Information on EC 2.1.1.245 - 5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase

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The expected taxonomic range for this enzyme is: Archaea, Bacteria

EC NUMBER
COMMENTARY hide
2.1.1.245
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RECOMMENDED NAME
GeneOntology No.
5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein Co-methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin = a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
methanogenesis from acetate
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SYSTEMATIC NAME
IUBMB Comments
5-methyltetrahydrosarcinapterin:corrinoid/iron-sulfur protein methyltransferase
Catalyses the transfer of a methyl group from the cobamide cofactor of a corrinoid/Fe-S protein to the N5 group of tetrahydrosarcinapterin. Forms, together with EC 1.2.7.4, carbon-monoxide dehydrogenase (ferredoxin) and EC 2.3.1.169, CO-methylating acetyl-CoA synthase, the acetyl-CoA decarbonylase/synthase complex that catalyses the demethylation of acetyl-CoA in a reaction that also forms CO2. This reaction is a key step in methanogenesis from acetate.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
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the enzyme reaction initiates the unusual biological organometallic reaction sequence that constitutes the Wood-Ljungdahl or reductive acetyl-CoA pathway
additional information
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin
show the reaction diagram
CH3-H4SPt + cob(I)amide-protein + H+
H4SPt + CH3-cob(III)amide-protein
show the reaction diagram
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r
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a [methyl-Co(III) corrinoid Fe-S protein] + tetrahydrosarcinapterin
a [Co(I) corrinoid Fe-S protein] + 5-methyltetrahydrosarcinapterin
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Co2+
CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE
Fe2+
CdhE contains approximately 4.5 mol of Fe, 2.7 mol of acid-labile sulfur, and 0.3 mol of hydroxocobalamin per mol of purified CdhE; in the iron-sulfur protein
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
methylcob(III)-amide
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product inhibition
tetrahydrofolate
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product inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
a [methyl-Co(III) corrinoid Fe-S protein]
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pH 7.6, 25°C
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0.002
tetrahydrosarcinapterin
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pH 7.6, 25°C
additional information
additional information
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single-turnover stopped-flow studies, steady-state and pre-steady-state kinetic studies at different pH values and by kinetic simulations, random Bi-Bi reaction mechanism, detailed overview
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
14.7
a [methyl-Co(III) corrinoid Fe-S protein]
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pH 7.6, 25°C
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14.7
tetrahydrosarcinapterin
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pH 7.6, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant CdhD from Escherichia coli strain BL21(DE3); recombinant CdhE from Escherichia coli strain BL21(DE3)
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
genes cdhE and cdhD, the cdhD gene is located upstream of cdhE, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3); genes cdhE and cdhD, the cdhD gene is located upstream of cdhE, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3)
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