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Information on EC 2.1.1.79 - cyclopropane-fatty-acyl-phospholipid synthase
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EC Tree
2.1.1.79 cyclopropane-fatty-acyl-phospholipid synthaseIUBMB Comments
The enzyme adds a methylene group across the 9,10 position of a Delta9-olefinic acyl chain in phosphatidylethanolamine or, more slowly, phosphatidylglycerol or phosphatidylinositol, forming a cyclopropane derivative (cf. EC 2.1.1.16 methylene-fatty-acyl-phospholipid synthase).
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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
cfa synthase, cyclopropane fatty acid synthase, cyclopropane synthase, sfcpa-fas, cyclopropane-fatty-acyl-phospholipid synthase, cyclopropane fatty acid synthetase, c17:cyclopropane synthase, hp0416, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
C17:cyclopropane synthase
Cfa
CFA synthase
cfaB
CFAS
cyclopropane fatty acid synthase
cyclopropane fatty acid synthetase
cyclopropane fatty acyl synthase
cyclopropane synthase
cyclopropane-fatty-acyl-phospholipid synthase
HP0416
synthetase, cyclopropane fatty acid
-
-
-
-
unsaturated-phospholipid methyltransferase
-
-
-
-
CFA synthase
-
-
-
-
CFAS
-
-
-
-
cyclopropane fatty acid synthase
-
-
-
-
cyclopropane fatty acid synthase
-
-
cyclopropane fatty acid synthetase
-
-
-
-
cyclopropane synthase
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
both chemical steps of the enzymatic cyclopropanation, the methyl addition onto the double bond and the deprotonation step, are rate determining
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
-
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:unsaturated-phospholipid methyltransferase (cyclizing)
The enzyme adds a methylene group across the 9,10 position of a Delta9-olefinic acyl chain in phosphatidylethanolamine or, more slowly, phosphatidylglycerol or phosphatidylinositol, forming a cyclopropane derivative (cf. EC 2.1.1.16 methylene-fatty-acyl-phospholipid synthase).
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CAS REGISTRY NUMBER
COMMENTARY
51845-48-8
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
S-adenosyl-L-homocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
reaction takes place via methyl transfer followed by proton loss, rather than by processes that are initiated by proton abstraction from S-adenosyl-L-methionine. Methyl transfer takes place via a tight SN2 transition state
-
-
?
S-adenosyl-L-methionine + cardiolipin
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + phosphatidylcholine
?
-
the enzyme acts primarily on the sn-1 position of + phosphatidylcholine
-
-
?
S-adenosyl-L-methionine + phosphatidylglycerol
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
S-adenosyl-L-methionine + phospholipids
S-adenosyl-L-homocysteine + phospholipid + cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + plasmenylethanolamine
S-adenosyl-L-homocysteine + plasmenylethanolamine cyclopropane fatty acid
Se-adenosyl-L-selenomethionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
Se-adenosyl-L-selenohomocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
-
-
-
?
Te-adenosyl-L-telluromethionine + 1-stearoyl-2-oleoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
Te-adenosyl-L-tellurohomocysteine + 1-stearoyl-2-dihydrosterculoyl-sn-glycero-3-[phospho-rac-(1-glycerol)]
-
-
-
-
?
S-adenosyl-L-homocysteine + phosphatidylcholine
S-adenosyl-L-homocysteine + ?
-
phosphatidylcholine is not a substrate
-
-
?
S-adenosyl-L-homocysteine + phosphatidylcholine
S-adenosyl-L-homocysteine + ?
-
addition of the methylene group to oleic acid occurs at the sn-1 position
-
-
?
S-adenosyl-L-methionine + oleate
S-adenosyl-L-homocysteine + dihydrosterculate
-
-
-
?
S-adenosyl-L-methionine + oleate
S-adenosyl-L-homocysteine + dihydrosterculate
-
-
-
?
S-adenosyl-L-methionine + oleic acid
S-adenosyl-L-homocysteine + dihydrosterculic acid
-
-
-
-
?
S-adenosyl-L-methionine + oleic acid
S-adenosyl-L-homocysteine + dihydrosterculic acid
Limosilactobacillus reuteri ATCC PTA 6475
-
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + phosphatidylethanolamine
S-adenosyl-L-homocysteine + ?
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
phosphatidylethanolamine which contains cyclopropane fatty acids
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
no exchange of the cylopropane methylene protons with the solvent during catalysis. There is a significant intermolecular primary tritium kinetic isotope effect consistent with a partially rate determining deprotonation step
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
reaction is not affected by the order-disorder state of the lipid substrate
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
increased level of cyclopropane fatty acid synthase activity as bacterial cultures enter stationary phase is transient, activity quickly declines to the basal level, the loss of activity is due to proteolytic degradation dependent on expression of the heat shock regulon
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the enzyme acts on sn-2 of phospholipids
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Escherichia coli B / ATCC 11303
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Eubacterium HX
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Lacticaseibacillus casei ATCC 334
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
S-adenosylmethionine levels in conjugation with cyclopropane fatty acid synthase activities regulates cyclopropane fatty acid synthesis in Lactobacillus plantarum
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the cfa locus is an essential component of the acid stress response of Lactococcus lactis MG1363
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the cfa locus is an essential component of the acid stress response of Lactococcus lactis MG1363
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
increase in expression of CFA synthase at early stationary phase is due to the alternative sigma factor RpoS
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
Serratia marcescens contains endogenous lipid substrates which cannot be removed or replaced by simple manipulations
phosphatidylethanolamine which contains cyclopropane fatty acids
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
addition of a methylene group from S-adenosylmethionine to the cis-double bond of monoenoic phospholipid-bound fatty acids
-
-
?
S-adenosyl-L-methionine + plasmenylethanolamine
S-adenosyl-L-homocysteine + plasmenylethanolamine cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + plasmenylethanolamine
S-adenosyl-L-homocysteine + plasmenylethanolamine cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + vaccenic acid
S-adenosyl-L-homocysteine + lactobacillic acid
-
-
-
-
?
S-adenosyl-L-methionine + vaccenic acid
S-adenosyl-L-homocysteine + lactobacillic acid
Limosilactobacillus reuteri ATCC PTA 6475
-
-
-
-
?
additional information
?
-
-
growth conditions that result in an increase in the relative amount of C17:cyclopropane fatty acid yield better survival after lyophilization
-
-
?
additional information
?
-
-
growth conditions that result in an increase in the relative amount of C17:cyclopropane fatty acid yield better survival after lyophilization
-
-
?
additional information
?
-
-
cyclopropanation occurs in the sn-1 and sn-2 positions in lipids, cyclopropanation of phosphatidylcholines and sulfoquinovosyldiacylglycerols occurs more extensively with acidity than with phosphate starvation
-
-
?
additional information
?
-
-
cyclopropanation occurs in the sn-1 and sn-2 positions in lipids, cyclopropanation of phosphatidylcholines and sulfoquinovosyldiacylglycerols occurs more extensively with acidity than with phosphate starvation
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
increased level of cyclopropane fatty acid synthase activity as bacterial cultures enter stationary phase is transient, activity quickly declines to the basal level, the loss of activity is due to proteolytic degradation dependent on expression of the heat shock regulon
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
methylation of unsaturated fatty acid moieties of phospholipids in the phospholipid bilayer
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the enzyme acts on sn-2 of phospholipids
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
Lacticaseibacillus casei ATCC 334
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
S-adenosylmethionine levels in conjugation with cyclopropane fatty acid synthase activities regulates cyclopropane fatty acid synthesis in Lactobacillus plantarum
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the cfa locus is an essential component of the acid stress response of Lactococcus lactis MG1363
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
the cfa locus is an essential component of the acid stress response of Lactococcus lactis MG1363
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
-
-
-
?
S-adenosyl-L-methionine + phospholipid olefinic fatty acid
S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid
-
increase in expression of CFA synthase at early stationary phase is due to the alternative sigma factor RpoS
-
-
?
additional information
?
-
-
growth conditions that result in an increase in the relative amount of C17:cyclopropane fatty acid yield better survival after lyophilization
-
-
?
additional information
?
-
-
growth conditions that result in an increase in the relative amount of C17:cyclopropane fatty acid yield better survival after lyophilization
-
-
?
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
-
-
(1R,7aR)-hexahydro-1H-pyrrolizin-1-ylmethyl 4-hydroxy-3,5-dimethoxybenzoate
-
-
5,5'-dithiobis-(2-nitrobenzoic acid)
-
substrate does not protect against inactivation
additional information
-
identification of new inhibitors of Escherichia coli cyclopropane fatty acid synthase using a colorimetric assay
-
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine
-
this compound is also found to completely inhibit cyclopropanation of the phospholipids in growing Escherichia coli cells in vivo, at 0.15 mM
5,5'-dithiobis(2-nitrobenzoic acid)
-
reversed by addition of dithiothreitol
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
bicarbonate
-
required. CFA synthase isolated and assayed in potassium bicarbonate buffer displayes more than 3-fold higher activity than in HEPES buffer
glycinebetaine
Eubacterium HX
-
activity is extremely low in vitro in 100 mM buffers, 100fold stimulation by exogenous addition of 2-3 M glycinebetaine
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Granuloma
Cyclopropane Modification of Trehalose Dimycolate Drives Granuloma Angiogenesis and Mycobacterial Growth through Vegf Signaling.
Infections
Mycobacterium tuberculosis controls host innate immune activation through cyclopropane modification of a glycolipid effector molecule.
Infections
Trans-cyclopropanation of mycolic acids on trehalose dimycolate suppresses Mycobacterium tuberculosis -induced inflammation and virulence.
Leishmaniasis, Cutaneous
Functional analysis of Leishmania cyclopropane fatty acid synthetase.
Starvation
Augmentation of cyclopropane fatty acid synthesis under stringent control in Escherichia coli.
Tuberculosis
Escherichia coli cyclopropane fatty acid synthase: is a bound bicarbonate ion the active-site base?
Tuberculosis
Molecular dynamics investigation of the active site dynamics of mycobacterial cyclopropane synthase during various stages of the cyclopropanation process.
Tuberculosis
Redundant function of cmaA2 and mmaA2 in Mycobacterium tuberculosis cis cyclopropanation of oxygenated mycolates.
Tuberculosis
Synthesis and evaluation of analogues of S-adenosyl-L-methionine, as inhibitors of the E. coli cyclopropane fatty acid synthase.
Tuberculosis
The mmaA2 gene of Mycobacterium tuberculosis encodes the distal cyclopropane synthase of the alpha-mycolic acid.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
additional information
-
Km (phospholipids): 0.5 mg/ml. Since a mixture of phospholipids prepared from an Escherichia coli K12 strain is used, the Km constant is only an apparent constant given in mg/mL unit rather than molar unit
-
0.0705
S-adenosyl-L-methionine
-
purification buffer/assay buffer: KHCO3/HEPES
0.0716
S-adenosyl-L-methionine
-
purification buffer/assay buffer: HEPES/KHCO3
0.0887
S-adenosyl-L-methionine
-
purification buffer/assay buffer: HEPES/HEPES
0.092
S-adenosyl-L-methionine
with Tris/NaHCO3, at pH 8.0 and 37°C
0.0997
S-adenosyl-L-methionine
-
purification buffer/assay buffer: KHCO3/KHCO3
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.071
S-adenosyl-L-methionine
with Tris/NaHCO3, at pH 8.0 and 37°C
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.77
S-adenosyl-L-methionine
with Tris/NaHCO3, at pH 8.0 and 37°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0011 - 0.0014
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
0.0011
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
-
inhibits CAFS in a mixed-type fashion with respect to phospholipids
0.0014
(12aS,12bS)-7-fluoro-2,3,5,12,12a,12b-hexahydro-1H,4H-11-oxa-3a,9b-diazabenzo[a]naphtho[2,1,8-cde]azulene
-
inhibits the CFAS non-competitively with respect to AdoMet
0.006
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine
-
apparent inhibition constant when the phospholipids are the variable substrates. Noncompetitive inhibitor with respect to both substrates
0.016
5'-S-[2-(decylamino)ethyl]-5'-thioadenosine