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Information on EC 2.1.1.352 - 3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase and Organism(s) Papaver somniferum and UniProt Accession I3PLQ6

for references in articles please use BRENDA:EC2.1.1.352
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IUBMB Comments
This activity is part of the noscapine biosynthesis pathway, as characterized in the plant Papaver somniferum (opium poppy). It is catalysed by heterodimeric complexes of the OMT2 gene product and the product of either OMT3 or 6OMT. OMT2 is the catalytic subunit in both complexes.
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This record set is specific for:
Papaver somniferum
UNIPROT: I3PLQ6
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The taxonomic range for the selected organisms is: Papaver somniferum
The expected taxonomic range for this enzyme is: Papaver somniferum
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:3-O-acetyl-4'-O-demethylpapaveroxine 4'-O-methyltransferase
This activity is part of the noscapine biosynthesis pathway, as characterized in the plant Papaver somniferum (opium poppy). It is catalysed by heterodimeric complexes of the OMT2 gene product and the product of either OMT3 or 6OMT. OMT2 is the catalytic subunit in both complexes.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-O-acetyl-4'-O-demethylpapaveroxine
S-adenosyl-L-homocysteine + 3-O-acetylpapaveroxine
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 3-O-acetyl-4'-O-demethylpapaveroxine
S-adenosyl-L-homocysteine + 3-O-acetylpapaveroxine
show the reaction diagram
this activity is part of the noscapine biosynthesis pathway
-
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
I3PLQ6: 4'-O-desmethyl-3-O-acetylpapaveroxine O-methyltransferase subunit MT2, I3PLQ7: 4'-O-desmethyl-3-O-acetylpapaveroxine O-methyltransferase subunit MT3
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
this activity is part of the noscapine biosynthesis pathway
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
SOMT2_PAPSO
356
0
39087
Swiss-Prot
other Location (Reliability: 3)
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
heterodimer
complex of the OMT2 gene product and the product of either OMT3 or 6OMT. OMT2 is the catalytic subunit in both complexes. 6OMT shares 81% amino acid sequence identity with OMT3
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
S122Y
profound negative effects on dimerization and activity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, Y.; Smolke, C.
Engineering biosynthesis of the anticancer alkaloid noscapine in yeast
Nat. Commun.
7
12137
2016
Papaver somniferum (I3PLQ6)
Manually annotated by BRENDA team
Park, M.; Chen, X.; Lang, D.; Ng, K.; Facchini, P.
Heterodimeric O-methyltransferases involved in the biosynthesis of noscapine in opium poppy
Plant J.
95
252-267
2018
Papaver somniferum (I3PLQ6)
Manually annotated by BRENDA team