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2,3-dichloro-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-(1,1-dihydroxyethyl)-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-(2-bromobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2-(2-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2-(3-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2-(4-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2-(difluoromethoxy)-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-acetyl-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-acetyl-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
-
2-amino-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-bromo-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-bromo-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
-
2-butoxy-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-butyl-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2-chloro-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-chloro-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-3-fluorobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-3-methylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-(methylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-ethylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-ethynylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-fluorobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-hydroxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-iodobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-methoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-methylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-nitrobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(1-methylethoxy)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(1H-pyrrol-1-yl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(ethylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(methylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(methylsulfonyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(phenylcarbonyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(propylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(trifluoromethoxy)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-ethoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-ethylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-fluorobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-iodobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-methoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-methylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-nitrobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-phenoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-propoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-propylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(1-methylethoxy)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(ethylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(methylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(phenylcarbonyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(propylsulfanyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(trifluoromethyl)benzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-ethoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-fluorobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-iodobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-methoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-methylbenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-nitrobenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-phenoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-propoxybenzoic acid
-
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
-
3-[(4,6-dimethoxypyrimidin-2-yl)oxy]biphenyl-2-carboxylic acid
-
5-amino-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
5-benzyl-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
5-bromo-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
5-chloro-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
5-cyano-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
-
8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
leucine
feedback inhibition
penoxsulam
95% inhibition after 40 min at 0.002 mM
thiencarbazone methyl
94% inhibition after 60 min at 0.002 mM
valine
feedback inhibition, the inhibition by valine is uniquely in fungi reversed by MgATP
isoleucine
-
feedback inhibition
L-valine
-
not inhibitory for catalytic subunit alone, inhibitory for catalytic subunit plus small subunit
leucine
-
feedback inhibition
thiencarbazone methyl
-
-
valine
-
feedback inhibition
valine
-
feedback inhibition, reversible by MgATP2-
additional information
molecular docking of inhibitor molecules to the enzyme crystal structure, 3D-QSAR modeling, overview
-
additional information
-
feedback inhibition takes place in the holoenzyme containing the regulatory and the catalytic subunits. The branched-chain amino acids are believed to bind only to the regulatory subunit and inhibit the enzyme
-
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3.62
2-(2-bromobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2.1
2-(2-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2.16
2-(3-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
2.03
2-(4-chlorobenzyl)-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
4.41
2-butyl-8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
7.89
8-[(4,6-dimethoxypyrimidin-2-yl)oxy]-4-methylphthalazin-1(2H)-one
-
0.16
L-valine
-
catalytic subunit plus small subunit, pH 7.0, 30°C
additional information
additional information
-
-
0.003
valine
-
pH 7.0, 30°C, mutant L222A
0.006
valine
-
pH 7.0, 30°C, mutant K218A
0.0156
valine
-
pH 7.0, 30°C, mutant S212A
0.0162
valine
-
pH 7.0, 30°C, mutant L177A
0.02
valine
-
pH 7.0, 30°C, mutant H205A
0.0245
valine
-
pH 7.0, 30°C, mutant R216A
0.026
valine
-
pH 7.0, 30°C, mutant F204A
0.039
valine
-
pH 7.0, 30°C, mutant P206A
0.058
valine
-
pH 7.0, 30°C, mutant H181A
0.177
valine
-
pH 7.0, 30°C, wild-type enzyme
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0.00603
2,3-dichloro-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00568
2-(1,1-dihydroxyethyl)-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00719
2-(difluoromethoxy)-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00684
2-acetyl-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00714
2-acetyl-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
Saccharomyces cerevisiae
-
0.007
2-amino-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00782
2-bromo-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.0074
2-bromo-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
Saccharomyces cerevisiae
-
0.00621
2-butoxy-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00762
2-chloro-6-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00749
2-chloro-6-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
Saccharomyces cerevisiae
-
0.00633
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-3-fluorobenzoic acid
Saccharomyces cerevisiae
-
0.00539
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-3-methylbenzoic acid
Saccharomyces cerevisiae
-
0.00432
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-(methylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00459
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-ethylbenzoic acid
Saccharomyces cerevisiae
-
0.0047
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-ethynylbenzoic acid
Saccharomyces cerevisiae
-
0.00627
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-fluorobenzoic acid
Saccharomyces cerevisiae
-
0.0072
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-hydroxybenzoic acid
Saccharomyces cerevisiae
-
0.00505
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-iodobenzoic acid
Saccharomyces cerevisiae
-
0.0046
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-methoxybenzoic acid
Saccharomyces cerevisiae
-
0.00503
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-methylbenzoic acid
Saccharomyces cerevisiae
-
0.0039
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-5-nitrobenzoic acid
Saccharomyces cerevisiae
-
0.00573
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(1-methylethoxy)benzoic acid
Saccharomyces cerevisiae
-
0.00828
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(1H-pyrrol-1-yl)benzoic acid
Saccharomyces cerevisiae
-
0.00711
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(ethylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00757
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(methylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00587
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(methylsulfonyl)benzoic acid
Saccharomyces cerevisiae
-
0.00567
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(phenylcarbonyl)benzoic acid
Saccharomyces cerevisiae
-
0.00629
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(propylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00696
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-(trifluoromethoxy)benzoic acid
Saccharomyces cerevisiae
-
0.00705
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-ethoxybenzoic acid
Saccharomyces cerevisiae
-
0.00657
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-ethylbenzoic acid
Saccharomyces cerevisiae
-
0.0073
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-fluorobenzoic acid
Saccharomyces cerevisiae
-
0.00766
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-iodobenzoic acid
Saccharomyces cerevisiae
-
0.00736
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-methoxybenzoic acid
Saccharomyces cerevisiae
-
0.00689
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-methylbenzoic acid
Saccharomyces cerevisiae
-
0.00664
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-nitrobenzoic acid
Saccharomyces cerevisiae
-
0.0077
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-phenoxybenzoic acid
Saccharomyces cerevisiae
-
0.00624
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-propoxybenzoic acid
Saccharomyces cerevisiae
-
0.00589
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]-6-propylbenzoic acid
Saccharomyces cerevisiae
-
0.00664
2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00488
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(1-methylethoxy)benzoic acid
Saccharomyces cerevisiae
-
0.00667
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(ethylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00768
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(methylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00519
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(phenylcarbonyl)benzoic acid
Saccharomyces cerevisiae
-
0.00572
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(propylsulfanyl)benzoic acid
Saccharomyces cerevisiae
-
0.00602
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-(trifluoromethyl)benzoic acid
Saccharomyces cerevisiae
-
0.0067
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-ethoxybenzoic acid
Saccharomyces cerevisiae
-
0.00767
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-fluorobenzoic acid
Saccharomyces cerevisiae
-
0.00699
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-iodobenzoic acid
Saccharomyces cerevisiae
-
0.00705
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-methoxybenzoic acid
Saccharomyces cerevisiae
-
0.00753
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-methylbenzoic acid
Saccharomyces cerevisiae
-
0.00669
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-nitrobenzoic acid
Saccharomyces cerevisiae
-
0.0056
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-phenoxybenzoic acid
Saccharomyces cerevisiae
-
0.00614
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]-6-propoxybenzoic acid
Saccharomyces cerevisiae
-
0.0068
2-[(4,6-dimethoxypyrimidin-2-yl)sulfanyl]benzoic acid
Saccharomyces cerevisiae
-
0.0078
3-[(4,6-dimethoxypyrimidin-2-yl)oxy]biphenyl-2-carboxylic acid
Saccharomyces cerevisiae
-
0.00609
5-amino-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00358
5-benzyl-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.0045
5-bromo-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00535
5-chloro-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
0.00371
5-cyano-2-[(4,6-dimethoxypyrimidin-2-yl)oxy]benzoic acid
Saccharomyces cerevisiae
-
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A117D
naturally occuring mutation
A117E
naturally occuring mutation
A117F
naturally occuring mutation
A117H
naturally occuring mutation
A117I
naturally occuring mutation
A117K
naturally occuring mutation
A117L
naturally occuring mutation
A117M
naturally occuring mutation
A117N
naturally occuring mutation
A117P
naturally occuring mutation
A117Q
naturally occuring mutation
A117R
naturally occuring mutation
A117S
naturally occuring mutation
A117T
naturally occuring mutation
A117V
naturally occuring mutation
A117W
naturally occuring mutation
A117Y
naturally occuring mutation
A200C
naturally occuring mutation
A200D
naturally occuring mutation
A200E
naturally occuring mutation
A200R
naturally occuring mutation
A200T
naturally occuring mutation
A200V
naturally occuring mutation
A200W
naturally occuring mutation
A200Y
naturally occuring mutation
A26V
naturally occuring mutation
D379E
naturally occuring mutation
D379G
naturally occuring mutation
D379N
naturally occuring mutation
D379P
naturally occuring mutation
D379S
naturally occuring mutation
D379V
naturally occuring mutation
D379W
naturally occuring mutation
F590C
naturally occuring mutation
F590G
naturally occuring mutation
F590L
naturally occuring mutation
F590N
naturally occuring mutation
F590R
naturally occuring mutation
G116N
naturally occuring mutation
G116S
naturally occuring mutation
K251D
naturally occuring mutation
K251E
naturally occuring mutation
K251N
naturally occuring mutation
K251P
naturally occuring mutation
K251T
naturally occuring mutation
M354C
naturally occuring mutation
M354K
naturally occuring mutation
M354V
naturally occuring mutation
P192A
naturally occuring mutation
P192E
naturally occuring mutation
P192L
naturally occuring mutation
P192Q
naturally occuring mutation
P192R
naturally occuring mutation
P192S
naturally occuring mutation
P192V
naturally occuring mutation
P192W
naturally occuring mutation
P192Y
naturally occuring mutation
V583A
naturally occuring mutation
V583C
naturally occuring mutation
V583N
naturally occuring mutation
V583Y
naturally occuring mutation
V99M
naturally occuring mutation
W586A
naturally occuring mutation
W586C
naturally occuring mutation
W586E
naturally occuring mutation
W586G
naturally occuring mutation
W586H
naturally occuring mutation
W586I
naturally occuring mutation
W586K
naturally occuring mutation
W586L
naturally occuring mutation
W586N
naturally occuring mutation
W586S
naturally occuring mutation
W586V
naturally occuring mutation
F204A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
H181A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
H205A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
H219A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
K218A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
L177A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
L222A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
P206A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
R216A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
S212A
-
site-directed mutagenesis, the mutant shows reduced stimulation by MgATP2- and decreased Ki with valine compared to the wild-type enzyme
additional information
identification and phenotypes of herbicide-resistant mutant enzymes, overview
additional information
-
expression of an Ilv2 variant that lacks the N-terminal mitochondrial targeting sequence leads to highly elevated diacetyl levels comparable to a petite strain. Expression of a mutant allele of the gamma-subunit of the F1-ATPase, ATP3-5, could be an attractive way to reduce diacetyl formation by petite strains
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Ryan, E.D.; Kohlhaw, G.B.
Subcellular localization of isoleucine-valine biosynthetic enzymes in yeast
J. Bacteriol.
120
631-637
1974
Saccharomyces cerevisiae
brenda
Poulsen, C.; Stougaard, P.
Purification and properties of Saccharomyces cerevisiae acetolactate synthase from recombinant Escherichia coli
Eur. J. Biochem.
185
433-439
1989
Saccharomyces cerevisiae
brenda
Pang, S.S.; Duggleby, R.G.
Expression, purification, characterization, and reconstitution of the large and small subunits of yeast acetohydroxyacid synthase
Biochemistry
38
5222-5231
1999
Saccharomyces cerevisiae
brenda
Lee, Y.T.; Duggleby, R.G.
Mutations in the regulatory subunit of yeast acetohydroxyacid synthase affect its activation by MgATP
Biochem. J.
395
331-336
2006
Saccharomyces cerevisiae
brenda
McCourt, J.A.; Duggleby, R.G.
How an enzyme answers multiple-choice questions
Trends Biochem. Sci.
30
222-225
2005
Escherichia coli, Saccharomyces cerevisiae (P07342)
brenda
He, Y.Z.; Li, Y.X.; Zhu, X.L.; Xi, Z.; Niu, C.; Wan, J.; Zhang, L.; Yang, G.F.
Rational design based on bioactive conformation analysis of pyrimidinylbenzoates as acetohydroxyacid synthase inhibitors by integrating molecular docking, CoMFA, CoMSIA, and DFT calculations
J. Chem. Inf. Model.
47
2335-2344
2007
Saccharomyces cerevisiae (P07342)
brenda
Kingsbury, J.M.; McCusker, J.H.
Cytocidal amino acid starvation of Saccharomyces cerevisiae and Candida albicans acetolactate synthase (ilv2{Delta}) mutants is influenced by the carbon source and rapamycin
Microbiology
156
929-939
2009
Saccharomyces cerevisiae, Candida albicans
brenda
Duggleby, R.G.; McCourt, J.A.; Guddat, L.W.
Structure and mechanism of inhibition of plant acetohydroxyacid synthase
Plant Physiol. Biochem.
46
309-324
2008
Arabidopsis thaliana, Brassica napus, Escherichia coli, Helianthus annuus, Nicotiana tabacum, Nitrosomonas europaea, Salmonella enterica subsp. enterica serovar Typhimurium, Thermotoga maritima, Saccharomyces cerevisiae (P07342), Gossypium hirsutum (Q42768)
brenda
Dasari, S.; Koelling, R.
Cytosolic localization of acetohydroxyacid synthase Ilv2 and its impact on diacetyl formation during beer fermentation
Appl. Environ. Microbiol.
77
727-731
2011
Saccharomyces cerevisiae
brenda
Gokhale, K.; Tilak, B.
Mechanisms of bacterial acetohydroxyacid synthase (AHAS) and specific inhibitors of Mycobacterium tuberculosis AHAS as potential drug candidates against tuberculosis
Curr. Drug Targets
16
689-699
2015
Saccharomyces cerevisiae, Mycobacterium tuberculosis, Mycobacterium tuberculosis (P9WG41 and P9WKJ3), Pseudomonas aeruginosa, Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli (P00892 and P0ADG1), Escherichia coli (P00893 and P00894), Escherichia coli (P08142 and P0ADF8), Mycobacterium tuberculosis H37Rv (P9WG41 and P9WKJ3)
brenda
Lee, W.; Seo, S.; Bae, Y.; Nan, H.; Jin, Y.; Seo, J.
Isobutanol production in engineered Saccharomyces cerevisiae by overexpression of 2-ketoisovalerate decarboxylase and valine biosynthetic enzymes
Bioprocess Biosyst. Eng.
35
1467-1475
2012
Saccharomyces cerevisiae (P07342), Saccharomyces cerevisiae
brenda
Lonhienne, T.; Garcia, M.D.; Guddat, L.W.
The role of a FAD cofactor in the regulation of acetohydroxyacid synthase by redox signaling molecules
J. Biol. Chem.
292
5101-5109
2017
Saccharomyces cerevisiae
brenda
Lonhienne, T.; Garcia, M.; Noble, C.; Harmer, J.; Fraser, J.; Williams, C.; Guddat, L.
High resolution crystal structures of the acetohydroxyacid synthase-pyruvate complex provide new insights into its catalytic mechanism
ChemistrySelect
2
11981-11988
2017
Saccharomyces cerevisiae (P07342)
-
brenda
Garcia, M.D.; Wang, J.G.; Lonhienne, T.; Guddat, L.W.
Crystal structure of plant acetohydroxyacid synthase, the target for several commercial herbicides
FEBS J.
284
2037-2051
2017
Saccharomyces cerevisiae, Arabidopsis thaliana (P17597), Arabidopsis thaliana
brenda
Lonhienne, T.; Garcia, M.D.; Pierens, G.; Mobli, M.; Nouwens, A.; Guddat, L.W.
Structural insights into the mechanism of inhibition of AHAS by herbicides
Proc. Natl. Acad. Sci. USA
115
E1945-E1954
2018
Saccharomyces cerevisiae (P07342), Arabidopsis thaliana (P17597)
brenda