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Information on EC 2.3.1.132 - glucarolactone O-hydroxycinnamoyltransferase for references in articles please use BRENDA:EC2.3.1.132
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EC Tree
IUBMB Comments 4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
The expected taxonomic range for this enzyme is: Secale cereale
Synonyms
hydroxycinnamic acid transferase,
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hydroxycinnamic acid transferase
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sinapoyl-CoA + glucarolactone = CoA + O-sinapoylglucarolactone
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Acyl group transfer
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sinapoyl-CoA:glucarolactone O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
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caffeoyl-CoA + glucarolactone
CoA + O-caffeoylglucarolactone
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poor substrate
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feruloyl-CoA + glucarolactone
CoA + O-feruloylglucarolactone
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condensation at 37% the rate of the reaction with sinapoyl-CoA
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p-coumaroyl-CoA + glucarolactone
CoA + O-p-coumaroylglucarolactone
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condensation at 54% the rate of the reaction with sinapoyl-CoA
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sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
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best substrate
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additional information
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no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
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sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
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best substrate
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7.8
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50 mM potassium phosphate buffer
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7.4 - 8.2
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about 80% of maximal activity at pH 7.4 and pH 8.2
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rye, var. Kustro
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brenda
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brenda
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80
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denaturation after 5 min
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-20°C, at least 6 months
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Strack, D.; Keller, H.; Weissenböck, G.
Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves
J. Plant Physiol.
131
61-73
1987
Secale cereale
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brenda
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