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Information on EC 2.1.1.77 - protein-L-isoaspartate(D-aspartate) O-methyltransferase and Organism(s) Pyrococcus furiosus and UniProt Accession Q8TZR3

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EC Tree
IUBMB Comments
D-Aspartate (but not L-aspartate) residues in proteins can also act as acceptors. Previously also listed as EC 2.1.1.24.
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This record set is specific for:
Pyrococcus furiosus
UNIPROT: Q8TZR3
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Word Map
The taxonomic range for the selected organisms is: Pyrococcus furiosus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
pcm-1, pcmt1, protein l-isoaspartyl methyltransferase, l-isoaspartyl methyltransferase, protein-l-isoaspartate (d-aspartate) o-methyltransferase, pimt1, protein l-isoaspartate (d-aspartate) o-methyltransferase, protein-l-isoaspartyl methyltransferase, protein isoaspartyl methyltransferase, protein l-isoaspartyl o-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D-aspartyl/L-isoaspartyl methyltransferase
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-
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L-aspartyl/L-isoaspartyl protein methyltransferase
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-
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L-isoaspartyl/D-aspartyl protein carboxyl methyltransferase
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methyltransferase, protein (D-aspartate)
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protein (L-isoaspartate) O-methyltransferase
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protein D-aspartate methyltransferase
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-
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protein L-isoaspartate methyltransferase
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-
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protein L-isoaspartyl methyltransferase
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-
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protein-beta-aspartate O-methyltransferase
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-
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protein-L-isoaspartate O-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:protein-L-isoaspartate O-methyltransferase
D-Aspartate (but not L-aspartate) residues in proteins can also act as acceptors. Previously also listed as EC 2.1.1.24.
CAS REGISTRY NUMBER
COMMENTARY hide
105638-50-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
S-adenosyl-L-methionine + KASA (D-Asp) LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + KASA-L-isoAsp-LAKY
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPF-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAPL-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAV-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-succinyl-AAVA-p-nitroanilide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + ovalbumin
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein D-aspartate
S-adenosyl-L-homocysteine + protein D-aspartate beta-methyl ester
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
?
S-adenosyl-L-methionine + succinamide
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + VYP-L-isoAsp-HA
S-adenosyl-L-homocysteine + ?
show the reaction diagram
-
-
-
-
?
additional information
?
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O-succinyl-L-homoserine, succinyl choline chloride, succinyl acetone, succinic acid and succinamic acid are no substrates
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-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
protein repair methyltransferase
-
?
S-adenosyl-L-methionine + protein L-isoaspartate
S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
active methyl-donating cofactor
adenosine
-
tightly bound cofactor
S-adenosyl-L-homocysteine
-
tightly bound cofactor
S-adenosyl-L-methionine
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-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5'-deoxy-5'-methylthioadenosine
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-
adenosine
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-
S-adenosylhomocysteine
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potent inhibitor at pH 4 and 7.5
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.023
KASA (D-Asp) LAKY
-
-
0.00026 - 0.021
KASA (isoAsp) LAKY
0.375 - 11
N-succinyl-AAVAp-nitroanilide
0.00463 - 0.166
ovalbumin
-
0.00341
S-adenosyl-L-methionine
-
68°C, pH 7.5
0.00053 - 0.008
VYP-L-isoAsp-HA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
85 - 95
-
little or no loss of activity
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned and expressed in Escherichia coli TOP10
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Griffith, S.C.; Sawaya, M.R.; Boutz, D.R.; Thapar, N.; Katz, J.E.; Clarke, S.; Yeates, T.O.
Crystal Structure of a Protein Repair Methyltransferase from Pyrococcus furiosus with its L-Isoaspartyl Peptide Substrate
J. Mol. Biol.
313
1103-1116
2001
Pyrococcus furiosus (Q8TZR3), Pyrococcus furiosus
Manually annotated by BRENDA team
Thapar, N.; Griffith, S.C.; Yeates, T.O.; Clarke, S.
Protein repair methyltransferase from the hyperthermophilic archaeon Pyrococcus furiosus: unusual methyl-accepting affinity for D-aspartyl and N-succinyl-containing peptides
J. Biol. Chem.
277
1058-1065
2002
Arabidopsis thaliana, Caenorhabditis elegans, Escherichia coli, Homo sapiens, Mus musculus, Pyrococcus furiosus, Thermotoga maritima
Manually annotated by BRENDA team