Information on EC 2.10.1.1 - molybdopterin molybdotransferase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

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EC NUMBER
COMMENTARY hide
2.10.1.1
-
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RECOMMENDED NAME
GeneOntology No.
molybdopterin molybdotransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
adenylyl-molybdopterin + molybdate = molybdenum cofactor + AMP + H2O
show the reaction diagram
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-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
molybdate transfer
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
molybdenum cofactor biosynthesis
Folate biosynthesis
-
-
Metabolic pathways
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-
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SYSTEMATIC NAME
IUBMB Comments
adenylyl-molybdopterin:molybdate molybdate transferase (AMP-forming)
Catalyses the insertion of molybdenum into the ene-dithiol group of molybdopterin. In eukaryotes this reaction is catalysed by the N-terminal domain of a fusion protein whose C-terminal domain catalyses EC 2.7.7.75, molybdopterin adenylyltransferase. Requires divalent cations such as Mg2+ or Zn2+ for activity.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
domain E
SwissProt
Manually annotated by BRENDA team
K-12
SwissProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
adenylyl-molybdopterin + molybdate + 4 H+
molybdenum cofactor + AMP + H2O
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
adenylyl-molybdopterin + molybdate + 4 H+
molybdenum cofactor + AMP + H2O
show the reaction diagram
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mn2+
required, less efficient
Ni2+
required, less efficient
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
glutathione
-
50% inhibition at 0.4 mM
L-cysteine
-
50% inhibition at 0.01 mM
molybdopterin adenylyltransferase
-
in absence of Mg2+ and ATP
-
Na2S
-
50% inhibition at 1.3 mM
sulfate
less efficient inhibition than tungstate
tungstate
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
molybdopterin adenylyltransferase
-
in presence of Mg2+ and ATP
-
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25 - 2.5
molybdate
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PDB
SCOP
CATH
UNIPROT
ORGANISM
Arabidopsis thaliana;
Escherichia coli (strain K12);
Rattus norvegicus;
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D142N
-
high activity in vitro
D228A
-
wild type activity in vitro
D228N
-
wild type activity in vitro
D259A
-
high activity in vitro
D59A
-
high activity in vitro
D59N
-
high activity in vitro
delPhe53-Arg139
-
inactive in vivo, low activity in vitro
E188A
-
wild type activity in vitro
E188Q
-
wild type activity in vitro
K275Q
-
decreased activity in vitro
K279Q
-
low activity in vitro
R137Q
-
decreased activity in vitro
R350A
-
low activity in vitro
S271A
-
decreased activity in vitro
S371W
-
low activity in vitro
T100A
-
decreased activity in vitro
T100W
-
decreased activity in vitro
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Show AA Sequence (1775 entries)
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LINKS TO OTHER DATABASES (specific for EC-Number 2.10.1.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)