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Information on EC 2.1.1.B84 - cephaline 6'-O-methyltransferase
for references in articles please use BRENDA:EC2.1.1.B84preliminary BRENDA-supplied EC number
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2.1.1.B84 cephaline 6'-O-methyltransferaseSpecify your search results
The expected taxonomic range for this enzyme is: Carapichea ipecacuanha
Synonyms
CiOMT1, IpeOMT1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IpeOMT1
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + cephaeline = S-adenosyl-L-homocysteine + emetine
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:cephaeline 6'-O-methyltransferase
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (R)-norprotosinomenine
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (R,S)-isococlaurine
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (R,S)-isoorientaline
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (R,S)-norcoclaurine
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (R,S)-protosinomenine
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (S)-4'-O-methyllaudanosoline
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + (S)-norprotosinomenine
S-adenosyl-L-homocysteine + ?
-
-
-
?
S-adenosyl-L-methionine + 1alpha-(S)-N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylisoipecoside
S-adenosyl-L-methionine + 7-O-methyl-N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6,7-O,O-dimethyl-N-deacetylisoipecoside
-
-
-
?
S-adenosyl-L-methionine + N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylipecoside
-
-
-
?
S-adenosyl-L-methionine + 1alpha-(S)-N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylisoipecoside
-
-
-
-
?
S-adenosyl-L-methionine + 1alpha-(S)-N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylisoipecoside
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the iminium cation, one of the spontaneous reaction products of 6-O-methyl-N-deacetylisoipecoside aglycon, might be an intermediate of emetine biosynthesis
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-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
-
-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
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-
-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
the enzyme is involved in emetine biosynthesis. It does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline, the preceding step in the biosynthesis of emetine
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-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
6'-O-methylation
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-
?
additional information
?
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IpeOMT1 does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline. IpeOMT1 shows broad substrate specificity towards the intermediates in emetine biosynthesis. IpeOMT1 dominantly catalyzes 6-O-methylation of the 6,7-dihydroxy- and 6-hydroxy-7-methoxyisoquinolines. The regional substrate specificity at the last step of emetine biosynthesis is restricted to 6'-O-methylation
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-
?
additional information
?
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the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
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-
?
additional information
?
-
substrate specificity of CiOMT2, overview
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-
?
additional information
?
-
IpeOMT1 does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline. IpeOMT1 shows broad substrate specificity towards the intermediates in emetine biosynthesis. IpeOMT1 dominantly catalyzes 6-O-methylation of the 6,7-dihydroxy- and 6-hydroxy-7-methoxyisoquinolines. The regional substrate specificity at the last step of emetine biosynthesis is restricted to 6'-O-methylation
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-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 1alpha-(S)-N-deacetylisoipecoside
S-adenosyl-L-homocysteine + 6-O-methyl-N-deacetylisoipecoside
-
the iminium cation, one of the spontaneous reaction products of 6-O-methyl-N-deacetylisoipecoside aglycon, might be an intermediate of emetine biosynthesis
-
-
?
additional information
?
-
the enzyme is also active on 7'-O-demethylcephaeline forming cephaeline, overview
-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
-
-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
-
-
-
-
?
S-adenosyl-L-methionine + cephaeline
S-adenosyl-L-homocysteine + emetine
the enzyme is involved in emetine biosynthesis. It does not catalyze the 7'-O-methylation of 7'-O-demethylcephaeline, the preceding step in the biosynthesis of emetine
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.042
S-adenosyl-L-homocysteine
pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside
0.097
S-adenosyl-L-methionine
pH 6.8, 30°C, recombinant CiOMT1
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0071
S-adenosyl-L-homocysteine
pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.169
S-adenosyl-L-homocysteine
pH 7.5, 30°C, cosubstrate: N-deacetylisoipecoside
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
metabolism
CiOMT2 catalyzes the penultimate step, 7-O'-methylation, in emetine biosynthesis, the recombinant CiOMT2 also the last step, synthesis of emetine by 6'-O-methylation, pathway overview
metabolism
-
OMT1 catalyzes a step in the biosynthesis of Ipecac alkaloids, pathways overview
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
OMT2, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21(DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Cheong, B.E.; Takemura, T.; Yoshimatsu, K.; Sato, F.
Molecular cloning of an O-methyltransferase from adventitious roots of Carapichea ipecacuanha
Biosci. Biotechnol. Biochem.
75
107-113
2011
Carapichea ipecacuanha (E7FL15)
brenda
Nomura, T.; Kutchan, T.M.
Three new O-methyltransferases are sufficient for all O-methylation reactions of ipecac alkaloid biosynthesis in root culture of Psychotria ipecacuanha
J. Biol. Chem.
285
7722-7738
2010
Carapichea ipecacuanha (D3KY98), Carapichea ipecacuanha
brenda
Nomura, T.; Kutchan, T.M.
Is a metabolic enzyme complex involved in the efficient and accurate control of Ipecac alkaloid biosynthesis in Psychotria ipecacuanha?
Plant Signal. Behav.
5
875-877
2010
Carapichea ipecacuanha
brenda
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