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Information on EC 2.1.1.68 - caffeate O-methyltransferase and Organism(s) Oryza sativa and UniProt Accession Q6ZD89

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.68 caffeate O-methyltransferase
IUBMB Comments
3,4-Dihydroxybenzaldehyde and catechol can act as acceptors, but more slowly.
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This record set is specific for:
Oryza sativa
UNIPROT: Q6ZD89
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The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
caffeic acid o-methyltransferase, bmr12, atomt1, caffeate o-methyltransferase, oscomt1, caffeic acid o-methyltransferase 1, caffeate 3-o-methyltransferase, caffeic acid/5-hydroxyferulic acid 3/5-o-methyltransferase, fgcomt1, o-methytransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
caffeic acid O-methyltransferase
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caffeate 3-O-methyltransferase
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-
-
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caffeate methyltransferase
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-
-
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caffeic acid 3-O-methyltransferase
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caffeic acid O-methyltransferase
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COMT
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OsCOMT1
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previously reported as NOMT
S-adenosyl-L-methionine:caffeic acid-O-methyltransferase
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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-
-
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3,4-dihydroxy-trans-cinnamate 3-O-methyltransferase
3,4-Dihydroxybenzaldehyde and catechol can act as acceptors, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
50936-45-3
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
show the reaction diagram
-
-
-
?
eriodictyol
?
show the reaction diagram
-
best substrate, 131% relative activity
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-
?
luteolin
?
show the reaction diagram
-
88% relative activity
-
-
?
myricetin
?
show the reaction diagram
-
75% relative activity
-
-
?
quercetin
?
show the reaction diagram
-
71% relative activity
-
-
?
S-adenosyl-L-methionine + caffeic acid
?
show the reaction diagram
-
100% relative activity
-
-
?
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + isorhamnetin
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
show the reaction diagram
-
-
-
-
?
additional information
?
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no acitivity with p-coumaric acid flavanones, naringenin, homoeriodictyol, hesperetin flavonols, kaempferol, isorhametin flavones, apigenin and chrysoeriol
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + caffeic acid
S-adenosyl-L-homocysteine + ferulic acid
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + N-acetylserotonin
S-adenosyl-L-homocysteine + melatonin
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + quercetin
S-adenosyl-L-homocysteine + isorhamnetin
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + serotonin
S-adenosyl-L-homocysteine + 5-methoxytryptamine
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
caffeic acid
23% residual activity at 0.1 mM
quercetin
23% residual activity at 0.005 mM
Ba2+
-
relative activity 70% of control
Ca2+
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relative activity 58% of control
Co2+
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strongly inhibits, relative activity 7% of control
Cu2+
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relative activity 0% of control
EDTA
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relative activity 77% of control
Hg2+
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relative activity 0% of control
Mg2+
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relative activity 64% of control
Mn2+
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relative activity 24% of control
Zn2+
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relative activity 9% of control
additional information
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no increase in the expression of OsCOMT1 mRNA detected in CuCl2-, jasmonic acid and UV treated leaves
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.243
N-acetylserotonin
at pH 7.8 and 37°C
0.069
caffeic acid
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when S-adenosyl-L-methionine is supplied at a constant concentration of 1 mM
0.051
S-adenosyl-L-methionine
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when caffeic acid is supplied at a constant concentration of 1 mM
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
transgenic rice plants overexpressing the enzyme show an increase in melatonin levels
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
-
purified enzyme, SDS-PAGE
42300
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sequence analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-80°C, Tris-HCl buffer, 10% glycerol and 2-mercaptoethanol, pH 8.0
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography and Superdex S200 gel filtration
by His-Bind column chromatography
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Ni-NTA column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells and calli from Oryza sativa cultivar Dongjin
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expressed in Escherichia coli BL21(DE3)pLysS cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Lin, F.; Yamano, G.; Hasegawa, M.; Anzai, H.; Kawasaki, S.; Kodama, O.
Cloning and functional analysis of caffeic acid 3-O-methyltransferase from rice (Oryza sativa)
J. Pestic. Sci.
31
47-53
2006
Oryza sativa
-
Manually annotated by BRENDA team
Byeon, Y.; Choi, G.H.; Lee, H.Y.; Back, K.
Melatonin biosynthesis requires N-acetylserotonin methyltransferase activity of caffeic acid O-methyltransferase in rice
J. Exp. Bot.
66
6917-6925
2015
Oryza sativa (Q6ZD89), Oryza sativa
Manually annotated by BRENDA team
Choi, G.H.; Lee, H.Y.; Back, K.
Chloroplast overexpression of rice caffeic acid O-methyltransferase increases melatonin production in chloroplasts via the 5-methoxytryptamine pathway in transgenic rice plants
J. Pineal Res.
63
e12412
2017
Oryza sativa
Manually annotated by BRENDA team