Information on EC 2.3.1.131 - glucarate O-hydroxycinnamoyltransferase

for references in articles please use BRENDA:EC2.3.1.131
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The expected taxonomic range for this enzyme is: Secale cereale

top print hide
EC NUMBER
COMMENTARY hide
2.3.1.131
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top print hide
RECOMMENDED NAME
GeneOntology No.
glucarate O-hydroxycinnamoyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
sinapoyl-CoA + glucarate = CoA + O-sinapoylglucarate
show the reaction diagram
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-
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
hydroxycinnamate sugar acid ester biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
sinapoyl-CoA:glucarate O-(hydroxycinnamoyl)transferase
4-Coumaroyl-CoA, feruloyl-CoA and caffeoyl-CoA can also act as donors, but more slowly.
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CAS REGISTRY NUMBER
COMMENTARY hide
112956-51-1
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top print hide
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
rye, var. Kustro
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-
Manually annotated by BRENDA team
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
caffeoyl-CoA + glucarate
CoA + caffeoylglucarate
show the reaction diagram
-
condensation at 8% the rate of the reaction with sinapoyl-CoA
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?
feruloyl-CoA + glucarate
CoA + feruloylglucarate
show the reaction diagram
-
condensation at 54% the rate of the reaction with sinapoyl-CoA
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?
p-coumaroyl-CoA + glucarate
CoA + p-coumaroylglucarate
show the reaction diagram
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condensation at 85% the rate of the reaction with sinapoyl-CoA
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?
sinapoyl-CoA + glucarate
CoA + O-sinapoylglucarate
show the reaction diagram
-
best substrate
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?
additional information
?
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no substrates are 1-hydroxycinnamoyl glucose esters, gluconolactone, galactonate, glucuronate, ascorbate, malate, m-tartrate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
activation
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
8
glucarate
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0.018
Sinapoyl-CoA
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.01
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feruloyl-CoA as donor
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.3
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100 mM potassium phosphate buffer
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8
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about 80% of maximal activity at pH 6.2 and 8.0
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
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assay at
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
primary
Manually annotated by BRENDA team
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
80
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denaturation after 5 min
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, at least 6 months
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.131)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)