The enzyme from anise (Pimpinella anisum) is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8, and, in contrast to EC 2.1.1.146, (iso)eugenol O-methyltransferase, does not have activity with eugenol or chavicol.
The enzyme appears in viruses and cellular organisms
The enzyme from anise (Pimpinella anisum) is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8, and, in contrast to EC 2.1.1.146, (iso)eugenol O-methyltransferase, does not have activity with eugenol or chavicol.
Substrates: shows a 10fold preference for isoeugenol over eugenol The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Products: -
Substrates: the enzyme shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Products: -
Substrates: the enzyme prefers isoeugenol to t-anol by a factor of 2. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Products: -
Substrates: trans-anol i.e. 4-[(1E)-prop-1-en-1-yl]phenol. The enzyme prefers isoeugenol to t-anol by a factor of 2. It shows a 10fold preference for t-anol over chavicol. The enzyme is highly specific for substrates in which the double bond in the propenyl side chain is located between C7 and C8 Products: trans-anethole i.e. 1-methoxy-4-[(1E)-prop-1-en-1-yl]benzene
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
in developing fruits, enzyme activity levels increase until the wasty stage is reached and then dramatically decrease. Young leaves display higher levels of enzyme activity than old leaves
Koeduka, T.; Baiga, T.J.; Noel, J.P.; Pichersky, E.
Biosynthesis of t-anethole in anise: characterization of t-anol/isoeugenol synthase and an O-methyltransferase specific for a C7-C8 propenyl side chain
Gross, M.; Friedman, J.; Dudai, N.; Larkov, O.; Cohen, Y.; Bar, E.; Ravid, U.; Putievsky, E.; Lewinsohn, E.
Biosynthesis of estragole and t-anethole in bitter fennel (Foeniculum vulgare Mill. var. vulgare) chemotypes. Changes in SAM:phenylpropene O-methyltransferase activities during development