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Information on EC 2.1.1.37 - DNA (cytosine-5-)-methyltransferase and Organism(s) Rattus norvegicus and UniProt Accession Q9Z330

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.37 DNA (cytosine-5-)-methyltransferase
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Select one or more organisms in this record: ?
This record set is specific for:
Rattus norvegicus
UNIPROT: Q9Z330 not found.
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Word Map
The taxonomic range for the selected organisms is: Rattus norvegicus
The enzyme appears in selected viruses and cellular organisms
Synonyms
dnmt1, dnmt3a, dna methyltransferase, dnmt3b, dna methyltransferase 1, dnmt2, dna mtase, nsun2, dna methylase, dna methyltransferase 3a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C5-MTase
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-
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cytosine 5-methyltransferase
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cytosine DNA methylase
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cytosine DNA methyltransferase
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cytosine-specific DNA methyltransferase
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-
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DCMT
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-
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deoxyribonucleate methylase
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-
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deoxyribonucleate methyltransferase
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deoxyribonucleic (cytosine-5-)-methyltransferase
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deoxyribonucleic acid (cytosine-5-)-methyltransferase
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-
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deoxyribonucleic acid methylase
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deoxyribonucleic acid methyltransferase
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deoxyribonucleic acid modification methylase
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deoxyribonucleic methylase
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DNA 5-cytosine methylase
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-
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DNA cytosine c5 methylase
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-
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DNA cytosine methylase
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DNA methylase
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-
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DNA methyltransferase
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DNA transmethylase
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DNA-cytosine 5-methylase
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-
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DNA-cytosine methyltransferase
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EcoRI methylase
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methylphosphotriester-DNA methyltransferase
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methyltransferase, deoxyribonucleate
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type II DNA methylase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
methyl group transfer
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:DNA (cytosine-5-)-methyltransferase
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CAS REGISTRY NUMBER
COMMENTARY hide
9037-42-7
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
-
-
?
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
S-adenosyl-L-methionine + DNA
S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
DNA + S-adenosyl-L-methionine
DNA containing 5-methylcytosine + S-adenosyl-L-homocysteine
show the reaction diagram
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DNMT1 is crucial for cell survival
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-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
putrescine
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1 mM, 42% inhibition
S-adenosyl-L-homocysteine
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potent competitive inhibitor
spermidine
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1 mM, 59% inhibition
spermine
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1 mM, 97% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00025
S-adenosyl-L-methionine
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in presence of Micrococcus luteus DNA
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00033
S-adenosyl-L-homocysteine
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the enzyme is highly expressed in placenta during mid-to late-pregnancy
Manually annotated by BRENDA team
the enzyme is regulated at the posttranscriptional level in terminally differentiating Rcho-1 cell
Manually annotated by BRENDA team
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different levels of global methylation in the adult rat dentate gyrus and CA1 region in comparison with the CA2 and CA3 regions. mRNA levels of DNA methyltransferases exhibit similar regional specificity and are correlated with global DNA methylation levels
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
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DNMT1 is crucial for cell survival, as in its absence cells undergo arrest in G1/S, a DNA damage response is triggered, and the cells undergo mitotic catastrophe
physiological function
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DNA methylation may play a dynamic physiological role in the adult brain, possibly, the DNA methylation pattern is undergoing constant remodeling and requires the presence of DNA methyltransferases to allow adaptation at the level of the DNA methylation pattern in response to environmental cues
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DNMT1_RAT
1622
0
182774
Swiss-Prot
Mitochondrion (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
115000
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glycerol gradient centrifugation
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, the purified enzyme preparation is stable for at least 6 months
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4°C, the purified enzyme preparation is rather unstable
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sneider, T.W.; Teague, W.M.; Rogachevsky, L.M.
S-Adenosylmethionine: DNA-cytosine 5-methyltransferase from a Novikoff rat hepatoma cell line
Nucleic Acids Res.
2
1685-1700
1975
Rattus norvegicus
Manually annotated by BRENDA team
Simon, D.; Grunert, F.; v.Acken, U.; Dring, H.P.; Krger, H.
DNA-methylase from regenerating rat liver: purification and characterisation
Nucleic Acids Res.
5
2153-2167
1978
Rattus norvegicus
Manually annotated by BRENDA team
Cox, R.
Polyamines inhibit DNA methylation in vitro
Biochem. Biophys. Res. Commun.
86
594-598
1979
Rattus norvegicus
Manually annotated by BRENDA team
Deng, J.; Szyf, M.
Downregulation of DNA (cytosine-5-)methyltransferase is a late event in NGF-induced PC12 cell differentiation
Mol. Brain Res.
71
23-31
1999
Rattus norvegicus
Manually annotated by BRENDA team
Kimura, H.; Takeda, T.; Tanaka, S.; Ogawa, T.; Shiota, K.
Expression of rat DNA (cytosine-5) methyltransferase (DNA MTase) in rodent trophoblast giant cells: molecular cloning and characterization of rat DNA MTase
Biochem. Biophys. Res. Commun.
253
495-501
1998
Rattus norvegicus (Q9Z330)
Manually annotated by BRENDA team
Brown, S.E.; Weaver, I.C.; Meaney, M.J.; Szyf, M.
Regional-specific global cytosine methylation and DNA methyltransferase expression in the adult rat hippocampus
Neurosci. Lett.
440
49-53
2008
Rattus norvegicus
Manually annotated by BRENDA team