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IUBMB CommentsdCMP is methylated by formaldehyde in the presence of tetrahydrofolate. CMP, dCTP and CTP can act as acceptors, but more slowly.
The expected taxonomic range for this enzyme is: Xanthomonas oryzae
Synonyms
deoxycytidylate methyltransferase, more
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dCMP methyltransferase
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deoxycytidylate methyltransferase
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methyltransferase, deoxycytidylate
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5,10-methylenetetrahydrofolate + dCMP = dihydrofolate + deoxy-5-methylcytidylate
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methyl group transfer
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5,10-methylenetetrahydrofolate:dCMP C-methyltransferase
dCMP is methylated by formaldehyde in the presence of tetrahydrofolate. CMP, dCTP and CTP can act as acceptors, but more slowly.
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5,10-methylenetetrahydrofolate + CMP
dihydrofolate + 5-methylcytidylate
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Substrates: -
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5,10-methylenetetrahydrofolate + CTP
dihydrofolate + 5-methylcytidine 5'-triphosphate
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Substrates: low activity
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5,10-methylenetetrahydrofolate + dCMP
dihydrofolate + deoxy-5-methylcytidylate
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Substrates: -
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5,10-methylenetetrahydrofolate + dCTP
dihydrofolate + deoxy-5-methylcytidine 5'-triphosphate
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Substrates: low activity
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additional information
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additional information
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Substrates: dCMP is methylated by formaldehyde in the presence of tetrahydrofolate
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additional information
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Substrates: dCMP is methylated by formaldehyde in the presence of tetrahydrofolate
Products: -
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5,10-methylenetetrahydrofolate + dCMP
dihydrofolate + deoxy-5-methylcytidylate
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Substrates: -
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Ca2+
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25 mM, slight stimulation
Cu2+
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below 1 mM, stimulation
Mn2+
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has a similar effect in stimulation but not as strong as Mg2+
Zn2+
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below 1 mM, stimulation
additional information
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among the buffers tested, 6-morpholinoethane sulfonate/Mg2+ is the best
Mg2+
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required
Mg2+
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stimulates below 25 mM
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0.0714
5,10-methylenetetrahydrofolate
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additional information
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phage Xp12-infected
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brenda
Highest Expressing Human Cell Lines
Cell Line Links
Gene Links
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50
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3 min, 90% loss of activity
70
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3 min, complete loss of activity
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4°C, stable for at least 2 weeks
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phage Xp12-infected, partial
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Kuo, T.T.; Tu, J.
Enzymatic synthesis of deoxy-5-methyl-cytidylic acid replacing deoxycytidylic acid in Xanthomonas oryzae phage Xp12 DNA
Nature
263
615
1976
Xanthomonas oryzae
brenda
Wang, R.Y.H.; Huang, L.H.; Ehrlich, M.
Enzymatic conversion of deoxycytidine 5-monophosphate to 5-methyldeoxycytidine 5-triphosphate
Anal. Biochem.
122
89-93
1982
Xanthomonas oryzae
brenda
Feng, T.Y.; Tu, J.; Kuo, T.T.
Characterization of deoxycytidylate methyltransferase in Xanthomonas oryzae infected with bacteriophage Xp12
Eur. J. Biochem.
87
29-36
1978
Xanthomonas oryzae
brenda
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