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Information on EC 2.1.1.80 - protein-glutamate O-methyltransferase and Organism(s) Pseudomonas putida and UniProt Accession Q88ER1

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.80 protein-glutamate O-methyltransferase
IUBMB Comments
Forms ester groups with L-glutamate residues in a number of membrane proteins.
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This record set is specific for:
Pseudomonas putida
UNIPROT: Q88ER1
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Word Map
The taxonomic range for the selected organisms is: Pseudomonas putida
The enzyme appears in selected viruses and cellular organisms
Synonyms
protein methyltransferase, protein methylase ii, cher1, methyltransferase cher, cher2, protein carboxymethylase, protein carboxymethyltransferase, protein carboxyl-methylase, protein o-methyltransferase, omtf3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MCP methyltransferase I
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MCP methyltransferase II
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methyl-accepting chemotaxis protein methyltransferase II
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methyl-accepting chemotaxis protein O-methyltransferase
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methyltransferase, protein O-
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protein carboxyl-methylase
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protein carboxyl-O-methyltransferase
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protein carboxylmethyltransferase II
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protein carboxymethylase
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protein carboxymethyltransferase
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protein methylase II
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protein methyltransferase II
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protein O-methyltransferase
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protein(aspartate)methyltransferase
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protein(carboxyl)methyltransferase
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S-adenosylmethionine-glutamyl methyltransferase
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S-adenosylmethionine:protein-carboxyl O-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-glutamate O-methyltransferase
Forms ester groups with L-glutamate residues in a number of membrane proteins.
CAS REGISTRY NUMBER
COMMENTARY hide
9055-09-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
show the reaction diagram
substrate for isoform CheR2 only
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-
?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
show the reaction diagram
substrate for isoform CheR2 only
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-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + chemotaxis receptors McpS L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpS L-glutamate methyl ester
show the reaction diagram
substrate for isoform CheR2 only
-
-
?
S-adenosyl-L-methionine + chemotaxis receptors McpT L-glutamate
S-adenosyl-L-homocysteine + chemotaxis receptors McpT L-glutamate methyl ester
show the reaction diagram
substrate for isoform CheR2 only
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-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
isoform CheR2 is essential for chemotaxis
physiological function
isoform CheR1 is required for efficient biofilm formation
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32247
1 * 32247, isoform CheR2, calculated from amino acid sequence
38600
1 * 38600, isoform CheR2, sedimentation equilibrium ultracentrifugation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
48
isoform CheR2 has a melting temperature of 48°C
44
isoform CheR1 has a melting temperature of 44°C
49
isoform CheR3 has a melting temperature of 49°C
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
HisTrap column chromatography, and gel filtration
HisTrap column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli C41(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Garcia-Fontana, C.; Reyes-Darias, J.A.; Munoz-Martinez, F.; Alfonso, C.; Morel, B.; Ramos, J.L.; Krell, T.
High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation
J. Biol. Chem.
288
18987-18999
2013
Pseudomonas putida (Q88ER1), Pseudomonas putida (Q88GG4), Pseudomonas putida (Q88MS8), Pseudomonas putida, Pseudomonas putida KT 2240 (Q88ER1), Pseudomonas putida KT 2240 (Q88GG4), Pseudomonas putida KT 2240 (Q88MS8)
Manually annotated by BRENDA team