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Information on EC 2.1.1.260 - rRNA small subunit pseudouridine methyltransferase Nep1 and Organism(s) Archaeoglobus fulgidus and UniProt Accession O29524

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EC Tree
IUBMB Comments
This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. It recognizes Psi914 in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi1191 in yeast 18S rRNA, and Psi1248 in human 18S rRNA.
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Archaeoglobus fulgidus
UNIPROT: O29524
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The taxonomic range for the selected organisms is: Archaeoglobus fulgidus
The expected taxonomic range for this enzyme is: Archaea, Eukaryota, Bacteria
Reaction Schemes
+
pseudouridine1191 in yeast 18S rRNA
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N1-methylpseudouridine1191 in yeast 18S rRNA
Synonyms
nep1p, essential for mitotic growth 1, n1-specific pseudouridine methyltransferase, nucleolar essential protein 1, nep1 methyltransferase, pseudouridine n1-methyltransferase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N1-specific pseudouridine methyltransferase
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pseudouridine N1-methyltransferase
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Nep1
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nucleolar essential protein 1
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:18S rRNA (pseudouridine1191-N1)-methyltransferase
This enzyme, which occurs in both prokaryotes and eukaryotes, recognizes specific pseudouridine residues (Psi) in small subunits of ribosomal RNA based on the local RNA structure. It recognizes Psi914 in 16S rRNA from the archaeon Methanocaldococcus jannaschii, Psi1191 in yeast 18S rRNA, and Psi1248 in human 18S rRNA.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + pseudouridine1191 in yeast 18S rRNA
S-adenosyl-L-homocysteine + N1-methylpseudouridine1191 in yeast 18S rRNA
show the reaction diagram
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?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
Nep1 is a SPOUT RNA methyltransferase, and can catalyze methylation at the N1 of pseudouridine, it is required for 18S rRNA maturation. Nep1 is also involved in assembly of Rps19, an SSU ribosomal protein. Functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis, structure-function relationship, overview
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant detagged wild-type and selenomethionine-labeled afNep1 dimer bound to S-adenosyl homocysteine, X-ray diffraction structure determination and analysis at 1.45-2.0 A resolution
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant wild-type and selenomethionine-labeled His6-tagged Nep1 from Escherichia coli strain RosettaTM-DE3-pLysS by nickel affinity chromatography, cleavage of the tag by TEV protease, and two steps of gel filtration to over 99% purity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression of wild-type and selenomethionine-labeled His6-tagged Nep1 in Escherichia coli strain RosettaTM-DE3-pLysS
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thomas, S.R.; Keller, C.A.; Szyk, A.; Cannon, J.R.; Laronde-Leblanc, N.A.
Structural insight into the functional mechanism of Nep1/Emg1 N1-specific pseudouridine methyltransferase in ribosome biogenesis
Nucleic Acids Res.
39
2445-2457
2011
Archaeoglobus fulgidus (O29524), Saccharomyces cerevisiae
Manually annotated by BRENDA team