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Information on EC 2.3.1.13 - glycine N-acyltransferase and Organism(s) Mus musculus and UniProt Accession Q91XE0

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EC Tree
     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.13 glycine N-acyltransferase
IUBMB Comments
The CoA derivatives of a number of aliphatic and aromatic acids, but not phenylacetyl-CoA or (indol-3-yl)acetyl-CoA, can act as donor. Not identical with EC 2.3.1.68 glutamine N-acyltransferase or EC 2.3.1.71 glycine N-benzoyltransferase.
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This record set is specific for:
Mus musculus
UNIPROT: Q91XE0
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
Synonyms
glyat, glyatl1, glycine n-acyltransferase, glycine-n-acyltransferase, glycine-n-acylase, acyl-coa:glycine n-acyltransferase, glycine acyltransferase, glyatl3, glyatl2, glycine-n-acyltransferase like 1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acyltransferase, glycine
-
-
-
-
glycine acyltransferase
-
-
-
-
glycine N-acyltransferase-like 3
-
glycine-N-acylase
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
acyl-CoA:glycine N-acyltransferase
The CoA derivatives of a number of aliphatic and aromatic acids, but not phenylacetyl-CoA or (indol-3-yl)acetyl-CoA, can act as donor. Not identical with EC 2.3.1.68 glutamine N-acyltransferase or EC 2.3.1.71 glycine N-benzoyltransferase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl-CoA + glycine
CoA + N-acetylglycine
show the reaction diagram
-
-
-
?
benzoyl-CoA + glycine
CoA + N-benzoylglycine
show the reaction diagram
benzoyl-CoA + glycine ethyl ester
CoA + N-benzoylglycine ethyl ester
show the reaction diagram
-
-
-
?
benzoyl-CoA + glycine methyl ester
CoA + N-benzoylglycine methyl ester
show the reaction diagram
-
-
-
?
butyryl-CoA + glycine
CoA + N-butyrylglycine
show the reaction diagram
-
-
-
?
hexanoyl-CoA + glycine
CoA + N-hexanoylglycine
show the reaction diagram
-
-
-
?
acyl-CoA + glycine
CoA + N-acylglycine
show the reaction diagram
-
-
-
?
oleoyl-CoA + glycine
CoA + N-oleoylglycine
show the reaction diagram
-
-
-
?
palmitoyl-CoA + glycine
CoA + N-palmitoylglycine
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
acyl-CoA + glycine
CoA + N-acylglycine
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanolamine
about 37% inhibition at 100 mM
Gly-Gly-Gly
about 37% inhibition at 100 mM
glycolic acid
-
oleoyl-CoA
-
serine
about 15% inhibition at 100 mM
tyramine
about 53% inhibition at 100 mM
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.209
acetyl-CoA
0.0094
benzoyl-CoA
0.052
butyryl-CoA
6.1
glycine
apparent value, at pH 8.0, temperature not specified in the publication
9.7
glycine ethyl ester
apparent value, at pH 8.0, temperature not specified in the publication
29
Glycine methyl ester
apparent value, at pH 8.0, temperature not specified in the publication
0.045 - 450
hexanoyl-CoA
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.8
acetyl-CoA
4.2
benzoyl-CoA
15
butyryl-CoA
3.2
glycine
apparent value, at pH 8.0, temperature not specified in the publication
0.57
glycine ethyl ester
apparent value, at pH 8.0, temperature not specified in the publication
1
Glycine methyl ester
apparent value, at pH 8.0, temperature not specified in the publication
4.6
hexanoyl-CoA
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3.8
acetyl-CoA
450
benzoyl-CoA
290
butyryl-CoA
0.52
glycine
apparent value, at pH 8.0, temperature not specified in the publication
0.059
glycine ethyl ester
apparent value, at pH 8.0, temperature not specified in the publication
0.035
Glycine methyl ester
apparent value, at pH 8.0, temperature not specified in the publication
100
hexanoyl-CoA
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
58
glycolic acid
Mus musculus
apparent value, at pH 8.0, temperature not specified in the publication
0.021
oleoyl-CoA
Mus musculus
at pH 8.0, temperature not specified in the publication
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
siRNA knockdown of GLYATL3 in N18TG2 cells results in a decrease in the levels of N-acylglycines and primary fatty acid amides
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
GLYAT_MOUSE
296
0
34098
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
34000
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 33000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
nickel affinity column chromatography, and gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dempsey, D.R.; Bond, J.D.; Carpenter, A.M.; Rodriguez Ospina, S.; Merkler, D.J.
Expression, purification, and characterization of mouse glycine N-acyltransferase in Escherichia coli
Protein Expr. Purif.
97
23-28
2014
Mus musculus (Q91XE0), Mus musculus
Manually annotated by BRENDA team
Jeffries, K.A.; Dempsey, D.R.; Farrell, E.K.; Anderson, R.L.; Garbade, G.J.; Gurina, T.S.; Gruhonjic, I.; Gunderson, C.A.; Merkler, D.J.
Glycine N-acyltransferase-like 3 is responsible for long-chain N-acylglycine formation in N18TG2 cells
J. Lipid Res.
57
781-790
2016
Mus musculus, Mus musculus (E9Q5L8)
Manually annotated by BRENDA team