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Information on EC 2.1.1.278 - indole-3-acetate O-methyltransferase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FLN8

for references in articles please use BRENDA:EC2.1.1.278
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EC Tree
     2 Transferases
         2.1 Transferring one-carbon groups
             2.1.1 Methyltransferases
                2.1.1.278 indole-3-acetate O-methyltransferase
IUBMB Comments
Binds Mg2+. The enzyme is found in plants and is important for regulation of the plant hormone (indol-3-yl)acetate. The product, methyl (indol-3-yl)acetate is inactive as hormone .
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Select one or more organisms in this record:
This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FLN8
Word Map
  • 2.1.1.278
  • auxin
  • dissimilatory
  • hypocotyls
  • sulfite
  • sulfate-reducing
  • affiliated
  • phytohormone
  • rosette
  • dr5-gus
  • desulfovibrio
  • seine
  • estuary
  • mimicry
  • poplar
  • biogeochemical
  • semi-dwarfism
  • curling
  • curvature
  • hyponastic
  • methyltransferase1
  • mudflats
  • monophyletic
  • auxin-responsive
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
At5g55250, AtIAMT1, IAA carboxyl methyltransferase, IAA carboxylmethyltransferase, IAA methyltransferase, IAA-methyltransferase-1, IAMT, IAMT1, indole-3-acetic acid carboxyl methyltransferase, indole-3-acetic acid methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
At5g55250
298450
gene name
AtIAMT1
298450
-
IAA carboxyl methyltransferase
298450
-
IAA carboxylmethyltransferase
298450
-
IAA methyltransferase
298450
-
IAA-methyltransferase-1
298450
-
IAMT1
indole-3-acetic acid carboxyl methyltransferase
298450
-
indole-3-acetic acid methyltransferase
298450
-
indole-3-acetic acid-methyltransferase-1
298450
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:(indol-3-yl)acetate O-methyltransferase
Binds Mg2+. The enzyme is found in plants and is important for regulation of the plant hormone (indol-3-yl)acetate. The product, methyl (indol-3-yl)acetate is inactive as hormone [2].
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (indol-3-yl)acetate
S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + (indol-3-yl)acetate
S-adenosyl-L-homocysteine + methyl (indol-3-yl)acetate
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
binds Mg2+
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.013
(indol-3-yl)acetate
-
pH 7.5, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.028
(indol-3-yl)acetate
-
pH 7.5, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.15
(indol-3-yl)acetate
-
pH 7.5, 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
Sequence
IAMT1_ARATH
386
0
42054
Swiss-Prot
CRYSTALLIZATION/commentary
ORGANISM
UNIPROT
LITERATURE
the crystal structure of the enzyme is determined and refined to 2.75 A resolution
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
targeted nuclear expression of Triticum aestivum gene TaWRKY71-1, encoding a WRKY transcription factor, in Arabidopsis thaliana induces the expression if gene IAMT1. TaWRKY71-1 localizes in the nucleus and has no transcriptional activation activity. TaWRKY71-1 overexpression in Arabidopsis results in hyponastic rosette leaves, and the hyponastic strength is closely correlative with the transcription level of the transgene. The spongy mesophyll cells at abaxial side of leaves are drastically compacted by TaWRKY71-1 overexpression
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
CLONED/commentary
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
targeted nuclear expression of Triticum aestivum gene TaWRKY71-1, encoding a WRKY transcription factor, in Arabidopsis thaliana induces the expression if gene IAMT1
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Li, L.; Hou, X.; Tsuge, T.; Ding, M.; Aoyama, T.; Oka, A.; Gu, H.; Zhao, Y.; Qu, L.J.
The possible action mechanisms of indole-3-acetic acid methyl ester in Arabidopsis
Plant Cell Rep.
27
575-584
2008
Arabidopsis thaliana (Q9FLN8)
Manually annotated by BRENDA team
Zubieta, C.; Ross, J.R.; Koscheski, P.; Yang, Y.; Pichersky, E.; Noel, J.P.
Structural basis for substrate recognition in the salicylic acid carboxyl methyltransferase family
Plant Cell
15
1704-1716
2003
Arabidopsis thaliana (Q9FLN8)
Manually annotated by BRENDA team
Zhao, N.; Ferrer, J.L.; Ross, J.; Guan, J.; Yang, Y.; Pichersky, E.; Noel, J.P.; Chen, F.
Structural, biochemical, and phylogenetic analyses suggest that indole-3-acetic acid methyltransferase is an evolutionarily ancient member of the SABATH family
Plant Physiol.
146
455-467
2008
Arabidopsis thaliana (Q9FLN8), Oryza sativa Japonica Group (Q0J998)
Manually annotated by BRENDA team
Qin, G.; Gu, H.; Zhao, Y.; Ma, Z.; Shi, G.; Yang, Y.; Pichersky, E.; Chen, H.; Liu, M.; Chen, Z.; Qu, L.J.
An indole-3-acetic acid carboxyl methyltransferase regulates Arabidopsis leaf development
Plant Cell
17
2693-2704
2005
Arabidopsis thaliana, Arabidopsis thaliana (Q9FLN8)
Manually annotated by BRENDA team
Qin, Z.; Lv, H.; Zhu, X.; Meng, C.; Quan, T.; Wang, M.; Xia, G.
Ectopic expression of a wheat WRKY transcription factor gene TaWRKY71-1 results in hyponastic leaves in Arabidopsis thaliana
PLoS ONE
8
e63033
2013
Arabidopsis thaliana, Arabidopsis thaliana (Q9FLN8), Arabidopsis thaliana Col-0 (Q9FLN8)
Manually annotated by BRENDA team
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