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Information on EC 2.2.1.8 - fluorothreonine transaldolase and Organism(s) Streptomyces cattleya and UniProt Accession B6VP39

for references in articles please use BRENDA:EC2.2.1.8
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EC Tree
IUBMB Comments
A pyridoxal phosphate protein. Can also convert chloroacetaldehyde into4-chloro-L-threonine. Unlike EC 2.1.2.1, glycine hydroxymethyltransferase, does not use glycine as a substrate.
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This record set is specific for:
Streptomyces cattleya
UNIPROT: B6VP39
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The taxonomic range for the selected organisms is: Streptomyces cattleya
The enzyme appears in selected viruses and cellular organisms
Synonyms
4-fluorothreonine transaldolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4-fluorothreoinine transaldolase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
aldehyde group transfer
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
fluoroacetaldehyde:L-threonine aldehydetransferase
A pyridoxal phosphate protein. Can also convert chloroacetaldehyde into4-chloro-L-threonine. Unlike EC 2.1.2.1, glycine hydroxymethyltransferase, does not use glycine as a substrate.
CAS REGISTRY NUMBER
COMMENTARY hide
403503-13-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-threonine + fluoroacetaldehyde
4-fluoro-L-threonine + acetaldehyde
show the reaction diagram
-
-
-
?
fluoroacetaldehyde + L-threonine
acetaldehyde + 4-fluoro-L-threonine
show the reaction diagram
-
-
-
?
L-threonine + chloroacetaldehyde
acetaldehyde + 4-chloro-L-threonine
show the reaction diagram
-
-
-
?
L-threonine + fluoroacetaldehyde
acetaldehyde + 4-fluoro-L-threonine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-threonine + fluoroacetaldehyde
4-fluoro-L-threonine + acetaldehyde
show the reaction diagram
-
-
-
?
L-threonine + fluoroacetaldehyde
acetaldehyde + 4-fluoro-L-threonine
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
-
pyridoxal 5'-phosphate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
final step of 4-fluorothreonine biosynthesis
physiological function
gene disruption of the transaldolase results in a mutant which can produce fluoroacetate but is blocked in its ability to biosynthesise 4-fluorothreonine, revealing a branchpoint role for the PLP-transaldolase
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
4FTAS_STRCT
634
0
69529
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
120000
-
gel filtration
60000
-
2 * 60000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
2 * 60000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partially by Ni-affinity column, size exclusion chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21, transfection to Streptococcus lividans TK24
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Murphy, C.D.; Schaffrath, C.; O'Hagan, D.
Fluorinated natural products: the biosynthesis of fluoroacetate and 4-fluorothreonine in Streptomyces cattleya
Chemosphere
52
455-461
2003
Streptomyces cattleya, Streptomyces cattleya NRRL 8057
Manually annotated by BRENDA team
Murphy, C.D.; O'Hagan, D.; Schaffrath, C.
Identification of a PLP-dependent threonine transaldolase: a novel enzyme involved in 4-fluorothreonine biosynthesis in Streptomyces cattleya
Angew. Chem.
40
4479-4481
2001
Streptomyces cattleya, Streptomyces cattleya NRRL 8057
Manually annotated by BRENDA team
Deng, H.; Cross, S.M.; McGlinchey, R.P.; Hamilton, J.T.; OHagan, D.
In vitro reconstituted biotransformation of 4-fluorothreonine from fluoride ion: application of the fluorinase
Chem. Biol.
15
1268-1276
2008
Streptomyces cattleya (B6VP39)
Manually annotated by BRENDA team
Zhao, C.; Li, P.; Deng, Z.; Ou, H.Y.; McGlinchey, R.P.; OHagan, D.
Insights into fluorometabolite biosynthesis in Streptomyces cattleya DSM46488 through genome sequence and knockout mutants
Bioorg. Chem.
44
1-7
2012
Streptomyces cattleya (F8JMF0), Streptomyces cattleya DSM 46488 (F8JMF0)
Manually annotated by BRENDA team