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Information on EC 2.2.1.11 - 6-deoxy-5-ketofructose 1-phosphate synthase and Organism(s) Methanocaldococcus jannaschii and UniProt Accession Q58980

for references in articles please use BRENDA:EC2.2.1.11
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EC Tree
IUBMB Comments
The enzyme plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids. The enzyme can also catalyse the reaction of EC 4.1.2.13, fructose-bisphosphate aldolase.
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Methanocaldococcus jannaschii
UNIPROT: Q58980
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The taxonomic range for the selected organisms is: Methanocaldococcus jannaschii
The expected taxonomic range for this enzyme is: Bacteria, Archaea
Synonyms
DKFP synthase, MJ1585, MM0714, MMP0293, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DKFP synthase
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MJ1585
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SYSTEMATIC NAME
IUBMB Comments
2-oxopropanal:D-fructose 1,6-bisphosphate glycerone-phosphotransferase
The enzyme plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids. The enzyme can also catalyse the reaction of EC 4.1.2.13, fructose-bisphosphate aldolase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
methylglyoxal + D-fructose 1,6-bisphosphate
D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
show the reaction diagram
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?
methylglyoxal + D-fructose 1-phosphate
D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
show the reaction diagram
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?
additional information
?
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the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
methylglyoxal + D-fructose 1,6-bisphosphate
D-glyceraldehyde 3-phosphate + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
show the reaction diagram
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-
-
?
methylglyoxal + D-fructose 1-phosphate
D-glyceraldehyde + 1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate
show the reaction diagram
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-
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?
additional information
?
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the enzyme catalyzes the condensation of methylglyoxal with a dihydroxyacetone-phosphate fragment, which is derived from fructose 1-phosphate and/or fructose 1,6-bisphosphate, generating 6-deoxy-5-ketofructose 1-phosphate
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-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene MJ1585
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is required in the biosynthetic pathway for creating 6-deoxy-5-ketofructose 1-phosphate, DKFP, a precursor sugar for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii. This pathway is not leading via the nucleoside diphosphate mannose pathway nor a pathway involving nucleoside diphosphate derivatives of fructose 1-phosphate, fructose 2-phosphate, or fructose 1,6-bisphosphate, but methylglyoxal and a fructose 1-phosphate- or fructose 1,6-bisphosphate-derived dihydroxyacetone phosphate fragment are key intermediates in DKFP production, detailed overview. The stereochemistry of the aldolase reaction is consistent with MJ1585 also functioning as the fructose 1,6-bisphosphate aldolase, EC 4.1.2.13, used in glycolysis. The MJ1585 aldolase does not catalyze the reaction of DL-lactaldehyde with fructose 1-phosphate, fructose 1,6-phosphate, or DHAP to form 6-deoxyallulose 1-phosphate or fuculose 1-phosphate
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene MJ1585, expression in Escherichia coli using primersforward 5'-GGTCATATGGGGATTTTTATG-3' and reverse 5'-GCTGGATCCTTATTTCCTATCTC-3'
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
White, R.H.; Xu, H.
Methylglyoxal is an intermediate in the biosynthesis of 6-deoxy-5-ketofructose-1-phosphate: a precursor for aromatic amino acid biosynthesis in Methanocaldococcus jannaschii
Biochemistry
45
12366-12379
2006
Methanocaldococcus jannaschii (Q58980)
Manually annotated by BRENDA team