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Information on EC 2.1.1.94 - tabersonine 16-O-methyltransferase and Organism(s) Catharanthus roseus and UniProt Accession B0EXJ8

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.94 tabersonine 16-O-methyltransferase
IUBMB Comments
Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle, Catharanthus roseus.
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This record set is specific for:
Catharanthus roseus
UNIPROT: B0EXJ8
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Word Map
The taxonomic range for the selected organisms is: Catharanthus roseus
The expected taxonomic range for this enzyme is: Catharanthus roseus
Synonyms
16omt, 16-hydroxytabersonine-16-o-methyltransferase, tabersonine 16-o-methyltransferase, 11-o-demethyl-17-o-deacetylvindoline 11-o-methyltransferase, 16-hydroxytabersonine 16-o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
16-hydroxytabersonine 16-O-methyltransferase
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16-hydroxytabersonine-16-O-methyltransferase
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16-hydroxytabersonine-O-methyltransferase
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11-demethyl-17-deacetylvindoline 11-methyltransferase
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-
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11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase
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11-O-demethyl-17-O-deacetylvindoline O-methyltransferase
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-
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methyltransferase, 11-demethyl-17-deacetylvindoline 11-
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-
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S-adenosyl-L-methionine:11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:16-hydroxytabersonine 16-O-methyltransferase
Involved in the biosynthesis of vindoline from tabersonine in the Madagascar periwinkle, Catharanthus roseus.
CAS REGISTRY NUMBER
COMMENTARY hide
100984-95-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 16-hydroxytabersonine
S-adenosyl-L-homocysteine + 16-methoxytabersonine
show the reaction diagram
S-adenosyl-L-methionine + 11-O-demethyl-17-O-deacetylvindoline
S-adenosyl-L-homocysteine + 17-O-deacetylvindoline
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 16-hydroxytabersonine
S-adenosyl-L-homocysteine + 16-methoxytabersonine
show the reaction diagram
S-adenosyl-L-methionine + 11-O-demethyl-17-O-deacetylvindoline
S-adenosyl-L-homocysteine + 17-O-deacetylvindoline
show the reaction diagram
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involved in biosynthesis of the alkaloid vindoline
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-homocysteine
product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0026
16-hydroxytabersonine
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0.0217
S-adenosyl-L-methionine
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.006
S-adenosyl-L-homocysteine
product inhibition
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000417
16-hydroxytabersonine as a substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
assay at
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
specifically located in the epidermis of aerial organs
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HTOMT_CATRO
355
0
39863
Swiss-Prot
other Location (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
using PD-10 column chromatography, Sephadex G150 column chromatography, adenosine-agarose affinity resin and Mono Q anion exchange column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
16-hydroxytabersonine-16-O-methyltransferase is isolated and cloned as a His-tagged fusion protein in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Fahn, W.; Laussermair, E.; Deus-Neumann, B.; Stckigt, J.
Late enzymes of vindoline biosynthesis. S-adenosyl-L-methionine:11-O-demethyl-17-O-deacetylvindoline 11-O-methyltransferase and unspecific acetylesterase
Plant Cell Rep.
4
337-340
1985
Catharanthus roseus
Manually annotated by BRENDA team
Levac, D.; Murata, J.; Kim, W.S.; De Luca, V.
Application of carborundum abrasion for investigating the leaf epidermis: molecular cloning of Catharanthus roseus 16-hydroxytabersonine-16-O-methyltransferase
Plant J.
53
225-236
2008
Catharanthus roseus (B0EXJ8), Catharanthus roseus
Manually annotated by BRENDA team
Guirimand, G.; Guihur, A.; Poutrain, P.; Hericourt, F.; Mahroug, S.; St-Pierre, B.; Burlat, V.; Courdavault, V.
Spatial organization of the vindoline biosynthetic pathway in Catharanthus roseus
J. Plant Physiol.
168
549-557
2010
Catharanthus roseus (B0EXJ8), Catharanthus roseus
Manually annotated by BRENDA team
Pandey, S.S.; Singh, S.; Babu, C.S.; Shanker, K.; Srivastava, N.K.; Shukla, A.K.; Kalra, A.
Fungal endophytes of Catharanthus roseus enhance vindoline content by modulating structural and regulatory genes related to terpenoid indole alkaloid biosynthesis
Sci. Rep.
6
26583
2016
Catharanthus roseus (B0EXJ8)
Manually annotated by BRENDA team
Sun, J.; Zhao, L.; Shao, Z.; Shanks, J.; Peebles, C.A.M.
Expression of tabersonine 16-hydroxylase and 16-hydroxytabersonine-O-methyltransferase in Catharanthus roseus hairy roots
Biotechnol. Bioeng.
115
673-683
2018
Catharanthus roseus (B0EXJ8), Catharanthus roseus
Manually annotated by BRENDA team