Information on EC 2.1.1.243 - 2-ketoarginine methyltransferase

for references in articles please use BRENDA:EC2.1.1.243
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The expected taxonomic range for this enzyme is: Pseudomonas syringae

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EC NUMBER
COMMENTARY hide
2.1.1.243
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RECOMMENDED NAME
GeneOntology No.
2-ketoarginine methyltransferase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate = S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
3-methylarginine biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
S-adenosyl-L-methionine:5-carbamimidamido-2-oxopentanoate S-methyltransferase
The enzyme is involved in production of the rare amino acid 3-methylarginine, which is used by the epiphytic bacterium Pseudomonas syringae pv. syringae as an antibiotic against the related pathogenic species Pseudomonas syringae pv. glycinea.
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
pv. syringae 22d/93
UniProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine
physiological function
the enzyme is involved inthe biosynthesis of the rare amino acid 3-methylarginine, which is highly active against the closely related soybean pathogen Pseudomonas syringae pv. glycinea. Since these pathogens compete for the same habitat, Pss22d is a promising candidate for biocontrol of P. syringae pv. glycinea
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
show the reaction diagram
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NMR and mass spectrometric product analysis
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?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + 5-guanidino-2-oxopentanoate
S-adenosyl-L-homocysteine + 5-guanidino-3-methyl-2-oxopentanoate
show the reaction diagram
D5FKJ3
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?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
S-adenosyl-L-methionine
dependent on
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged MrsA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene mrsA, DNA and amino acid sequence determination and analysis, sequences of genes mrsA and mrsB reveal an overlapping region in the start/stop sequences, sequence comparisons, mrsA-ATGA-mrsB, overexpression of the His-tagged MrsA in Escherichia coli strain BL21(DE3)
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
Tn5 transposon mutagenesis
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LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.243)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)