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Information on EC 2.3.1.131 - glucarate O-hydroxycinnamoyltransferase for references in articles please use BRENDA:EC2.3.1.131Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
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The expected taxonomic range for this enzyme is: Secale cereale
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glucarate O-hydroxycinnamoyltransferase
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sinapoyl-CoA + glucarate = CoA + O-sinapoylglucarate
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Acyl group transfer
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hydroxycinnamate sugar acid ester biosynthesis
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sinapoyl-CoA:glucarate O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
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hydroxycinnamoyltransferase, glucarate
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rye, var. Kustro
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brenda
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caffeoyl-CoA + glucarate
CoA + caffeoylglucarate
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condensation at 8% the rate of the reaction with sinapoyl-CoA
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feruloyl-CoA + glucarate
CoA + feruloylglucarate
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condensation at 54% the rate of the reaction with sinapoyl-CoA
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p-coumaroyl-CoA + glucarate
CoA + p-coumaroylglucarate
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condensation at 85% the rate of the reaction with sinapoyl-CoA
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sinapoyl-CoA + glucarate
CoA + O-sinapoylglucarate
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best substrate
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additional information
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no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
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0.01
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feruloyl-CoA as donor
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7.3
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100 mM potassium phosphate buffer
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6.2 - 8
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about 80% of maximal activity at pH 6.2 and 8.0
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primary
brenda
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80
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denaturation after 5 min
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-20°C, at least 6 months
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Strack, D.; Keller, H.; Weissenböck, G.
Enzymatic synthesis of hydroxycinnamic acid esters of sugar acids and hydroaromatic acids by protein preparations from rye (Secale cereale) primary leaves
J. Plant Physiol.
131
61-73
1987
Secale cereale
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brenda
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