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Information on EC 2.1.1.61 - tRNA 5-(aminomethyl)-2-thiouridylate-methyltransferase and Organism(s) Mus musculus and UniProt Accession Q9DAT5

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IUBMB Comments
This enzyme specifically adds the terminal methyl group of 5-[(methylamino)methyl]-2-thiouridylate.
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This record set is specific for:
Mus musculus
UNIPROT: Q9DAT5
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Word Map
The taxonomic range for the selected organisms is: Mus musculus
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
trna 5-methylaminomethyl-2-thiouridylate methyltransferase, 5-methylaminomethyl-2-thiouridylate methyltransferase, 5-methylaminomethyl-2-thiouridylate-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5-methylaminomethyl-2-thiouridylate-methyltransferase
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methyltransferase, transfer ribonucleate 5-methylaminomethyl-2-thiouridylate 5-
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MTO2
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MTU1
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S-adenosyl-L-methionine:tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
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transfer ribonucleate 5-methylaminomethyl-2-thiouridylate 5-methyltransferase
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TRMU
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tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
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tRNA 5-methylaminomethyl-2-thiouridylate 5'-methyltransferase
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tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA 5-(aminomethyl)-2-thiouridylate N-methyltransferase
This enzyme specifically adds the terminal methyl group of 5-[(methylamino)methyl]-2-thiouridylate.
CAS REGISTRY NUMBER
COMMENTARY hide
39391-17-8
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA
S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-aminomethyl-2-thiouridine in tRNA
S-adenosyl-L-homocysteine + 5-methylaminomethyl-2-thiouridylate in tRNA
show the reaction diagram
enzyme plays a role in tRNA modification and protein synthesis, pivotal in the fidelity of the translational process
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?
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
8.7
amino acid sequence calculation
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene trmU
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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cell death and apoptosis are significantly increased after neomycin injury in cells with downregulated enzyme expression
physiological function
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the enzyme regulates mitochondrial function and reactive oxygen species levels
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MTU1_MOUSE
417
0
47240
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47240
x * 47240, amino acid sequence calculation
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 47240, amino acid sequence calculation
?
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x * 46000, SDS-PAGE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene trmU, DNA and amino acid sequence determination and analysis, expression in NIH3T3 cells mitochondria as N-terminal GFP-fusion protein
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
enzyme expression is significantly decreased after 24 h neomycin treatment
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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the enzyme is a target for the prevention of aminoglycoside-induced hair cell death
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yan, Q.; Guan, M.X.
Identification and characterization of mouse TRMU gene encoding the mitochondrial 5-methylaminomethyl-2-thiouridylate-methyltransferase
Biochim. Biophys. Acta
1676
119-126
2004
Mus musculus (Q9DAT5), Mus musculus
Manually annotated by BRENDA team
He, Z.; Sun, S.; Waqas, M.; Zhang, X.; Qian, F.; Cheng, C.; Zhang, M.; Zhang, S.; Wang, Y.; Tang, M.; Li, H.; Chai, R.
Reduced TRMU expression increases the sensitivity of hair-cell-like HEI-OC-1 cells to neomycin damage in vitro
Sci. Rep.
6
29621
2016
Mus musculus
Manually annotated by BRENDA team