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Information on EC 2.1.1.33 - tRNA (guanine46-N7)-methyltransferase and Organism(s) Saccharomyces cerevisiae and UniProt Accession Q12009
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2.1.1.33 tRNA (guanine46-N7)-methyltransferaseIUBMB Comments
The enzyme specifically methylates guanine46 at N7 in tRNA.
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Word Map
- 2.1.1.33
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methyltransferase-like
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n7-methylguanosine
- cyp27b1
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mettl21b
- trnaphe
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mescs
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kidney-specific
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methylome
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submodules
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methyl-transfer
- pharmacology
The taxonomic range for the selected organisms is: Saccharomyces cerevisiae
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
+
guanine46 in tRNA
=
+
N7-methylguanine46 in tRNA
Synonyms
mettl1, m7g-methyltransferase, methyltransferase like 1, trna (m7g46) methyltransferase, m7g46 methyltransferase trm8p/trm82p, transfer rna (m7g46) methyltransferase, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
7-methylguanine transfer ribonucleate methylase
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m7G-methyltransferase
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methyltransferase, transfer ribonucleate guanine 7-
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N7-methylguanine methylase
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transfer ribonucleate guanine 7-methyltransferase
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tRNA guanine 7-methyltransferase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
methyl group transfer
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:tRNA (guanine-N7-)-methyltransferase
The enzyme specifically methylates guanine46 at N7 in tRNA.
CAS REGISTRY NUMBER
COMMENTARY
37257-00-4
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
additional information
additional information
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complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, but Trm82 is not an absolute requirement for activity in vitro, Trm8 may be the catalytically active subunit
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-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
Q03774; Q12009
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-
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptor: methyl-deficient tRNA from E. coli
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptors: tRNACys, tRNATrp, tRNAPro, tRNAMet, tRNAMet-i and 2 of each tRNAPhe, tRNAVal and tRNALys, methylation at G46 in the extra loop
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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methyl group acceptor: yeast pre-tRNAPhe
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo
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?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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-
-
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?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
Q03774; Q12009
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-
-
?
S-adenosyl-L-methionine + guanine46 in tRNA
S-adenosyl-L-homocysteine + N7-methylguanine46 in tRNA
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complex of 2 proteins Trm8 and Trm82 is required for efficient m7G-methyltransferase activity in vitro and in vivo, tRNA site G46 is methylated in vivo
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00035
guanine46 in tRNA
Q03774; Q12009
pH 7.5, 30°C, tRNA variant with disruption of tertiary base pairs between D- and T-arms
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0.0005
guanine46 in tRNA
Q03774; Q12009
pH 7.5, 30°C, tRNA variant with disruption of C13-G22-G46 tertiary base pair
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0.0014
guanine46 in tRNA
Q03774; Q12009
pH 7.5, 30°C, tRNA variant with deletion of the anticodon-arm
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0.0037
guanine46 in tRNA
Q03774; Q12009
pH 7.5, 30°C, tRNA variant with deletion of the aminoacyl-stem
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
Q03774; Q12009
m7G46 methyltransferase Trm8p/Trm82p acts as a hub of synthetic interactions with several tRNA modification enzymes, resulting in temperature-sensitive growth
physiological function
Q03774; Q12009
Trm8p is required for m7G modification. Trm82p is required to maintain cellular levels of Trm8p and to stabilize Trm8p in an active conformation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). A0A6A5PXN6_YEASX
286
0
33391
TrEMBL
other Location (Reliability: 2)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
33000
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1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE
50000
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1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
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1 * 33000 (subunit Trm8) + 1 * 50000 (subunit Trm82), association of the Trm8 and Trm82 subunits is translationally controlled in living cells, SDS-PAGE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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strains lacking either Trm8 or Trm82 yield extracts with no detectable enzyme activity, and have severely reduced m7G-modified tRNA in vivo
additional information
Q03774; Q12009
strains lacking Trm8p/Trm82p tRNA m7G methyltransferase, are temperature-sensitive in synthetic media containing glycerol, trm82-delta strain shows reduced levels of Trm8p, but not of its mRNA, the growth phenotype of trm8 mutants correlates with lack of tRNA m7G methyltransferase activity in vitro and in vivo, bacterial Trm8p orthologs complement the trm8-delta trm82-delta growth phenotype and restore m7G-methyltransferase activity in vitro and in vivo
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
freezing without antifreeze agents inactivates, 30% ethylene glycol at -20°C stabilizes
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography, Sephadex G-25 gel filtration, ammonium sulfate precipitation, and DE52 column chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a wheat germ cell-free translation system, active Trm8-Trm82 heterodimer is only synthesized under conditions, in which both Trm8 and Trm82 mRNAs are cotranslated
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ORF YDL201w encodes Trm8 and ORF YDR165w encodes Trm82, both proteins form a complex with m7G-methyltransferase activity, Trm8 contains a binding domain for S-adenosyl-L-methionine: GXGXG, coexpression of Trm8/Trm82 in Escherichia coli BL21 Codon Plus (DE3) as His6-fusion proteins under control of the Ptac-promoter
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Bjrk, G.R.; Svensson, I.
Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae
Eur. J. Biochem.
9
207-215
1969
Saccharomyces cerevisiae, Saccharomyces cerevisiae D84
Alexandrov, A.; Martzen, M.R.; Phizicky, E.M.
Two proteins that form a complex are required for 7-methylguanosine modification of yeast tRNA
RNA
8
1253-1266
2002
Homo sapiens, Saccharomyces cerevisiae
Matsumoto, K.; Toyooka, T.; Tomikawa, C.; Ochi, A.; Takano, Y.; Takayanagi, N.; Endo, Y.; Hori, H.
RNA recognition mechanism of eukaryote tRNA (m(7)G46) methyltransferase (Trm8-Trm82 complex)
FEBS Lett.
581
1599-1604
2007
Saccharomyces cerevisiae (Q03774 and Q12009), Saccharomyces cerevisiae
Alexandrov, A.; Chernyakov, I.; Gu, W.; Hiley, S.L.; Hughes, T.R.; Grayhack, E.J.; Phizicky, E.M.
Rapid tRNA decay can result from lack of nonessential modifications
Mol. Cell
21
87-96
2006
Saccharomyces cerevisiae (Q03774 and Q12009)
Alexandrov, A.; Grayhack, E.J.; Phizicky, E.M.
tRNA m7G methyltransferase Trm8p/Trm82p: evidence linking activity to a growth phenotype and implicating Trm82p in maintaining levels of active Trm8p
RNA
11
821-830
2005
Saccharomyces cerevisiae (Q03774 and Q12009)
EXTERNAL LINKS (specific for EC-Number 2.1.1.33)
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