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Information on EC 2.2.1.1 - transketolase and Organism(s) Mus musculus and UniProt Accession P40142

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EC Tree
     2 Transferases
         2.2 Transferring aldehyde or ketonic groups
             2.2.1 Transketolases and transaldolases
                2.2.1.1 transketolase
IUBMB Comments
A thiamine-diphosphate protein. Wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO2 and R-CHOH-CO-CH2OH. The enzyme from the bacterium Alcaligenes faecalis shows high activity with D-erythrose 4-phosphate as acceptor.
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This record set is specific for:
Mus musculus
UNIPROT: P40142
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
transketolase, tktl1, transketolase a, transketolase-like 1, tktl-1, transketolase-like-1, tktl2, transketolase-like enzyme 1, transketolase-like-2, glycolaldehydetransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycolaldehydetransferase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
keto group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
sedoheptulose-7-phosphate:D-glyceraldehyde-3-phosphate glycolaldehydetransferase
A thiamine-diphosphate protein. Wide specificity for both reactants, e.g. converts hydroxypyruvate and R-CHO into CO2 and R-CHOH-CO-CH2OH. The enzyme from the bacterium Alcaligenes faecalis shows high activity with D-erythrose 4-phosphate as acceptor.
CAS REGISTRY NUMBER
COMMENTARY hide
9014-48-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-ribose 5-phosphate + D-xylulose 5-phosphate
sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
thiamine diphosphate
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thiamine diphosphate
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
3-(6-methyl-2-amino-pyridin-3-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium chloride hydrochloride
analog of thiamine, potent transketolase inhibitor but suffers from poor pharmacokinetics due to high clearance and Cmax linked toxicity. Efficient way of improving the pharmacokinetic profile is to prepare oxidized prodrugs which are slowly reduced in vivo yielding longer, sustained blood levels of the drug
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.33
D-ribose 5-phosphate
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cosubstrate D-xylulose 5-phosphate
0.12
D-xylulose 5-phosphate
-
(+ D-ribose 5-phosphate)
0.007
thiamine diphosphate
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Balb-c mice
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
thiamine-deficinet diet reduces transketolase activity in both cortex and hippocampus, without significantly affecting the activities of pyruvate dehydrogenase, alpha-ketoglutamate dehydrogenase, and glucose 6-phosphate dehydrogenase
Manually annotated by BRENDA team
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thiamine-deficient diet reduces transketolase activity in both cortex and hippocampus, without significantly affecting the activities of pyruvate dehydrogenase, alpha-ketoglutamate dehydrogenase, and glucose 6-phosphate dehydrogenase
Manually annotated by BRENDA team
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enzyme is highly expressed at 2 hours of spontaneous maturation in oocytes
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
TKT_MOUSE
623
0
67630
Swiss-Prot
other Location (Reliability: 2)
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
drug development
ring-opened analogs of the transketolase inhibitor 'N30-pyridyl thiamine'; 3-(6-methyl-2-amino-pyridin-3-ylmethyl)-5-(2-hydroxy-ethyl)-4-methyl-thiazol-3-ium chloride hydrochloride are effectively transformed in vivo to the active thiazoliums, whereby the effectiveness of this transformation is dependent on the sulfur moiety. The pharmacokinetic properties of the transketolase inhibitor are improved, the Cmax is reduced, thus avoiding toxicity, and higher distribution and exposure correlate in vivo with a stronger and longer inhibition of blood transketolase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Blass, J.P.; Piacentini, S.; Boldizsar, E.; Baker, A.
Kinetic studies of mouse brain transketolase
J. Neurochem.
39
729-733
1982
Mus musculus
Manually annotated by BRENDA team
Schenk, G.; Duggleby, R.G.; Nixon, P.F.
Properties and functions of the thiamin diphosphate dependent enzyme transketolase
Int. J. Biochem. Cell Biol.
30
1297-1318
1998
Cyberlindnera jadinii, Oryctolagus cuniculus, Escherichia coli, Homo sapiens, Mus musculus, Rattus norvegicus, Saccharomyces pastorianus, Spinacia oleracea, Sus scrofa, Saccharomyces cerevisiae (P23254), Saccharomyces cerevisiae
Manually annotated by BRENDA team
Le Huerou, Y.; Gunawardana, I.; Thomas, A.A.; Boyd, S.A.; de Meese, J.; Dewolf, W.; Gonzales, S.S.; Han, M.; Hayter, L.; Kaplan, T.; Lemieux, C.; Lee, P.; Pheneger, J.; Poch, G.; Romoff, T.T.; Sullivan, F.; Weiler, S.; Wright, S.K.; Lin, J.
Prodrug thiamine analogs as inhibitors of the enzyme transketolase
Bioorg. Med. Chem. Lett.
18
505-508
2008
Mus musculus (P40142), Mus musculus Balb-c (P40142)
Manually annotated by BRENDA team
Zhao, Y.; Pan, X.; Zhao, J.; Wang, Y.; Peng, Y.; Zhong, C.
Decreased transketolase activity contributes to impaired hippocampal neurogenesis induced by thiamine deficiency
J. Neurochem.
111
537-546
2009
Mus musculus
Manually annotated by BRENDA team
Kim, Y.; Kim, E.Y.; Seo, Y.M.; Yoon, T.K.; Lee, W.S.; Lee, K.A.
Function of the pentose phosphate pathway and its key enzyme, transketolase, in the regulation of the meiotic cell cycle in oocytes
Clin. Exp. Reprod. Med.
39
58-67
2012
Mus musculus
Manually annotated by BRENDA team