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Information on EC 2.1.3.3 - ornithine carbamoyltransferase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WIT9

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IUBMB Comments
The plant enzyme also catalyses the reactions of EC 2.1.3.6 putrescine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine [(4-aminobutyl)guanidine] and ornithine into putrescine and citrulline, respectively.
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This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WIT9
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The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
oct, ornithine transcarbamylase, ornithine carbamoyltransferase, otcase, ornithine transcarbamoylase, carbamoyltransferase, ornithine carbamyl transferase, ornithine carbamyltransferase, catabolic ornithine carbamoyltransferase, rotcase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoyltransferase, ornithine
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carbamylphosphate-ornithine transcarbamylase
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citrulline phosphorylase
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L-ornithine carbamoyltransferase
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L-ornithine carbamyltransferase
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L-ornithine transcarbamylase
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ornithine carbamyltransferase
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ornithine transcarbamoylase
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OTC
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
carbamoyl group transfer
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PATHWAY SOURCE
PATHWAYS
BRENDA
-
-, -, -, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
carbamoyl-phosphate:L-ornithine carbamoyltransferase
The plant enzyme also catalyses the reactions of EC 2.1.3.6 putrescine carbamoyltransferase, EC 2.7.2.2 carbamate kinase and EC 3.5.3.12 agmatine deiminase, thus acting as putrescine synthase, converting agmatine [(4-aminobutyl)guanidine] and ornithine into putrescine and citrulline, respectively.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-69-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + L-ornithine
phosphate + citrulline
show the reaction diagram
catalyzes the sixth step in arginine biosynthesis
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-
r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
carbamoyl phosphate + L-ornithine
phosphate + citrulline
show the reaction diagram
catalyzes the sixth step in arginine biosynthesis
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-
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-norvaline
competitive
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
33000
3 * 33000, crystal structure analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
crystal structure analysis, possibly a crystallization artifact
trimer
3 * 33000, crystal structure analysis
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, crystal structures of Mtb OTC in orthorhombic and hexagonal form hexagonal form crystals complexed with carbamoyl phosphate and L-norvaline
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
ornithine carbamyltransferase is present in urine of patients with pulmonary tuberculosis. Recombinant ornithine carboamyltransferase produced in Escherichia coli is recognized by immunoglobulin G antibodies from patients with active tuberculosis but not by IgG from uninfected healthy subjects. Protein is strongly recognized by peripheral blood mononuclear cells from both healthy tuberculin purified protein derivative-positive individuals and patients with pulmonary tuberculosis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sankaranarayanan, R.; Cherney, M.M.; Cherney, L.T.; Garen, C.R.; Moradian, F.; James, M.N.
The crystal structures of ornithine carbamoyltransferase from Mycobacterium tuberculosis and its ternary complex with carbamoyl phosphate and L-norvaline reveal the enzymes catalytic mechanism
J. Mol. Biol.
375
1052-1063
2008
Mycobacterium tuberculosis (P9WIT9), Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv (P9WIT9)
Manually annotated by BRENDA team
Napolitano, D.R.; Pollock, N.; Kashino, S.S.; Rodrigues, V.; Campos-Neto, A.
Identification of Mycobacterium tuberculosis ornithine carboamyltransferase in urine as a possible molecular marker of active pulmonary tuberculosis
Clin. Vaccine Immunol.
15
638-643
2008
Mycobacterium tuberculosis (P9WIT9), Mycobacterium tuberculosis H37Rv (P9WIT9)
Manually annotated by BRENDA team