Information on EC 2.3.1.132 - glucarolactone O-hydroxycinnamoyltransferase

for references in articles please use BRENDA:EC2.3.1.132
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The expected taxonomic range for this enzyme is: Secale cereale

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EC NUMBER
COMMENTARY hide
2.3.1.132
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RECOMMENDED NAME
GeneOntology No.
glucarolactone O-hydroxycinnamoyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sinapoyl-CoA + glucarolactone = CoA + O-sinapoylglucarolactone
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
hydroxycinnamate sugar acid ester biosynthesis
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-
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SYSTEMATIC NAME
IUBMB Comments
sinapoyl-CoA:glucarolactone O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY hide
112956-52-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye, var. Kustro
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + glucarolactone
CoA + O-caffeoylglucarolactone
show the reaction diagram
-
poor substrate
-
?
feruloyl-CoA + glucarolactone
CoA + O-feruloylglucarolactone
show the reaction diagram
-
condensation at 37% the rate of the reaction with sinapoyl-CoA
-
?
p-coumaroyl-CoA + glucarolactone
CoA + O-p-coumaroylglucarolactone
show the reaction diagram
-
condensation at 54% the rate of the reaction with sinapoyl-CoA
-
?
sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
show the reaction diagram
-
best substrate
-
?
additional information
?
-
-
no activity with 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
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-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
sinapoyl-CoA + glucarolactone
CoA + O-sinapoylglucarolactone
show the reaction diagram
-
best substrate
-
?
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
DTT
-
activates
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10
glucarolactone
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-
0.029
Sinapoyl-CoA
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-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.8
-
50 mM potassium phosphate buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4 - 8.2
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about 80% of maximal activity at pH 7.4 and pH 8.2
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
-
denaturation after 5 min
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.132)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)