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IUBMB Comments The enzyme, characterized from the bacterium Eubacterium limosum , is a component of a system that transfers a methyl group from L -proline betaine to tetrahydrofolate, as part of an L -proline betaine degradation pathway. The resulting 5-methyltetrahydrofolate is processed to acetyl-CoA via the Wood—Ljungdahl pathway.
Reaction Schemes
+
a [Co(I) quaternary-amine-specific corrinoid protein]
=
a [methyl-Co(III) quaternary-amine-specific corrinoid protein]
+
Synonyms mtpB , proline betaine methyltransferase, proline betaine:corrinoid methyltransferase,
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proline betaine methyltransferase
proline betaine:corrinoid methyltransferase
mtpB
-
-
-
-
proline betaine methyltransferase
-
proline betaine methyltransferase
-
-
proline betaine:corrinoid methyltransferase
-
proline betaine:corrinoid methyltransferase
-
-
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L-proline betaine + a [Co(I) quaternary-amine-specific corrinoid protein] = a [methyl-Co(III) quaternary-amine-specific corrinoid protein] + N-methyl-L-proline
-
-
-
-
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demethylation
anoxic proline betaine demethylation
demethylation
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anoxic proline betaine demethylation
-
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L-proline betaine:[Co(I) quaternary-amine-specific corrinoid protein] Co-methyltransferase
The enzyme, characterized from the bacterium Eubacterium limosum, is a component of a system that transfers a methyl group from L-proline betaine to tetrahydrofolate, as part of an L-proline betaine degradation pathway. The resulting 5-methyltetrahydrofolate is processed to acetyl-CoA via the Wood---Ljungdahl pathway.
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glycine betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-glycine
Substrates: low activity
?
L-carnithine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] ?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
phosphocholine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + ?
trans-4-hydroxy-L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + ?
Substrates: low activity
?
additional information
?
-
L-carnithine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] ?
Substrates: low activity
?
L-carnithine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] ?
Substrates: low activity
?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: -
?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: highest activity
?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: -
?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: highest activity
?
phosphocholine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + ?
Substrates: low activity
?
phosphocholine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + ?
Substrates: low activity
?
additional information
?
-
Substrates: no activity with cob(I)alamin or choline
-
additional information
?
-
Substrates: no activity with cob(I)alamin or choline
-
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L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: -
?
L-proline betaine + [Co(I) quaternary-amine-specific corrinoid protein MtqC]
[methyl-Co(III) quaternary-amine-specific corrinoid protein MtqC] + N-methyl-L-proline
Substrates: -
?
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8
L-proline betaine
at pH 7.2 and 37°C
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2.83
L-proline betaine
at pH 7.2 and 37°C
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
Filter by:
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metabolism
the enzyme, the corrinoid protein MtqC, and the methylcorrinoid:tetrahydrofolate methyltransferase MtqA catalyze tetrahydrofolate methylation with L-proline betaine, thereby forming a key intermediate in the Wood-Ljungdahl acetogenesis pathway
metabolism
-
the enzyme, the corrinoid protein MtqC, and the methylcorrinoid:tetrahydrofolate methyltransferase MtqA catalyze tetrahydrofolate methylation with L-proline betaine, thereby forming a key intermediate in the Wood-Ljungdahl acetogenesis pathway
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MTPB_EUBLI
485
0
54434
Swiss-Prot
other Location (Reliability: 2 )
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4°C, in 10% or 50% (v/v) glycerol, at least 2 weeks, activity is maximally retained
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Superose 12 column chromatography
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expressed in Escherichia coli BL21(DE3) cells
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Picking, J.; Behrman, E.; Zhang, L.; Krzycki, J.
MtpB, a member of the MttB superfamily from the human intestinal acetogen Eubacterium limosum, catalyzes proline betaine demethylation
J. Biol. Chem.
294
13697-13707
2019
Eubacterium limosum (P0DX09), Eubacterium limosum ATCC 8486 (P0DX09)
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