We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments Puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.
The enzyme appears in viruses and cellular organisms
Synonyms
O-demethylpuromycin methyltransferase,
more
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
O-demethylpuromycin methyltransferase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + O-demethylpuromycin = S-adenosyl-L-homocysteine + puromycin
puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxyl group with an amino group and acylating this with 4-O-methyltyrosine
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
methyl group transfer
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine:O-demethylpuromycin O-methyltransferase
Puromycin is the antibiotic derived from N6-dimethyladenosine by replacing the 3'-hydroxy group with an amino group and acylating this with 4-O-methyltyrosine.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + N-acetyl-O-demethylpuromycin
S-adenosyl-L-homocysteine + N-acetylpuromycin
-
-
-
-
?
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
additional information
?
-
-
L-tyrosine, L-tyrosine ethyl ester, L-tyrosine amide, catechol and epinephrine are not substrates
-
-
?
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
-
-
-
-
?
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
-
last step in the biosynthesis of puromycin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
-
-
-
-
?
S-adenosyl-L-methionine + O-demethylpuromycin
S-adenosyl-L-homocysteine + puromycin
-
last step in the biosynthesis of puromycin
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
D-glucose
-
reduced enzyme form, represses puromycin synthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.21
O-demethylpuromycin
-
-
0.01
S-adenosyl-L-methionine
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
8
-
broad pH optimum over the range 7.0-9.0 with a maximum at pH 8.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
mutant strain ATCC 12462
-
-
brenda
wild-type organism ATCC 12461
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
68000
-
sucrose density gradient centrifugation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.5
-
very unstable at pH 7.5 and 38°C
485314
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
37
-
rapidly inactivated at 37°C, very unstable even at low temperatures, enzyme activity is destroyed upon boiling
38
-
very unstable at 38°C and pH 7.5
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
stabilized by addition of S-adenosyl-L-methionine
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-65°C, can be stored for several months without loss of activity
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase, cloning of the complete gene cluster for puromycin and expression in heterologous hosts
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
medicine
-
product puromycin is an antibiotic and antitumor agent
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Rao, M.M.; Rebello, P.F.; Pogell, B.M.
Biosynthesis of puromycin in Streptomyces alboniger. Enzymatic methylation of O-demethylpuromycin
J. Biol. Chem.
244
112-118
1969
Streptomyces alboniger
brenda
Pogell, B.M.
S-Adenosylmethionine: O-demethylpuromycin O-methyltransferase
Methods Enzymol.
43
508-515
1975
Streptomyces alboniger
brenda
Sankaran, L.; Pogell, B.M.
Biosynthesis of puromycin in Streptomyces alboniger: regulation and properties of O-demethylpuromycin O-methyltransferase
Antimicrob. Agents Chemother.
8
721-732
1975
Streptomyces alboniger
brenda
Vara, J.; Perez-Gonzalez, J.A.; Jimenez, A.
Biosynthesis of puromycin by Streptomyces alboniger: Characterization of puromycin N-acetyltransferase
Biochemistry
24
8074-8081
1985
Streptomyces alboniger
brenda
Lacalle, R.A.; Ruiz, D.; Jimenez, A
Molecular analysis of the dmpM gene encoding an O-demethyl puromycin O-methyltransferase from Streptomyces alboniger
Gene
109
55-61
1991
Streptomyces alboniger
brenda
Select items on the left to see more content.
html completed