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Information on EC 2.3.1.138 - putrescine N-hydroxycinnamoyltransferase for references in articles please use BRENDA:EC2.3.1.138Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The enzyme appears in viruses and cellular organisms
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putrescine N-hydroxycinnamoyltransferase
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caffeoyl-CoA + putrescine = CoA + N-caffeoylputrescine
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Acyl group transfer
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caffeoyl-CoA:putrescine N-(3,4-dihydroxycinnamoyl)transferase
Feruloyl-CoA, cinnamoyl-CoA and sinapoyl-CoA can also act as donors, but more slowly.
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caffeoyl-CoA putrescine N-caffeoyl transferase
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hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase
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hydroxycinnamoyl-coenzyme A:putrescine acyltransferase
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putrescine hydroxycinnamoyl transferase
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putrescine hydroxycinnamoyltransferase
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barley, after 1-4d of infection with Blumeria graminis f. sp. hordei, increased activity
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brenda
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UniProt
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TX1
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TX4
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brenda
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brenda
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brenda
TX1
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brenda
TX1; TX4
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brenda
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malfunction
deregulatory effects of transcription factor MYB8 silencing on AT1 control, altered metabolite profile, overview. Silencing of acyltransferase AT1 impairs polyamine accumulation
metabolism
the enzyme AT1 is involved in a branch of jasmonic acid signaling that defends Nicotiana attenuata plants against herbivores: jasmonic acid, via key transcription factor MYB8's transcriptional control of AT1 and DH29 genes, controls the entire branch of polyamine biosynthesis, overview. AT1, DH29, and CV86 contribute to distinct, but interconnected, MYB8-controlled hydroxycinnamoylputrescine and hydroxycinnamoylspermidine pools
physiological function
the enzyme is responsible for caffeoylputrescine biosynthesis in tobacco
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4-fluorocinnamoyl-CoA + putrescine
CoA + N-4-fluorocinnamoylputrescine
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
caffeoyl-CoA + diaminoheptane
CoA + N-caffeoyl-1,7-diaminoheptane
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low activity
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caffeoyl-CoA + diaminohexane
CoA + N-caffeoyl-1,6-diaminohexane
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low activity
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caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
coumaroyl-CoA + putrescine
CoA + N-coumaroylputrescine
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feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
additional information
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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cadaverine i.e. 1,5-pentanediamine, low activity
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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caffeoyl-CoA + cadaverine
CoA + N-caffeoylcadaverine
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caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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caffeoyl-CoA + diaminopropane
CoA + N-caffeoyl-1,3-diaminopropane
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
the enzyme is highly specific for putrescine and caffeoyl-CoA
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
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cinnamoyl-CoA + putrescine
CoA + N-cinnamoylputrescine
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feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
feruloyl-CoA gives 10% of the activity with caffeoyl-CoA
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feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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feruloyl-CoA + putrescine
CoA + N-feruloylputrescine
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sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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sinapoyl-CoA + putrescine
CoA + N-sinapoylputrescine
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low activity
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additional information
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substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
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additional information
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substrate specificity of recombinant His-tagged AT1, overview. No activity with spermidine, spermine, or 4-coumaroyl-CoA
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additional information
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no activity with spermidine
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additional information
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no activity with monoamines
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additional information
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no activity with monoamines
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
G9HTF7
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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caffeoyl-CoA + putrescine
CoA + N-caffeoylputrescine
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additional information
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no inhibition by thiol reagents
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0.003 - 0.033
caffeoyl-CoA
0.0025
cinnamoyl-CoA
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with putrescine
0.0125
Feruloyl-CoA
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with caffeoyl-CoA
0.003
caffeoyl-CoA
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pH 10.0
0.024
caffeoyl-CoA
with putrescine, recombinant His-tagged enzyme, pH 7.5, 30°C
0.033
caffeoyl-CoA
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pH 7.8
0.01
putrescine
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pH 10.0
0.01
putrescine
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caffeoyl-CoA with 1 mM putrescine
0.05
putrescine
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with 0.25 mM caffeoyl-CoA
0.664
putrescine
with caffeoyl-CoA, recombinant His-tagged enzyme, pH 7.5, 30°C
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caffeoyl-CoA, sinapoyl-CoA
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7.2 - 10
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about 50% of activity maximum at pH 7.2 and 10
7.5 - 11
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about 50% of activity maximum at pH 7.5 and 11
7.8 - 10
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at 7.8 about 50% of activity maximum, at 10.0 activity maximum, caffeoyl-CoA + putrescine
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30
assay at
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cultured
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45000
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HPLC gel filtration
50000
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1 * 50000, SDS-PAGE
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monomer
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1 * 50000, SDS-PAGE
monomer
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1 * 50000, SDS-PAGE
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thawing and refreezing, 20% loss of activity
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rapid denaturation in absence of reducing compounds
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486315
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-20°C, stable for 2 months
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recombinant C-terminally His6-tagged AT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
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AT1 gene, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of C-terminally His6-tagged AT1 in Escherichia coli strain BL21(DE3)
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AT1 expression is controlled by the key transcription factor MYB8
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K267E
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missense mutation identified in cv. Shepody, predicted to change the catalytic activity
P101T/S368N
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missense mutation identified in cv. Shepody, predicted not to change the catalytic activity
T115M
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missense mutation identified in cv. Shepody, predicted to change the catalytic activity
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agriculture
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transcript abundances of genes that catalyze the biosynthesis of hydroxycinnamic acid amides, such as 4-coumarate:CoA ligase, tyrosine decarboxylase, ornithine decarboxylase, tyramine hydroxycinnamoyl transferase, and putrescine hydroxycinnamoyl transferase, are higher in the genotypes resistant to late blight induced by Phytophthora infestans
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G9HTF7_NICAT
438
49686
TrEMBL
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Cowley, T.; Walters, D.R.
Polyamine metabolism in barley reacting hypersensitively to the powdery mildew fungus Blumeria graminis f. sp. hordei
Plant Cell Environ.
25
461-468
2002
Hordeum vulgare
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brenda
Negrel, J.; Javelle, F.; Paynot, M.
Separation of putrescine and spermidine hydroxycinnamoyl transferases extracted from tobacco callus
Phytochemistry
30
1089-1092
1991
Nicotiana tabacum
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brenda
Negrel, J.; Paynot, M.; Javelle, F.
Purification and properties of putrescine hydroxycinnamoyl transferase from tobacco (Nicotiana tabaccum) cell suspensions
Plant Physiol.
98
1264-1269
1992
Nicotiana tabacum, Nicotiana tabacum TX1
brenda
Meurer-Grimes, B.; Berlin, J.; Strack, D.
Hydroxycinnamoyl-CoA:putrescine hydroxycinnamoyltransferase in tobacco cell cultures with high and low levels of caffeoylputrescine
Plant Physiol.
89
488-492
1989
Nicotiana tabacum, Nicotiana tabacum TX1, Nicotiana tabacum TX4
brenda
Negrel, J.
The biosynthesis of cinnamoylputrescines in callus tissue cultures of Nicotiana tabaccum
Phytochemistry
28
477-481
1989
Nicotiana tabacum
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brenda
Onkokesung, N.; Gaquerel, E.; Kotkar, H.; Kaur, H.; Baldwin, I.; Galis, I.
MYB8 controls inducible phenolamide levels by activating three novel hydroxycinnamoyl-coenzyme A: polyamine transferases in Nicotiana attenuata
Plant Physiol.
158
389-407
2012
Nicotiana attenuata, Nicotiana attenuata (G9HTF7)
brenda
Pushpa, D.; Yogendra, K.; Gunnaiah, R.; Kushalappa, A.; Murphy, A.
Identification of late blight resistance-related metabolites and genes in potato through nontargeted metabolomics
Plant Mol. Biol. Rep.
32
584-595
2014
Solanum tuberosum
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brenda
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