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Information on EC 2.3.1.101 - formylmethanofuran-tetrahydromethanopterin N-formyltransferase and Organism(s) Methanothermobacter thermautotrophicus and UniProt Accession P21348

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IUBMB Comments
Methanofuran is a complex 4-substituted furfurylamine and is involved in the formation of methane from CO2 in Methanobacterium thermoautotrophicum.
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This record set is specific for:
Methanothermobacter thermautotrophicus
UNIPROT: P21348
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Word Map
The taxonomic range for the selected organisms is: Methanothermobacter thermautotrophicus
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
formyltransferase, formylmethanofuran:tetrahydromethanopterin formyltransferase, formylmethanofuran tetrahydromethanopterin formyltransferase, formyltransferase/hydrolase complex, n-formylmethanofuran(cho-mfr):tetrahydromethanopterin(h4mpt) formyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
formylmethanofuran:5,6,7,8-tetrahydromethanopterin N5-formyltransferase
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formylmethanofuran:tetrahydromethanopterin formyltransferase
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formyltransferase, formylmethanofuran-tetrahydromethanopterin
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formyltransferase/hydrolase complex
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FTR
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N-formylmethanofuran(CHO-MFR):tetrahydromethanopterin(H4MPT) formyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin = methanofuran + 5-formyl-5,6,7,8-tetrahydromethanopterin
show the reaction diagram
ternary complex type mechanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
formylmethanofuran:5,6,7,8-tetrahydromethanopterin 5-formyltransferase
Methanofuran is a complex 4-substituted furfurylamine and is involved in the formation of methane from CO2 in Methanobacterium thermoautotrophicum.
CAS REGISTRY NUMBER
COMMENTARY hide
105669-83-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
show the reaction diagram
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-
?
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
show the reaction diagram
N-furfurylformamide + 5,6,7,8-tetrahydromethanopterin
2-(aminomethyl)furane + N5-formyl-5,6,7,8-tetrahydromethanopterin
show the reaction diagram
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pseudo-substrate
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-
?
additional information
?
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not: N-methylformamide, formamide, formate
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
formylmethanofuran + 5,6,7,8-tetrahydromethanopterin
methanofuran + N5-formyl-5,6,7,8-tetrahydromethanopterin
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.05 - 0.06
formylmethanofuran
70
N-Furfurylformamide
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2770
5,6,7,8-tetrahydromethanopterin
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
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1 * 32000 SDS-PAGE
41000
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4 * 41000, SDS-PAGE before and after treatment with dimethylsuberimidate
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
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1 * 32000 SDS-PAGE
tetramer
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4 * 41000, SDS-PAGE before and after treatment with dimethylsuberimidate
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Breitung, J.; Brner, G.; Karrasch, M.; Berkessel, A.; Thauer, R.K.
N-Furfurylformamide as a pseudo-substrate for formylmethanofuran converting enzymes from methanogenic bacteria
FEBS Lett.
268
257-260
1990
Methanosarcina barkeri, Methanosarcina barkeri Fusaro / DSM 804, Methanothermobacter thermautotrophicus
Manually annotated by BRENDA team
Donnelly, M.I.; Wolfe, R.S.
The role of formylmethanofuran:tetrahydromethanopterin formyltransferase in methanogenesis from carbon dioxide
J. Biol. Chem.
261
16652-16659
1986
Methanothermobacter thermautotrophicus
-
Manually annotated by BRENDA team
Leigh, J.A.; Rinehart, K.L.; Wolfe, R.S.
Structure of methanofuran, the carbon dioxide reduction factor of Methanobacterium thermoautotrophicum
J. Am. Chem. Soc.
106
3636-3640
1984
Methanothermobacter thermautotrophicus
-
Manually annotated by BRENDA team
DiMarco, A.A.; Sment, K.A.; Konisky, J.; Wolfe, R.S.
The formylmethanofuran: tetrahydromethanopterin formyltransferase from Methanobacterium thermoautotrophicum delta H. Nucleotide sequence and functional expression of the cloned gene
J. Biol. Chem.
265
472-476
1990
Methanothermobacter thermautotrophicus (P21348), Methanothermobacter thermautotrophicus
Manually annotated by BRENDA team
Breitung, J.; Thauer, R.K.
Formylmethanofuran: tetrahydromethanopterin formyltransferase from Methanosarcina barkeri. Identification of N5-formyltetrahydromethanopterin as the product
FEBS Lett.
275
226-230
1990
Methanosarcina barkeri, Methanothermobacter thermautotrophicus
Manually annotated by BRENDA team