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Information on EC 2.1.2.1 - glycine hydroxymethyltransferase and Organism(s) Methanosarcina barkeri and UniProt Accession Q46A52

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EC Tree
IUBMB Comments
A pyridoxal-phosphate protein. Also catalyses the reaction of glycine with acetaldehyde to form L-threonine, and with 4-trimethylammoniobutanal to form 3-hydroxy-N6,N6,N6-trimethyl-L-lysine.
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Methanosarcina barkeri
UNIPROT: Q46A52
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Word Map
The taxonomic range for the selected organisms is: Methanosarcina barkeri
The enzyme appears in selected viruses and cellular organisms
Synonyms
serine hydroxymethyltransferase, shmt2, shmt1, serine hydroxymethyl transferase, serine transhydroxymethylase, serine hydroxymethyltransferase 2, mitochondrial serine hydroxymethyltransferase, serine hydroxymethyltransferase 1, bsshmt, pvshmt, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
serine hydroxymethyltransferase
-
serine:H4F hydroxymethyltransferase
-
serine:tetrahydrofolate hydroxymethyltransferase
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L-serine hydroxymethyltransferase
-
-
-
-
serine hydroxymethylase hydroxymethyltransferase, serine
-
-
-
-
serine hydroxymethyltransferase
-
-
-
-
serine transhydroxymethylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxymethyl group transfer
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
5,10-methylenetetrahydrofolate:glycine hydroxymethyltransferase
A pyridoxal-phosphate protein. Also catalyses the reaction of glycine with acetaldehyde to form L-threonine, and with 4-trimethylammoniobutanal to form 3-hydroxy-N6,N6,N6-trimethyl-L-lysine.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-83-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(6S)-tetrahydrofolate + L-serine
5,10-methylenetetrahydrofolate + glycine + H2O
show the reaction diagram
the enzyme also catalyzes the formation of methylene-tetrahydromethanopterin from tetrahydromethanopterin and L-serine, albeit with a catalytic efficiency which is less than 1% of that with (6S)-tetrahydrofolate as substrate. The catalytic efficiency with methylene-tetrahydrosarcinapterin as substrate is even lower
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-
?
tetrahydrofolate + L-serine
5,10-methylenetetrahydrofolate + glycine + H2O
show the reaction diagram
-
-
-
?
additional information
?
-
the enzyme also catalyzes the tetrahydrofolate-independent retroaldol cleavage of L-allo-threonine and L-threonine to glycine and acetaldehyde
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pyridoxal 5'-phosphate
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
tetrahydrofolate
at concentrations above 0.015 mM, substrate inhibition is observed
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002
(6S)-tetrahydrofolate
pH 7.2, 37°C
0.2 - 0.29
L-serine
0.086
tetrahydrofolate
pH and temperature not specified in the publication
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.5
pH 7.2, 37°C, extract from Escherichia coli cells carrying the expression vector
4.3
pH 7.2, 37°C, purified enzyme, recombinant
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
gel filtration
43000
2 * 43000, SDS-PAGE
45047
2 * 45047, calculated from sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homodimer
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterologously overproduced in Escherichia coli without a His6-tag
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Buchenau, B.; Thauer, R.K.
Tetrahydrofolate-specific enzymes in Methanosarcina barkeri and growth dependence of this methanogenic archaeon on folic acid or p-aminobenzoic acid
Arch. Microbiol.
182
313-325
2004
Methanosarcina barkeri (Q46A52), Methanosarcina barkeri DSM 804 (Q46A52)
Manually annotated by BRENDA team
Lin, Z.; Sparling, R.
Investigation of serine hydroxymethyltransferase in methanogens
Can. J. Microbiol.
44
652-656
1998
Methanolobus tindarius, Methanosarcina barkeri (Q46A52), Methanosarcina barkeri, Methanosarcina barkeri DSM 804 (Q46A52), Methanospirillum hungatei, Methanospirillum hungatei GP1, Methanothrix soehngenii
Manually annotated by BRENDA team