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Information on EC 2.1.1.B128 - 28S rRNA (adenine1322-N1)-methyltransferase and Organism(s) Homo sapiens and UniProt Accession O43159

for references in articles please use BRENDA:EC2.1.1.B128
preliminary BRENDA-supplied EC number
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This record set is specific for:
Homo sapiens
UNIPROT: O43159
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Homo sapiens
Reaction Schemes
Synonyms
nucleomethylin, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
m1A large subunit rRNA methyltransferase
-
nucleomethylin
-
ribosomal RNA-processing protein 8
UniProt
additional information
cf. EC 2.1.1.287
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1322 in 28S rRNA
S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + adenine1322 in 28S rRNA
S-adenosyl-L-homocysteine + N1-methyladenine1322 in 28S rRNA
show the reaction diagram
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
NML is primarily localized in the nucleolus
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
the N1-methyladenosine in helix 25.1 of 25/28S rRNA seems to be highly conserved
malfunction
siRNA-mediated depletion of NML is highly efficient at mRNA level, impact of an NML knockdown on m1A1322 methylation in HCT-116 cells. Depletion of NML leads to abnormal and altered nucleolar morphology
physiological function
the human nucleomethylin (NML or RRP8) fails to complement the pre-rRNA processing phenotype and the growth cold sensitivity of Saccharomyces cerevisiae DELTArrp8 mutant strain
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
RRP8_HUMAN
456
0
50715
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene rrp8, sequence comparisons and phylogenetic analysis, quantitative enzyme expression analysis, recombinant expression in Saccharomyces cereviaie Rrp8 deficient mutant strain without complementation by the human Rrp8 enzyme
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sharma, S.; Hartmann, J.; Watzinger, P.; Klepper, A.; Peifer, C.; Koetter, P.; Lafontaine, D.; Entian, K.
A single N1-methyladenosine on the large ribosomal subunit rRNA impacts locally its structure and the translation of key metabolic enzymes
Sci. Rep.
8
11904
2018
Homo sapiens (O43159), Homo sapiens
Manually annotated by BRENDA team