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Information on EC 2.1.1.300 - pavine N-methyltransferase and Organism(s) Thalictrum flavum and UniProt Accession C3SBW0

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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.300 pavine N-methyltransferase
IUBMB Comments
The enzyme, isolated from the plant Thalictrum flavum, also methylates (R,S)-stylopine and (S)-scoulerine (11%) with lower activity (14% and 11%, respectively).
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Thalictrum flavum
UNIPROT: C3SBW0
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Word Map
The taxonomic range for the selected organisms is: Thalictrum flavum
The enzyme appears in selected viruses and cellular organisms
Synonyms
pavine n-methyltransferase, pavine nmt, pavnmt, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(+/-)-pavine N-methyltransferase
The enzyme, isolated from the plant Thalictrum flavum, also methylates (R,S)-stylopine and (S)-scoulerine (11%) with lower activity (14% and 11%, respectively).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (+/-)-pavine
S-adenosyl-L-homocysteine + N-methylpavine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + (R,S)-stylopine
S-adenosyl-L-homocysteine + N-methylstylopine
show the reaction diagram
-
-
-
-
?
S-adenosyl-L-methionine + (S)-scoulerine
S-adenosyl-L-homocysteine + N-methyl-(S)-scoulerine
show the reaction diagram
-
-
-
-
?
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
sitting-drop vapour-diffusion method, hanging-drop vapour-diffusion method at 16°C, crystallized in space group P2(1). The structure is solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with anomalous scattering method
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
heterologously expressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Jain, A.; Ziegler, J.; Liscombe, D.K.; Facchini, P.J.; Tucker, P.A.; Panjikar, S.
Purification, crystallization and X-ray diffraction analysis of pavine N-methyltransferase from Thalictrum flavum
Acta Crystallogr. Sect. F
64
1066-1069
2008
Thalictrum flavum
Manually annotated by BRENDA team