information
?
-
very poor substrate: caffeoyl-CoA
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.024
caffeoyl-CoA
with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C
0.1851
putrescine
cosubstrate 4-coumaroyl-CoA, pH 7.5, 30°C
0.664
putrescine
with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7.8 - 10
-
at 7.8 about 50% of activity maximum, at 10.0 activity maximum, caffeoyl-CoA + putrescine
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
barley, after 1-4d of infection with Blumeria graminis f. sp. hordei, increased activity
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
deregulatory effects of transcription factor MYB8 silencing on AT1 control, altered metabolite profile, overview. Silencing of acyltransferase AT1 impairs polyamine accumulation
metabolism
the enzyme AT1 is involved in a branch of jasmonic acid signaling that defends Nicotiana attenuata plants against herbivores: jasmonic acid, via key transcription factor MYB8's transcriptional control of AT1 and DH29 genes, controls the entire branch of polyamine biosynthesis, overview. AT1, DH29, and CV86 contribute to distinct, but interconnected, MYB8-controlled hydroxycinnamoylputrescine and hydroxycinnamoylspermidine pools
physiological function
the enzyme is responsible for caffeoylputrescine biosynthesis in tobacco
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
K267E
-
missense mutation identified in cv. Shepody, predicted to change the catalytic activity
P101T/S368N
-
missense mutation identified in cv. Shepody, predicted not to change the catalytic activity
T115M
-
missense mutation identified in cv. Shepody, predicted to change the catalytic activity
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His6-tagged AT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AT1 gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged AT1 in Escherichia coli strain BL21(DE3)
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression in leaf is highly induced upon herbivore feeding
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
-
transcript abundances of genes that catalyze the biosynthesis of hydroxycinnamic acid amides, such as 4-coumarate:CoA ligase, tyrosine decarboxylase, ornithine decarboxylase, tyramine hydroxycinnamoyl transferase, and putrescine hydroxycinnamoyl transferase, are higher in the genotypes resistant to late blight induced by Phytophthora infestans
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cowley, T.; Walters, D.R.
Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f. sp. hordei
Plant Cell Environ.
25
461-468
2002
Hordeum vulgare
-
brenda
Negrel, J.; Javelle, F.; Paynot, M.
Separation of putrescine and spermidine hydroxycinnamoyl transferases extracted from tobacco callus
Phytochemistry
30
1089-1092
1991
Nicotiana tabacum
-
brenda
Negrel, J.; Paynot, M.; Javelle, F.
Purification and properties of putrescine hydroxycinnamoyl transferase from tobacco (Nicotiana tabaccum) cell suspensions
Plant Physiol.
98
1264-1269
1992
Nicotiana tabacum, Nicotiana tabacum TX1
brenda
Meurer-Grimes, B.; Berlin, J.; Strack, D.
Hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase in tobacco cell cultures with high and low levels of caffeoylputrescine
Plant Physiol.
89
488-492
1989
Nicotiana tabacum, Nicotiana tabacum TX1, Nicotiana tabacum TX4
brenda
Negrel, J.
The biosynthesis of cinnamoylputrescines in callus tissue cultures of Nicotiana tabaccum
Phytochemistry
28
477-481
1989
Nicotiana tabacum
-
brenda
Onkokesung, N.; Gaquerel, E.; Kotkar, H.; Kaur, H.; Baldwin, I.; Galis, I.
MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A: polyamine transferases in Nicotiana attenuata
Plant Physiol.
158
389-407
2012
Nicotiana attenuata (G9HTF7), Nicotiana attenuata
brenda
Pushpa, D.; Yogendra, K.; Gunnaiah, R.; Kushalappa, A.; Murphy, A.
Identification of late blight resistance-related metabolites and genes in potato through nontargeted metabolomics
Plant Mol. Biol. Rep.
32
584-595
2014
Solanum tuberosum
-
brenda
Tanabe, K.; Hojo, Y.; Shinya, T.; Galis, I.
Molecular evidence for biochemical diversification of phenolamide biosynthesis in rice plants
J. Integr. Plant Biol.
58
903-913
2016
Oryza sativa Japonica Group (Q7XXN4)
brenda
Select items on the left to see more content.
EXTERNAL LINKS (specific for EC-Number 2.3.1.138)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Information
